Inhibition of fibrillation of human γ
            <scp>d</scp>
            -crystallin by a flavonoid morin

Rana, Shiwani; Ghosh, Kalyan Sundar

doi:10.1080/07391102.2020.1775701

Cited by 4 publications

(3 citation statements)

References 58 publications

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“…Several studies by others have reported the existence of small molecules that bind to γ-crystallins, including lanosterol ( 59 ), cochineal carmine ( 60 ), ortho-Vanilin ( 61 ), quercetin ( 62 ), hesperetin ( 63 ), and sodium citrate ( 64 ). Moreover, small molecules with either demonstrated potential anticataract properties involving antiaggregation activity against crystallins include rosemarinic acid ( 65 ), morin ( 66 , 67 ), 25-hydrocholesterol ( 68 ), epigallocatechin gallate ( 69 ), and myoinositol ( 70 ). Among these compounds, those that were included in the screen included epigallocatechin gallate, which improved aggregation, whereas morin, lanosterol, hesperetin, and carmine had no impact.…”

Section: Discussionmentioning

confidence: 99%

α-Crystallin chaperone mimetic drugs inhibit lens γ-crystallin aggregation: Potential role for cataract prevention

Islam¹,

Michael²,

Frank³

et al. 2022

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

α-Crystallin chaperone mimetic drugs inhibit lens γ-crystallin aggregation: Potential role for cataract prevention

Islam¹,

Michael²,

Frank³

et al. 2022

Journal of Biological Chemistry

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“…18,19 Several groups previously reported that various small molecules can prevent γD-crystallin aggregation in vitro. 20,21 However, only a few have shown potential to be tried in clinical trials. In therapeutic treatment, polyphenols, which are secondary metabolites obtained from plants, have gained tremendous attention owing to their unique mode of action.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The best possible option could be to have a drug molecule preventing or delaying the aggregation of the protein. It is a well-known fact that small molecules/peptides can effectively delay and/or inhibit amyloid aggregation in other amyloid diseases. , Several groups previously reported that various small molecules can prevent γD-crystallin aggregation in vitro. , However, only a few have shown potential to be tried in clinical trials. In therapeutic treatment, polyphenols, which are secondary metabolites obtained from plants, have gained tremendous attention owing to their unique mode of action .…”

Section: Introductionmentioning

confidence: 99%

Nano-Formulation of Antioxidants as Effective Inhibitors of γD-Crystallin Aggregation

et al. 2023

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The aggregation of crystallin proteins is related to cataracts and age-related macular degeneration. Apart from surgical replacement of the cataract lens, no other alternative treatment is available till date for this ailment. In the current work, we carried out an in-depth investigation of the effect of polyphenol-loaded nano-formulations on the aggregation of γD-crystallin. At first, the protein was allowed to form amorphous aggregates under denaturing conditions. Several polyphenols were then tried to inhibit the aggregation of the protein. Among the polyphenols tested, resveratrol and quercetin were found to be the most effective. Since polyphenols are prone to degradation, they were encapsulated in chitosan nanoparticles in order to provide ambient conditions for them to function effectively. The loading efficiency and polyphenol release kinetics were subsequently tested. Finally, the efficacy of resveratrol/quercetin-loaded chitosan nano-particles as inhibitors of γD-crystallin aggregation was confirmed in a series of experiments demonstrating the potency of the system in the prospective therapeutic intervention of eye ailments concerning self-assembly of γD-crystallin proteins.

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Multispectroscopic studies on the molecular interactions between bovine γ‐globulin and borohydride‐capped silver nanoparticles

et al. 2022

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Interactions between bovine γ-globulin (BGG) and borohydride-capped silver nanoparticles (BAgNPs) were studied using dynamic light scattering (DLS) and spectroscopic techniques such as UV-vis spectroscopy, fluorescence, and circular dichroism. The results were compared with earlier reported interactions between γ-globulin and citrate-coated AgNPs (CAgNPs). BAgNPs were synthesized and characterized. Irrespective of the coating onAgNPs, nanoparticles had formed ground-state complexes with the protein. CAgNPs, as well as BAgNPs had caused static quenching of tryptophan (Trp) fluorescence of the protein. The change in the capping agent from citrate to borohydride weakened the binding of nanoparticles with the protein. But the same change in capping agent had increased the fluorescence quenching efficiency of AgNPs. Hydrogen bonding and van der Waals interactions were involved in BGG-BAgNPs complex similar to the CAgNPs complex with γ-globulin. Polarity of the Trp microenvironment in BGG was not altered using BAgNPs as opposed to CAgNPs, as supported using synchronous and three-dimensional fluorescence. Resonance light scattering experiments also suggested nano-bio conjugation. Far-UV and near-UV circular dichroism (CD) spectra respectively pointed towards changes in the secondary and tertiary structure of BGG by BAgNPs, which was not observed for CAgNPs.

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Inhibition of fibrillation of human γ d -crystallin by a flavonoid morin

Cited by 4 publications

References 58 publications

α-Crystallin chaperone mimetic drugs inhibit lens γ-crystallin aggregation: Potential role for cataract prevention

α-Crystallin chaperone mimetic drugs inhibit lens γ-crystallin aggregation: Potential role for cataract prevention

Nano-Formulation of Antioxidants as Effective Inhibitors of γD-Crystallin Aggregation

Multispectroscopic studies on the molecular interactions between bovine γ‐globulin and borohydride‐capped silver nanoparticles

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