Inhibition of Dextran Edema by Proteolytic Enzymes

Abstract

517 the concentration of K+ or Na+ was 0.1 M or more. Also, the smaller amount of alkali required to adjust maleate buffer to pH 5.5 than to 7.9 or 7.0 may have augmented the inhibition reported by him at pH 5.5 because of the lower ionic strength; however, our results indicate, as do his, greater inhibition at acid pH values. The splitting of ATP by U02C12 may have had bearing upon his failure to detect inhibition at pH 7.9. This is especially likely if he used long incubations.1. It has been demonstrated that uranyl chloride ( UO2Cl2) inhibits myosin ATPase activity. The extent of inhibition is influenced by the strength of K-ion and is enhanced by calcium or magnesium. The pH value 6.6, of those tested, is most favorable for inhibition. 2 . Uranyl chloride per se Summary.causes dephosphorylation of ATP. This action is less than that caused by cerium and about equal to that of lanthanum.

Anti-Inflammatory Activity of a Proteolytic Enzyme, Prozime-10

“…It has been reported that the anti-inflamma tory activity of the proteolytic enzymes, such as a-chymotrypsin do not appear to be medi ated by stimulation of the pituitary-adrenal axis (5,25), since the anti-inflammatory effects remain after adrenalectomy. In tire present experiment, the plasma corticosterone level was markedly raised after intravenous injection of P-10.…”

“…In most investigations on the anti-inflamma tory effect of proteolytic enzymes, the preven tive effect of protease is evaluated after intra peritoneal (5,26) or subcutaneous (14) admin istration. In either case, severe inflammation occurs at the injected sites and this may be responsible for a nonspecific 'inhibitory effect' (8,18).…”

“…Proteolytic enzymes, such as trypsin (5,14), a-chymotrypsin (14,15,26) and bromelain (16,20) administered orally as enteric-coated preparations are known to be efficacious as anti-inflammatory agents. However, definite proof as to whether the enzymes given orally reach the systemic circulation is not yet avail able.…”

Activité anti-inflammatoire comparée de substances anticoagulantes. Mode d’action de la Phénylindanedione

“…Aux oedèmes généralisés déclenchés par injection de dextran (4,5,16) qui se prêtent mal à une mesure précise de l'effet pro tecteur des drogues, nous avons préféré une action phlogistique locale. Cette action est habituellement développée sur deux es pèces : le rat et le cobaye.…”

Anti-Inflammatory Mechanism of Prozime-10, a Proteolytic Enzyme

“…It has been reported that anti-inflamma tory activity of proteolytic enzymes such as trypsin [Cohen et al, 1955;Martin et al, 1954], a-chymotrypsin [Martin et al, 1954;Miecowski and Ercoli, 1956;Yokoyama et al, 1975], may not be related to the adrenal gland, since a remarkable anti-inflammatory effect has been shown in adrenalectomized rats [Cohen et al, 1955;Yamasaki et al. 1967], Recently, it has been proved that when Prozime-10 (P-10), a serine protease extracted from cultured broth of Aspergillus malleus [Ito and Sugiura, 1968;Nagalani, 1966], is effective in various experimental inflammations [Tasaka et al.…”