Inhibition of digestive proteases by vegetable meals in three fish species; seabream (Sparus aurata), tilapia (Oreochromis niloticus) and African sole (Solea senegalensis)

Moyano, Francisco Javier; Dı́az, Isabel; López, Manuel Díaz

doi:10.1016/s0305-0491(99)00024-3

Cited by 60 publications

(27 citation statements)

References 15 publications

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“…Similar values of trypsin activity are also shown by Robaina et al (1995) in gilthead seabream fed with control and 20% soybean meal inclusion levels. Moreover, it is interesting to note that TAP did not change significantly in rainbow trout fed with VW-added diets, contrary to those reported in seabream, tilapia, and African sole that were inhibited by vegetable meals (Moyano Lopez et al 1999).…”

Section: Digestive Enzymescontrasting

confidence: 66%

Physiological effects of natural olive oil antioxidants utilization in rainbow trout (Onchorynchus mykiss) feeding

et al. 2009

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Olive mill vegetation water (VW) is an olive oil by-product rich in polyphenols has powerful antioxidant effects. In light of the interest on the research of novel natural antioxidants to use in fish feed, the aim of this research was to use VW as a potential substitute for artificial antioxidants in rainbow trout diet as well as checking its effects on the blood chemistry and digestive organ physiology of the fish. The experimental plan was monofactorial, balanced (4 9 3) and the experimental factor considered was the fish diet. Diets were isoproteic (CP 40%) and isoenergetic (18 MJ kg -1 DM) with two inclusion levels of VW (1 and 5%: VW1 and VW5) tested against a control diet. A feeding trial was performed on quadruplicate groups of 200 fish (mean body weight: 44.2 g) fed experimental diets for 94 days. At the end of the trial, the growth performance traits were determined and sampling of blood and different tissues (brain, ovary, stomach, liver, and intestine) were carried out for haematology, endocrinology, histology, and digestive enzyme analysis. The main results of the present experimentation are that VW inclusion in rainbow trout feed slightly affects the productive traits and blood chemistry, while the histological structure of digestive organs and digestive enzyme physiology were not affected.

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Section: Digestive Enzymescontrasting

confidence: 66%

Physiological effects of natural olive oil antioxidants utilization in rainbow trout (Onchorynchus mykiss) feeding

et al. 2009

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“…8). Moyano López et al. (1999) stated that bolti digestive proteases showed great sensitivity to the protease inhibitor present in the defatted soybean because high inhibition rates (nearly 40%) were reached even with a very low concentration.…”

Section: Resultsmentioning

confidence: 99%

Purification and Characterization of an Alkaline Protease From the Viscera of Bolti Fish (Tilapia Nilotica)

El‐Beltagy¹,

El‐Adawy²,

Rahma³

et al. 2005

J Food Biochemistry

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An alkaline protease was extracted from acetone powder of the viscera of bolti fish (Tilapia nilotica) with distilled water, precipitated from the extract by 40–60% (NH4)2SO4and then dialyzed. Enzyme homogeneity studies by polyacrylamide gel electrophoresis (PAGE) illustrated that the enzyme was homogenous. Sodium dodecyl sulfate–PAGE showed a molecular weight of 23.0 kDa. The optimal pH and optimal temperature were 8.0 and 45C, respectively, with casein as a substrate. A remarkable stability was observed under alkaline conditions (pH 6.0–10.0), where more than 90% of the enzyme activity was maintained. In the acidic region (pH 2.0–6.0), the enzyme retained more than 50% of its activity. Furthermore, the enzyme retained 85.4 and 41.8% of its activity after heating at 50 and 60C for 120 min, respectively, while the relative activity after heating at 70C for 120 min was 5.25%. The alkaline enzyme lost most of its activity when incubated at 80C for 30 min. A high percentage of total enzyme activity was inhibited when the enzyme was incubated with 50 mM of either soybean trypsin inhibitor or ethylenediaminetetraacetic acid. The presence of NaCl and CaCl2at 10 mM concentration increased the enzyme activity by 6.9 and 10.6%, respectively.

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“…Furthermore, fish species can have different tolerance limits to the presence of antinutrients (Francis et al 2001). In a comparative study on Nile tilapia O. niloticus, gilthead seabream Sparus aurata, and African sole Solea senegalensis, the last had the greatest resistance of sole to protease inhibitors in defatted SBM (Moyano Lopez et al 1999). A second comparative study between Atlantic salmon and rainbow trout suggested that rainbow trout has a higher tolerance for SBM ''agents'' causing histopathological changes in the intestinal mucosa than Atlantic salmon, indicating the existence of species-specific differences even within the same family.…”

Section: Discussionmentioning

confidence: 99%

Growth performance, feed utilization, and body composition of Nile tilapia (Oreochromis niloticus L.) fed with differently heated soybean-meal-based diets

et al. 2008

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This study was undertaken in order to determine the effect of feeding heattreated, defatted soybean meal (SBM) on growth, feed utilization, and body composition of Nile tilapia (Oreochromis niloticus). A control diet (SBM 0 ) of 378 g kg -1 crude protein with 18.4 kJ g -1 gross energy was formulated, and three diets identical to the basal diet were autoclaved for 10, 20, or 30 min. The autoclaved diets were named SBM 10 , SBM 20 , and SBM 30 , respectively. Each diet treatment was applied to triplicate groups of 30 fish (2.45 ± 0.03 g) per tank (120 l). The fish were hand fed to satiation four times daily for 45 days. At the end of the feeding trial the fish fed with the SBM 30 diet had significantly (P \ 0.05) higher weight gain and protein efficiency ratio than those fed with the other diets. No feed-related mortality was observed during the whole experimental period. Heating SBM for 30 min reduced trypsin inhibitor activity (TIA) and increased apparent protein digestibility (APD), and indicated significant differences (P \ 0.05) among various treatments. No significant differences were found in carcass moisture, lipid, and ash of fish fed with different experimental diets. An increase in the body protein content of fish fed with diet SBM 30 was significantly (P \ 0.05) higher than in all other experimental groups. The results of this study seem to indicate that autoclaving the SBM for 30 min improved its nutritional value in practical feeds for Nile tilapia fingerlings.

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Inhibition of digestive proteases by vegetable meals in three fish species; seabream (Sparus aurata), tilapia (Oreochromis niloticus) and African sole (Solea senegalensis)

Cited by 60 publications

References 15 publications

Physiological effects of natural olive oil antioxidants utilization in rainbow trout (Onchorynchus mykiss) feeding

Physiological effects of natural olive oil antioxidants utilization in rainbow trout (Onchorynchus mykiss) feeding

Purification and Characterization of an Alkaline Protease From the Viscera of Bolti Fish (Tilapia Nilotica)

Growth performance, feed utilization, and body composition of Nile tilapia (Oreochromis niloticus L.) fed with differently heated soybean-meal-based diets

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