Inhibition of Experimental Angiogenesis of Cornea by Various Doses of Doxycycline and Combination of Triamcinolone Acetonide With Low-molecular-weight Heparin and Doxycycline

Abstract

Topically administered combinations of triamcinolone acetonide plus LMWH or triamcinolone acetonide plus doxycycline had effects that contributed to efficient suppression of corneal neovascularization; these drugs were ineffective at similar concentrations used alone. Topically administered 2% (pH neutralized) doxycycline has antiangiogenic effects, which contributed to significant suppression on corneal neovascularization. This drug may be therapeutically beneficial in treatment of corneal neovascularization in clinical trials.

The effect of doxycycline temperature-sensitive hydrogel on inhibiting the corneal neovascularization induced by BFGF in rats

“…Our study further supported the existing data showing that doxycycline could inhibit corneal NV [24,25]. Furthermore, our study showed that the ideal concentration of doxycycline to inhibit rats corneal NV might be 0.05% and 0.1%.…”

“…Furthermore, our study showed that the ideal concentration of doxycycline to inhibit rats corneal NV might be 0.05% and 0.1%. This was different from what Sanders and others reported that 2% topical doxycycline neutralized solution was the recommendation for the treatment of corneal NV [25]. Two factors may cause this difference.…”

“…Acidic doxycycline solution showed better stability than neutralized solution, but it was still difficult to preserve for months. Furthermore, in the ocular neutralized microenvironment, acidic doxycycline solution caused strong rat eye irritation (data not shown) and may have slightly decreased the efficacy of doxycycline [25]. Therefore, its clinical use was limited for the topical treatment of corneal diseases.…”

“…Our previous study showed that oral doxycycline was effective in inhibiting corneal NV [24]. Sanders and others also examined the efficacy of topical doxycycline solution in the treatment of corneal NV, and reported that 2% neutralized doxycycline solution could effectively inhibit corneal NV in a rat chemical burn model [25].…”

The experience of “Hyloparin” use in clinical practice

“…Уже тогда было замечено положительное влияние препарата на микроцир-куляцию, способность к регенерации тканей, его противоотечный эффект [10]. А в сочетании с ГКС, доксициклином или аргон-лазерной коагуляцией гепарин уменьшал рост новообразованных сосудов роговицы [7,33,34,37,51]. Несмотря на то, что в общей медицине гепарин известен, прежде всего, как прямой антикоагулянт, его биологическая роль намного шире.…”