Inhibition of embryonic development and fertilization in broadcast spawning marine invertebrates by water soluble diatom extracts and the diatom toxin 2-trans,4-trans decadienal

Caldwell, Gary S.; Olive, P. J. W.; Bentley, M. G.

doi:10.1016/s0166-445x(01)00277-6

Cited by 78 publications

(67 citation statements)

References 53 publications

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“…While Miralto et al (1999) speculated as to the apparent lack of detoxification systems in copepods, Ianora et al (2003) showed that the same diatom species could induce different effects in different copepod species. Benthic invertebrates have revealed pronounced species-specific variability in the sensitivity of their embryos to aldehyde toxicity (Caldwell et al 2002). No inhibitory effects on reproductive and population growth parameters were observed following ingestion of the benthic diatom Nitzschia closterium by the harpacticoid copepod Tisbe biminiensis (Pinto et al 2001).…”

Section: Main Contributors: G S Caldwell and J T Turnermentioning

confidence: 99%

Colloquium on diatom-copepod interactions

Paffenhöfer¹,

Ianora²,

Miralto³

et al. 2005

Mar. Ecol. Prog. Ser.

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From 3 to 6 November 2002, a colloquium was convened at the Benthos Laboratory of the Stazione Zoologica Anton Dohrn on Ischia, Italy, with the goal of evaluating the present status of the effects of diatoms on their main consumers, planktonic copepods, and to develop future research strategies to enhance our understanding of such interactions. These included (1) toxic effects of diatom metabolites on copepods, particularly reproduction, and (2) nutritional effects of diatoms on juvenile to adult copepods. Key issues involved in the impact of diatoms on the dynamics of natural plankton communities in situ were also addressed. During the plenary session, the most recent advances on this topic were presented. The plenary session was followed by 3 working groups on (1) production of aldehydes by phytoplankton, (2) toxic and nutritional effects of diatoms on zooplankton, and (3) the chemistry of diatom defense, as well as of their nutritional quality. These working groups focused on suggesting future research needs for the different topics. As a result, several recommendations were outlined, including experimental studies. It became evident that interdisciplinary efforts are needed, involving chemists, oceanographers and experimentalists, since many of the biological observations under controlled conditions and in situ require an integrated approach, including chemical causation. Extensive field observations based on common protocols are also recommended for investigation of the intrinsic variability of such effects and their environmental controls. Laboratory experiments are seen to be essential for the full understanding of environmentally occurring processes.

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Section: Main Contributors: G S Caldwell and J T Turnermentioning

confidence: 99%

Colloquium on diatom-copepod interactions

Paffenhöfer¹,

Ianora²,

Miralto³

et al. 2005

Mar. Ecol. Prog. Ser.

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“…This may explain why the persistence of this compound is short but extended at lower temperatures. The aldehydes type α -β were described as toxic for marine organism, principally invertebrates e.g: Nereis virens (Lewis et al, 2004), Calanus helgolandicus , Temora stylifera (Ceballos & Ianora, 2003), Asteria rubens (Caldwell et al, 2002), stopping reproduction process. These compounds also showed to be toxic for rats (Adams et al, 2008) and human lung cells (Chang & Lin, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…These lice could suffer an alteration during its embryogenesis which explains their further impossibility to develop the frontal filament. Several authors have described deformities or inhibitions in the development of the limbs of copepods embryos and larvaes exposed to different doses of this unsaturated aldehyde (Caldwell et al, 2002;Romano et al, 2003;Ianora et al, 2004;Lewis et al, 2004;Taylor et al, 2005).…”

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confidence: 99%

Effect of polyunsatured aldehyde (A3) as an antiparasitary ingredient of Caligus rogercresseyi in the feed of Atlantic salmon, Salmo salar

Troncoso¹,

González²,

Pino³

et al. 2011

lajar

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ABSTRACT. Some polyunsaturated aldehydes (PUAs) such as 2-trans, 4-trans decadenial (A3) have a detrimental effect on the development of copepod sharmful to the salmon industrysuch as Caligus rogercresseyi (sea lice). The purpose of this study was to evaluate the potential use of A3 as a salmon feed ingredient in order to reduce reproduction of Caligus rogercresseyi in infested Atlantic salmon (Salmo salar). The toxicity of A3 was assessed histopathologically for 7 days, using intra-peritoneal injections of different doses (0, 0.24, 0.47, 2.37, 11.86, and 23.71 mg kg -1 ) in brain, intestine, skin, liver, and muscletissues of Salmosalar at the end of each treatment. The effect of A3 on sea lice was evaluated using 250 Atlantic salmon in an open-flow system of seawater (~13°C). The effect of the dosage in the fish diet was evaluated at two levels (9 mg kg -1 , 18 mg kg -1 ) and considering a control (without A3) for 4, 8, and 12 days, once the sea lice had reached maturity. As a complement, the persistent effect of diluted A3 in sea water (0.5 g L -1 ) was evaluated at 10, 12, and 14°C for 0, 1, 3, 6, 10, and 15 days; and the maximum persistence was found at 10 days at 10°C. The results showed that the dosages over 0.47 mg kg -1 had no toxic effect on Atlantic salmon, but induced a detrimental effect on Caligus rogercresseyi (reduction of 15% of mature females with a dose of 18 mg kg -1 ), which could be attributed to alterations in the embryonic development of the sea lice. A3 is a potential supplement in the diet of salmon. However, studies ofits mechanism of action should be undertaken prior to its use. Keywords: Caligus rogercresseyi, sea lice, PUA, reproduction, A3, infestation, Salmo salar, aquaculture, Chile.Efecto del aldehído poli-insaturado (A3) como ingrediente antiparasitario de Caligus rogercresseyi en la alimentación de salmón del atlántico, Salmo salar RESUMEN. Algunos aldehídos poli-insaturados (PUAs) tales como 2-trans, 4-trans decadenal (A3) tienen efecto perjudicial para el desarrollo de copépodos perjudiciales para la industria del salmón, tales como Caligus rogercresseyi (piojo de mar). El objetivo del presente estudio fue evaluar el uso potencial de A3 en alimentación de salmones, de forma de reducir la reproducción de Caligus rogercresseyi, en salmón del Atlántico (Salmo salar) infestado. Se evaluó en forma histopatológica la toxicidad de A3 durante siete días, a través de distintas dosis (0, 0,24, 0,47, 2,37, 11,86 y 23,71 mg kg -1 ) inyectadas por vía intra-peritoneal en Salmo salar, en tejidos de cerebro, intestino, piel, hígado y músculo, al final de cada tratamiento, mientras que el efecto de A3 contra los piojos de mar fue evaluado usando 250 salmones del Atlántico en un sistema de flujo abierto de agua de mar (~13°C). El efecto de la dosis en la dieta de los peces se evaluó a dos niveles (9 mg kg -1 y 18 mg kg -1 ) y con una muestra control (sin A3), por 4, 8 y 12 días, una vez alcanzado el estado maduro del piojo de mar. En forma complementaria, se evaluó el efecto de la persis...

