Inhibition of FcεRI-dependent mediator release and calcium flux from human mast cells by sialic acid–binding immunoglobulin-like lectin 8 engagement

Yokoi, Hidenori; Choi, Oksoon Hong; Hubbard, Walter C.; Lee, Hyun Sil; Canning, Brendan J.; Lee, Hyun H.; Ryu, Seung Duk; Gunten, Stephan von; Bickel, Carol A.; Hudson, Sherry A.; MacGlashan, Donald W.; Bochner, Bruce S.

doi:10.1016/j.jaci.2007.10.004

Cited by 139 publications

(128 citation statements)

References 32 publications

(47 reference statements)

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“…Siglec-8 is also expressed at low levels on human mast cells and basophils. Yokoi et al (2008) observed that specific antibody-mediated cross-linking of Siglec-8 inhibited FcεRI-dependent histamine and prostaglandin D2 release, as well as Ca 2+ flux, and that anti-IgE evoked contractions of human bronchial rings. The most prominent function of macrophages in inflammatory responses is removal of infiltrating apoptotic cells.…”

Section: Discussionmentioning

confidence: 94%

Expression and preliminary functional analysis of Siglec-F on mouse macrophages

Feng

Mao

2012

J. Zhejiang Univ. Sci. B

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Section: Discussionmentioning

confidence: 94%

Expression and preliminary functional analysis of Siglec-F on mouse macrophages

Feng

Mao

2012

J. Zhejiang Univ. Sci. B

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“…However, we cannot exclude a direct action of GMP on mast cell activation, as we demonstrated that GMP significantly inhibits histamine secretion from in vitro allergen-activated mast cells. It has been reported that mast cells express different sialic acid-binding immunoglobulin-type lectin receptors that can inhibit IgE degranulation and histamine release [46]. As GMP is a highly sialylated peptide [4] and can be detected in the bloodstream in significant amounts after ingestion [47], its modulatory action on mast cells may also be induced directly through sialic acid-binding immunoglobulin-type lectin receptors.…”

Section: Discussionmentioning

confidence: 99%

Novel Mechanisms Underlying the Therapeutic Effect of Glycomacropeptide on Allergy: Change in Gut Microbiota, Upregulation of TGF-β, and Inhibition of Mast Cells

Jiménez¹,

Cervantes‐García²,

Muñoz³

et al. 2016

Int Arch Allergy Immunol

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Background: The prevalence of allergic diseases is globally increasing. We have previously described that glycomacropeptide (GMP), a bioactive milk peptide, has therapeutic value in experimental models of skin hypersensitivity, anaphylaxis, and asthma, as it prevents an excessive T helper type 2 cell immune response. The aim of this study was to analyze the effect of GMP on key elements directly involved in the development or control of allergy, in order to improve the precise knowledge about its mechanism of action. Methods: Rats were systemically sensitized with ovalbumin and orally treated with GMP. Levels of Lactobacillus, Bifidobacterium, and Bacteroides were analyzed in their feces. Splenocytes were isolated and the production of transforming growth factor (TGF)-β by allergens was measured. Intradermal skin reactions were developed to evaluate in vivo activation of mast cells. Peritoneal mast cells were isolated and activated by the allergen, and histamine secretion was determined. Results: GMP administration increased the amount of intestinal Lactobacillus and Bifidobacterium of allergen-sensitized animals after 3 days of treatment. The increase in Bacteroides was also significant, but only after 17 days of GMP administration. Ten days after treatment cessation, Lactobacillus and Bacteroides were still elevated. GMP intake also elevated the production of TGF-β in the splenocytes of sensitized animals. In addition, treatment with GMP attenuated mast cell activation by the allergen and inhibited histamine secretion, without affecting the number of mast cells. Conclusions: The prebiotic action of GMP on allergy-protective microbiota, an increase in TGF-β production, and a reduction in mast cell response to allergens are novel mechanisms that explain the antiallergic activity of GMP.

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“…Human embryonic kidney 293 (HEK 293) cell stable transfectants expressing levels of Siglec-8 similar to levels found on eosinophils were prepared and maintained as described previously (Kikly et al, 2000;Yokoi et al, 2008).…”

Section: Methodsmentioning

confidence: 99%