Inhibition of enzymatic hydrolysis of pretreated corn stover and sugar cane straw by laccases

Rocha-Martín, Javier; Martínez-Bernal, Claudio; Zamorano, Laura; Reyes-Sosa, Francisco M.; García, Bruno Díez

doi:10.1016/j.procbio.2018.01.021

Cited by 16 publications

(17 citation statements)

References 26 publications

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“…Researchers have shown that laccases can work more efficiently in combination with hemicellulases for lignocellulose saccharification. However, Rocha-Martín et al [ 115 ], reported that supplementation of laccase in enzymatic hydrolysis showed contradictory results, depending on pretreatment type and biomass used. The addition of laccase to softwood hydrolysis resulted in high glucose yield, while a negative effect was obtained for hardwood hydrolysis.…”

Section: Application Of Lignin-active Enzymesmentioning

confidence: 99%

Deconstruction of Lignin: From Enzymes to Microorganisms

et al. 2021

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Lignocellulosic residues are low-cost abundant feedstocks that can be used for industrial applications. However, their recalcitrance currently makes lignocellulose use limited. In natural environments, microbial communities can completely deconstruct lignocellulose by synergistic action of a set of enzymes and proteins. Microbial degradation of lignin by fungi, important lignin degraders in nature, has been intensively studied. More recently, bacteria have also been described as able to break down lignin, and to have a central role in recycling this plant polymer. Nevertheless, bacterial deconstruction of lignin has not been fully elucidated yet. Direct analysis of environmental samples using metagenomics, metatranscriptomics, and metaproteomics approaches is a powerful strategy to describe/discover enzymes, metabolic pathways, and microorganisms involved in lignin breakdown. Indeed, the use of these complementary techniques leads to a better understanding of the composition, function, and dynamics of microbial communities involved in lignin deconstruction. We focus on omics approaches and their contribution to the discovery of new enzymes and reactions that impact the development of lignin-based bioprocesses.

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Section: Application Of Lignin-active Enzymesmentioning

confidence: 99%

Deconstruction of Lignin: From Enzymes to Microorganisms

et al. 2021

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“…Laccase can catalyze the oxidation of a variety of aromatic compounds (preferably phenolic compounds) [1,2] and has been widely employed for delignification, plant fiber derivatization, textile dye or stain bleaching, and contaminated water or soil detoxification [3][4][5][6]. However, the industrial applications of laccase are still seriously limited owing to their poor stability and high cost [7].…”

Section: Introductionmentioning

confidence: 99%

An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity

Zhang

Chen

et al. 2018

Catalysts

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In this work, laccase from Trametes versicolor pretreated with copper ion solution was entrapped in copper alginate beads. The presence of laccase in copper alginate beads was verified by Fourier transform infrared (FTIR) spectroscopy. The alginate concentration used was optimized based on the specific activity and immobilization yield. After entrapment, laccase presents perfect pH stability and thermal stability with 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) as the substrate. Moreover, laccase in copper alginate beads exhibits good reusability during continuous batch operation for removing 2,4-dichlorophenol. More importantly, owing to the coupled effect of copper ion activation and copper alginate entrapment, the entrapped laccase shows a 3.0-fold and a 2.4-fold increase in specific activity and 2,4-DCP degradation rate compared with that of free laccase, respectively.

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“…Sugars can be obtained from cellulose and hemicellulose in a process called saccharification, which involves enzymatic hydrolysis by a set of enzymes generically known as cellulases that work synergistically to produce monomeric sugars ( € Ohgren et al, 2007;Alvarez et al, 2016). Glucose (about 57-70%) is the major product that results from the hydrolysis, followed by xylose (about 9-23%), and other minority sugars such as arabinose, rhamnose and galactose (van Maris et al, 2006;Rocha-Mart ın et al, 2018; see Table S1 for data from Rocha-Mart ın et al, 2018). However, many industrially relevant microorganisms only ferment glucose and leave the other sugars untransformed.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of aromatic amino acids from 2G lignocellulosic substrates

Godoy

García‐Franco

Recio

et al. 2021

Microbial Biotechnology

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Pseudomonas putida is a highly solvent-resistant microorganism and useful chassis for the production of value-added compounds from lignocellulosic residues, in particular aromatic compounds that are made from phenylalanine. The use of these agricultural residues requires a two-step treatment to release the components of the polysaccharides of cellulose and hemicellulose as monomeric sugars, the most abundant monomers being glucose and xylose. Pan-genomic studies have shown that Pseudomonas putida metabolizes glucose through three convergent pathways to yield 6-phosphogluconate and subsequently metabolizes it through the Entner-Doudoroff pathway, but the strains do not degrade xylose. The valorization of both sugars is critical from the point of view of economic viability of the process. For this reason, a P. putida strain was endowed with the ability to metabolize xylose via the xylose isomerase pathway, by incorporating heterologous catabolic genes that convert this C5 sugar into intermediates of the pentose phosphate cycle. In addition, the open reading frame T1E_2822, encoding glucose dehydrogenase, was knocked-out to avoid the production of the dead-end product xylonate. We generated a set of DOT-T1E-derived strains that metabolized glucose and xylose simultaneously in culture medium and that reached high cell density with generation times of around 100 min with glucose and around 300 min with xylose. The strains grew in 2G hydrolysates from diluted acid and steam explosion pretreated corn stover and sugarcane straw. During growth, the strains metabolized > 98% of glucose, > 96% xylose and > 85% acetic acid. In 2G hydrolysates P. putida 5PL, a DOT-T1E derivative strain that carries up to five independent mutations to avoid phenylalanine metabolism, accumulated this amino acid in the medium. We constructed P. putida 5PLDgcd (xylABE) that produced up to 250 mg l À1 of phenylalanine when grown in 2G pretreated corn stover or sugarcane straw. These results support as a proof of concept the potential of P. putida as a chassis for 2G processes.

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Inhibition of enzymatic hydrolysis of pretreated corn stover and sugar cane straw by laccases

Cited by 16 publications

References 26 publications

Deconstruction of Lignin: From Enzymes to Microorganisms

Deconstruction of Lignin: From Enzymes to Microorganisms

An Improved Method to Encapsulate Laccase from Trametes versicolor with Enhanced Stability and Catalytic Activity

Synthesis of aromatic amino acids from 2G lignocellulosic substrates

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