Inhibition of E-rosette formation by two iron salts

Sousa, Maria de; Nishiya, Koji

doi:10.1016/0008-8749(78)90048-5

Cited by 35 publications

(4 citation statements)

References 15 publications

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“…Although relatively few studies have investigated the relationship between iron excess and immune function in vivo there is general agreement that increased concentrations of iron are deleterious toT lymphocyte function, natural killer cell (NK) activity and macrophage cytotoxicity./n vitro studies have shown that addition of increasing concentrations of iron inhibit E-rosette formation (44)(45)(46), mitogen-induced proliferation of lymphocytes (PHA and concanavalin A) (47,48), and the mixed lymphocyte reaction (45) and NK cell activity (49).…”

Section: Iron Excess and Immunitymentioning

confidence: 99%

Iron and Immunity

Farthing

1989

Acta Paediatrica

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Farthing, M. J. G. (Department of Gastroenterology, St Bartholomew's Hospital, London, ECIA 7BE, UK). Iron and immunity. Review of animal and human studies concerning the impact of iron deficiency on immune function in vivo indicates that in many instances there is no firm consensus of opinion as to the relationship between iron status and immunity. One major problem with almost all human studies is that other micro‐ and macronutrient deficiencies are inadequately controlled for and thus it is often unclear as to whether reported abnormalities of immune function can be attributed specifically to iron deficiency. Even when abnormalities of immune function have been detected it is often uncertain as to the biological and clinical relevance that these may have for the host. Within these restraints the available studies suggest that iron deficiency may at least contribute to impaired T lymphocyte function as judged by DTH responses in skin and impaired mitogen‐induced proliferation. As in protein energy malnutrition, humoral immunity is largely spared in humans, the balance of evidence suggesting that immunoglobulin production and function is normal, as are serum concentrations of complement. The only other abnormality of non‐specific immunity which has been reported consistently to be abnormal is that of reduced bactericidal activity of polymorphonuclear leucocytes. The clinical relevance of these abnormalities remains to be established. There is, however, no evidence to suggest that individuals with iron deficiency suffer the devastating infective complications of the well defined immunodeficiency syndromes either congenital or acquired. It seems likely therefore that despite the fundamental importance of iron in maintaining the integrity of immune function, humans can tolerate the extremes of deficiency and excess and survive in a relatively healthy state.

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Section: Iron Excess and Immunitymentioning

confidence: 99%

Iron and Immunity

Farthing

1989

Acta Paediatrica

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“…Mounting evidence derived from experiments testing the in vitro effects of metals on immune responses (Hartmut et al 1989), indicates that some metals can effectively modulate expression of T lymphocyte surface molecules and T cell responses (Treagan 1975, de Sousa & Nishiya 1978, Nishiya et al 1980, Bryan et al 1981, Warner & Lawrence 1986, Santos & de Sousa 1994, Arosa & de Sousa 1995. In this study an in vitro method was developed to determine whether T cell proliferation was affected in the presence of two metal alloy discs.…”

Section: Introductionmentioning

confidence: 99%

Cobalt-chromium-molybdenum but not titanium-6aluminium-4vanadium alloy discs inhibit human t cell activation in vitro

Faleiro¹,

Godinho²,

Reus³

et al. 1996

Biometals

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This study describes the effect of the presence of cobalt-chromium-molybdenum (CoCrMo) and titanium-6aluminium-4vanadium (Ti6AL4V) disc samples on the CD3-mediated in vitro response of human peripheral blood T lymphocytes. Lymphocyte proliferation in the presence and absence of these metal alloy discs was measured by [3H]thymidine incorporation. Inhibition of lymphocyte proliferation was observed in the presence of CoCrMo disc samples. In contrast, the presence of the Ti6AL4V metal alloy discs had no effect on T cell proliferation. Ultrastructural studies using scanning electron microscopy revealed that the differences in the number of blast cells on uncoated CoCrMo and Ti6AL4V discs from a 4 day culture were consistent with the results observed in the proliferation experiments, i.e. fewer blast cells were seen on the CoCrMo than on the Ti6AL4V discs. In addition, a quantitative analysis of trace elements using total reflection X-ray fluorescence spectrometry in supernatants from 68 h in vitro cultures containing Ti6AL4V or CoCrMo disc samples was performed, revealing differences in the relative metal concentrations in the culture conditions tested. These differences point to the presence of cobalt in the supernatants as a possible determining factor of the inhibition observed. Because cell viability did not appear to change, a more complex mechanism involving the interaction of metals with T lymphocytes may account for the results obtained.

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“…Low molecular weight iron complexes have been shown to inhibit the expression ofsheep red eell receptors on human T lymphocytes (6). In addition, iron has been implieated as an inhibitory agent in the mixed lymphocyte eultLire response (7) and in mitogeninduced proliferation (8,9).…”

Section: Introddcnonmentioning

confidence: 99%

Clonal analysis of the effect of iron on human cytotoxic and proliferating T lymphocytes

Rudd

Good

Chapman

et al. 1990

Immunology & Cell Biology

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SummaryThe immunoregulatory effect ofnon-transferrin-bound iron (Fe'"'') on the proliferative and cytotoxic responses of normal human T lymphocytes was studied using a sensitive limit-dilution teehnique capable of detecting the responses of individual lymphocytes. Iron, present in the form of ferric citrate at concentrations from 003 to I'0 mmol/L. significantly reduced the cloning frequency of peripheral blood T lymphocytes. The eliect ol'iron appeared, however, to be targeted to individual clones in that some clones thai did grow in the presence of iron achieved a normal rateof prolileralion. Thus, iron was not non-spccifically toxic. At these same concentrations ferric cilrate also produced significant reductions in Ihe cloning frequency of CD4 f CD8-precursor T lymphocytes. Reductions in the response of T lymphocyte precursors capable of cytotoxic activity occurred in the presence of ferric cilrate from ()•! lo I •() mmol/L. These data support the hypothesis that non-translerrin-bound iron has an immunoregulatory role in ccli-mcdiated immunity.

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Inhibition of E-rosette formation by two iron salts

Cited by 35 publications

References 15 publications

Iron and Immunity

Iron and Immunity

Cobalt-chromium-molybdenum but not titanium-6aluminium-4vanadium alloy discs inhibit human t cell activation in vitro

Clonal analysis of the effect of iron on human cytotoxic and proliferating T lymphocytes

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