Inhibition of fibrin polymerization by synthetic peptides corresponding to Аα195-205 and γ69-77 sites sites of fibrin molecule

Abstract

InhIbItIon of fIbrIn polymerIzatIon

Probing Fibrinogen Structure: Insights From Limited Proteolysis, Peptides, and Monoclonal Antibodies

“…Thus, using small peptides, was discovered the important role of the hinge sites of the coiled-coil region of the fibrin(ogen) molecule in the process of fibrin polymerization. It has been demonstrated that synthetic peptide А195-205 inhibits the stage of fibrin protofibril formation, while peptide 69-77 inhibits the lateral association of protofibrils [7]. The use of small molecules helps minimize steric constraints that may arise from their binding to the fibrin(ogen) molecule.…”

“…Scheme of fibrinogen molecule with some effectors (antibody, protease, peptide) targeted to the distinct parts of the molecule. Modified after: [13] I-3C antibody targeted to 122-134 residue of B-chain of fibrinogen[14]; BK134-G135 is a peptide bond that is cleaved during the formation of TSK-fragment of fibrinogen[15]; B125-135 is a synthetic peptide [16]; I-5A antibody targeted to 509-610 residue of A-chain of fibrinogen[17]; AA504-S505 -is a peptide bond that is cleaved by protease II from the culture medium of Bacillus thuringiensis[18]; AA414-610 is a peptide generated by protease from the venom of Gloydius halys halys[19]; I-3B antibody targeted to 15-53 residue of B-chain of fibrinogen[20]; BR42-A43 is a peptide bond that is cleaved by protease II from the venom of Echis multisquamatis[21]; B15-118 is a synthetic peptide[22]; II-4d antibody targeted to 86-240 residue of -chain of fibrinogen[23]; 69-77 is a synthetic peptide[7]; III-3B antibody targeted to 155-160 residue of B-chain of fibrinogen[24].…”