Inhibition of ethylene‐induced cellular senescence symptoms by 1‐methylcyclopropene, a new inhibitor of ethylene action

Abstract

Ethylene is known to accelerate flower senescence, but the sequence of events that links its interaction with the tissue and the final senescence symptoms is still obscure. Recently, 1‐methylcyclopropene (1‐MCP) was found to inhibit ethylene‐induced wilting in flowers. This work was carried out in order to investigate the effects of 1‐MCP on cellular senescence symptoms in petunia flowers following expossure to ethylene. Cut petunia (Petunia hybrida) flowers that were exposed to ethylene for 12 h at concentrations of 1–12 ppm wilted sooner than their untreated counterparts. This effect was abolished by a 6‐h pre‐treatment with 1‐MCP. Immediately following the ethylene treatment, decreases in petal fresh weight and total protein content were measured, along with higher electrolyte leakage, and lower membrane lipid fluidity and protein content. When applied alone, 1‐MCP had relatively little impact on these parameters. However, when the flowers were treated with 1‐MCP prior to the ethylene treatment, ethylene had no effect. These results indicate that while ethylenes effects on wilting were obvious 3 days after the treatment, cellular parameters were affected already at the end of the treatment. Since 1‐MCP repressed these early ethylene effects, it was concluded that it interferes with ethylene action in petunia flowers at a rather early stage, long before apparent wilting.

Flower bud opening and senescence in roses (Rosa hybrida L.)

“…First, those like carnation and petunia where senescence is regulated by the increased amount of ethylene production either with ageing or following pollination (Serek et al 1995). Second, like cyclamen, only became sensitive to ethylene and produce increased amount of the hormone when they are pollinated (Halevy et al 1984).…”

Role of ethylene receptors during senescence and ripening in horticultural crops

“…1-alkyl-cyclopropenes and AgNO 3 are the most prominently used ethylene antagonists. [17][18][19][20][21][22] Another class of strained alkenes, cyclobutenes and trans cyclooctene have recently been brought into picture because of their greater convenience in handling and administration. 23 Kazemi et al (2011) 24 reviewed the effect of various chemicals at different concentrations on Gebrera cut flowers.…”

Transcriptomic Analysis on the Regulation of Tomato Ripening by the Ethylene Inhibitor 1-methylcyclopropene

“…There are six known receptors in tomato (Klee & Tieman, 2002). Previous studies suggested that 1-MCP inhibited ethylene function by competing with ethylene receptors (Sisler, 2006;Serek et al, 1995). A model was proposed for 1-MCP action.…”