Inhibition of Dendritic Cell Maturation by Malaria Is Dose Dependent and Does Not RequirePlasmodium falciparumErythrocyte Membrane Protein 1

Abstract

Red blood cells infected with Plasmodium falciparum (iRBCs) have been shown to modulate maturation of human monocyte-derived dendritic cells (DCs), interfering with their ability to activate T cells. Interaction between Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) and CD36 expressed by DCs is the proposed mechanism, but we show here that DC modulation does not require CD36 binding, PfEMP1, or contact between DCs and infected RBCs and depends on the iRBC dose. iRBCs expressing a PfEMP1 variant that binds chondroitin sulfate A (CSA) but not CD36 were phagocytosed, inhibited lipopolysaccharide (LPS)-induced phenotypic maturation and cytokine secretion, and abrogated the ability of DCs to stimulate allogeneic T-cell proliferation. CD36-and CSA-binding iRBCs showed comparable inhibition. P. falciparum lines rendered deficient in PfEMP1 expression by targeted gene knockout or knockdown also inhibited LPS-induced phenotypic maturation, and separation of DCs and iRBCs in transwells showed that inhibition was not contact dependent. Inhibition was observed at an iRBC:DC ratio of 100:1 but not at a ratio of 10:1. High doses of iRBCs were associated with apoptosis of DCs, which was not activation induced. Lower doses of iRBCs stimulated DC maturation sufficient to activate autologous T-cell proliferation. In conclusion, modulation of DC maturation by P. falciparum is dose dependent and does not require interaction between PfEMP1 and CD36. Inhibition and apoptosis of DCs by high-dose iRBCs may or may not be physiological. However, our observation that low-dose iRBCs initiate functional DC maturation warrants reevaluation and further investigation of DC interactions with blood-stage P. falciparum.Dendritic cells (DCs) are specialized antigen-presenting cells that regulate both innate and adaptive immune responses and play a critical role in the initiation of primary T-cell responses. To function effectively as antigen-presenting cells, they undergo a process of maturation, characterized by increased expression of costimulator, major histocompatibility complex and adhesion molecules, and secretion of proinflammatory cytokines (reviewed in reference 35). DC maturation is usually activated by pathogens through ligation of pattern recognition receptors, such as Toll-like receptors, but may also be initiated by inflammatory cytokines and endogenous signals of cellular damage (reviewed in references 29 and 34).It has been suggested that modulation of DC function by the malaria parasite Plasmodium falciparum contributes to both the delayed acquisition of antimalarial immunity as well as immunosuppression associated with acute malaria infection. Urban et al. (50,52) showed that red blood cells infected with P. falciparum (iRBCs) at 100 iRBCs per DC inhibit maturation of human monocyte-derived DCs and interfere with their ability to activate T-cell responses. Interaction between the parasite protein P. falciparum erythrocyte membrane protein 1 (PfEMP1), expressed on the surfaces of iRBCs, and the DC scavenger receptor...

Dendritic Cells in Plasmodium Infection

“…These findings were taken further to show that this process involved the binding of iRBC to CD36 on DCs [24], which is a multiligand scavenger receptor known to bind apoptotic cells, against which immune responses are often not desired. More recent in vitro studies have shown additional suppressive effects not involving parasite binding to CD36 but implicating chondroitin sulphate-A, and also noncontact mechanisms [25]. Furthermore, this study showed that high doses of parasites (100 iRBC:1 DC) were suppressive and caused DC apoptosis, whilst low doses (10:1) activated DCs.…”

“…The capacity of murine splenic CD11c + DCs to become activated and to present antigen to T cells is believed to change over the course of infection [25,40,42], and this observation may reconcile some of the heterogenous findings that showed either activation or suppression of DC function associated with malaria. In simple terms, early on in infection when parasite density is relatively low, IL-12-mediated mechanisms induce IFN-γ-producing CD4 + T cells.…”

Evaluating antidisease immunity to malaria and implications for vaccine design

“…Conversely, lipopolysaccharide (LPS)‐stimulated dendritic cells (DCs) responded appropriately 110. This immunomodulation of DCs by iRBCs was initially thought to depend on P. falciparum erythrocyte membrane protein (PfEMP)‐1; 110,111 however, ‘stunning’ of DCs was later shown to be PfEMP‐1‐independent 112. Subsequent reports showed a role of hemozoin in the functional impairment of DCs and monocyte/macrophages 108.…”

From Secretome Analysis to Immunology

“…fungi and some protozoans) do not and have to face the immune system during their lifetime in the host organism. Perturbing DC differentiation and/or maturation is a strategy that is obviously followed by some slowly dividing pathogens as shown by the examples of Mycobacterium leprae (34) and Plasmodium falciparum (35). Although the mechanism used by M. leprae is still not clear, recent work has shown that red blood cells infected by P. falciparum are able to prevent both DC activation and the triggering of a Th1 response (35).…”

“…Perturbing DC differentiation and/or maturation is a strategy that is obviously followed by some slowly dividing pathogens as shown by the examples of Mycobacterium leprae (34) and Plasmodium falciparum (35). Although the mechanism used by M. leprae is still not clear, recent work has shown that red blood cells infected by P. falciparum are able to prevent both DC activation and the triggering of a Th1 response (35). It has been shown that this effect can be mediated by the binding of an endogenous glycan, chondroitin sulfate A, to the surface of infected red blood cells (35).…”