Inhibition of DNA ejection from bacteriophage by Mg+2 counterions

Lee, SeIl; Tran, C. V.; Nguyen, Toan T.

doi:10.1063/1.3569133

Cited by 9 publications

(8 citation statements)

References 34 publications

(58 reference statements)

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“…Figure 8 gives the short-range attraction among DNA molecules to be −0.008k B T/base. This is about twice as large as the fitted value obtained from the viral DNA ejection experiments in Section 4 [40]. There are many factors that leads to this quantitative discrepancy.…”

Section: Counterion Mediated Dna-dna Interactions and The Dna Packagimentioning

confidence: 59%

Strongly correlated electrostatics of viral genome packaging

Nguyen

2013

J Biol Phys

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The problem of viral packaging (condensation) and ejection from viral capsid in the presence of multivalent counterions is considered. Experiments show divalent counterions strongly influence the amount of DNA ejected from bacteriophage. In this paper, the strong electrostatic interactions between DNA molecules in the presence of multivalent counterions is investigated. It is shown that experiment results agree reasonably well with the phenomenon of DNA reentrant condensation. This phenomenon is known to cause DNA condensation in the presence of tri-or tetra-valent counterions. For divalent counterions, the viral capsid confinement strongly suppresses DNA configurational entropy, therefore the correlation between divalent counterions is strongly enhanced causing similar effect. Computational studies also agree well with theoretical calculations.

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Section: Counterion Mediated Dna-dna Interactions and The Dna Packagimentioning

confidence: 59%

Strongly correlated electrostatics of viral genome packaging

Nguyen

2013

J Biol Phys

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“…Indeed, DNA condensation by divalent counterions has also been observed in another environment where DNA configuration is constrained, namely the condensation of DNA in two dimensional systems [14]. For virus systems, theoretical fitting suggests that the DNA is neutralized at c Z,0 ≈ 75mM for divalent counterions, and the short−range DNA attraction at this concentration is −0.004k B T per nucleotide base [15,16].…”

Section: Introductionmentioning

confidence: 99%

DNA like-charge attraction and overcharging by divalent counterions in the presence of divalent co-ions

Nguyen

Carloni

2017

J Biol Phys

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Strongly correlated electrostatics of DNA systems has drawn the interest of many groups, especially the condensation and overcharging of DNA by multivalent counterions. By adding counterions of different valencies and shapes, one can enhance or reduce DNA overcharging. In this paper, we focus on the effect of multivalent co-ions, specifically divalent co-ions such as SO[Formula: see text]. A computational experiment of DNA condensation using Monte Carlo simulation in grand canonical ensemble is carried out where the DNA system is in equilibrium with a bulk solution containing a mixture of salt of different valency of co-ions. Compared to systems with purely monovalent co-ions, the influence of divalent co-ions shows up in multiple aspects. Divalent co-ions lead to an increase of monovalent salt in the DNA condensate. Because monovalent salts mostly participate in linear screening of electrostatic interactions in the system, more monovalent salt molecules enter the condensate leads to screening out of short-range DNA-DNA like charge attraction and weaker DNA condensation free energy. The overcharging of DNA by multivalent counterions is also reduced in the presence of divalent co-ions. Strong repulsions between DNA and divalent co-ions and among divalent co-ions themselves lead to a depletion of negative ions near the DNA surface as compared to the case without divalent co-ions. At large distances, the DNA-DNA repulsive interaction is stronger in the presence of divalent co-ions, suggesting that divalent co-ions' role is not only that of simple stronger linear screening.

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“…We are not addressing the specific binding effects 181,227 but the non-specific universal features of the polyvalent counterions. These effects have not been analyzed in detail yet, but there are some features that can be addressed without delving too deeply into the theory of strongly charged Coulomb systems.…”

Section: "Dressed Counterion" Approximation: Nonspecific Effects Of Pmentioning

confidence: 99%

Energies and pressures in viruses: contribution of nonspecific electrostatic interactions

Šiber

Božič

Podgornik

2012

Phys. Chem. Chem. Phys.

130

153

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We summarize some aspects of electrostatic interactions in the context of viruses. A simplified but, within well defined limitations, reliable approach is used to derive expressions for electrostatic energies and the corresponding osmotic pressures in single-stranded RNA viruses and double-stranded DNA bacteriophages. The two types of viruses differ crucially in the spatial distribution of their genome charge which leads to essential differences in their free energies, depending on the capsid size and total charge in a quite different fashion. Differences in the free energies are trailed by the corresponding characteristics and variations in the osmotic pressure between the inside of the virus and the external bathing solution.

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Inhibition of DNA ejection from bacteriophage by Mg+2 counterions

Cited by 9 publications

References 34 publications

Strongly correlated electrostatics of viral genome packaging

Strongly correlated electrostatics of viral genome packaging

DNA like-charge attraction and overcharging by divalent counterions in the presence of divalent co-ions

Energies and pressures in viruses: contribution of nonspecific electrostatic interactions

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