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“…Concentration ranges of test substances were selected according to His et al (1999), King and Riddle (2001) and Fernández and Beiras (2001) for copper, and for TBP, Roepke et al (2005) and Kiyomoto et al (2006) for bisphenol A and Steevens et al (1999) and Pillai et al (2003) for phenanthrene. Eggs and sperm were obtained as described above but eggs were released in general purpose medium 2 (GP 2: 360 mM NaCl, 24.8 mM Na 2 SO 4 , 8.2 mM KCl, 0.74 mM KBr, 0.09 mM Na 2 B 4 O 7 ×10H 2 0, 46.7 mM MgCl 2 ×6H 2 O, 11.9 CaCl 2 ×2H 2 O, 0.08 mM SrCl 2 ×6H 2 O, 2.02 mM NaHCO 3 ) (instead of ASW) which had been aerated for 24 h (U.S. EPA, 1993;Caldwell et al, 2002). During fertilization assays it is essential to keep the concentrations of sperm and eggs constant in the different treatments and replicates.…”

Section: In Vitro Fertilization Assaysmentioning

confidence: 99%

“…Four replicate incubations were run per treatment. After 60 min, 10 µL of sperm suspension in GP2 were added to give a final sperm concentration of 2.5 × 10 6 mL − 1 (Caldwell et al, 2002). Sperm had been allowed to activate in GP2 approximately 10 min prior to use.…”

Section: In Vitro Fertilization Assaysmentioning

confidence: 99%

Measuring Ca2+-signalling at fertilization in the sea urchin Psammechinus miliaris: Alterations of this Ca2+-signal by copper and 2,4,6-tribromophenol

Schäfer

Bickmeyer

Köehler

2009

Comparative Biochemistry and Physiology Part C: Toxicology &

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During fertilization, eggs undergo a temporary rise in the intracellular concentration of free Ca 2+ ions. Using the membrane permeable acetoxymethylester of the fluorescent calcium indicator dye Fura-2, Fura-2 AM, the Ca 2+ -signal at fertilization was not detectable in eggs of the sea urchin Psammechinus miliaris. However, after treatment of the eggs with Fura-2 AM in combination with MK571, an inhibitor for multidrug resistance associated proteins, clear Ca 2+-signals at fertilization could be measured without microinjection of the dye. We used this methodology to detect possible alterations of Ca 2+ -signalling at fertilization by exposure of eggs to environmental pollutants. For this purpose, the heavy metal copper, the bromophenol 2,4,6-tribromophenol, the organic compound bisphenol A and the polycyclic aromatic hydrocarbon phenanthrene were tested for their potential to inhibit fertilization success of P. miliaris. Copper and 2,4,6-tribromophenol showed a dose-dependent effect on fertilization rates of P. miliaris and significantly inhibited fertilization at 6.3 µM Cu 2+ and 1 µM 2,4,6-tribromphenol. Bisphenol A significantly inhibited fertilization success at 438 µM while phenanthrene had no effect up to 56 µM. 6.3 µM copper and 100 µM 2,4,6-tribromophenol significantly increased the Ca 2+ -signal at fertilization. This alteration may contribute to the reduced fertilization rates of P. miliaris after exposure to copper and 2,4,6-tribromophenol.

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Inhibition of embryonic development and fertilization in broadcast spawning marine invertebrates by water soluble diatom extracts and the diatom toxin 2-trans,4-trans decadienal

Cited by 78 publications

References 53 publications

Colloquium on diatom-copepod interactions

Colloquium on diatom-copepod interactions

Effect of polyunsatured aldehyde (A3) as an antiparasitary ingredient of Caligus rogercresseyi in the feed of Atlantic salmon, Salmo salar

Measuring Ca2+-signalling at fertilization in the sea urchin Psammechinus miliaris: Alterations of this Ca2+-signal by copper and 2,4,6-tribromophenol

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