Inhibition of cytokine and surface antigen expression in LPS-stimulated murine macrophages by triethylene glycol dimethacrylate

Eckhardt, Alexander; Harorli, Tolga; Limtanyakul, Jirakant; Hiller, Karl‐Anton; Bosl, C.; Bolay, Carola; Reichl, Franz‐Xaver; Schmalz, Gottfried; Schweikl, Helmut

doi:10.1016/j.biomaterials.2008.09.024

Cited by 53 publications

(54 citation statements)

References 48 publications

(76 reference statements)

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“…The pattern was similar for exposure periods from 6 hrs to 48 hrs, but generally the amount of cytokines was greatest at 24-hour exposure (Eckhardt et al, 2009a). Under these experimental conditions, down-regulation responded to even small changes in TEGDMA concentrations that had not been observed in other cellular pathways (Spagnuolo et al, 2004;Samuelsen et al, 2007).…”

Section: Inflammation Markersmentioning

confidence: 60%

“…In contrast, CD54 (= ICAM1) was up-regulated after 24 and 48 hrs of exposure, and MHC-II antigens were also up-regulated at 24 hrs of exposure to up to 0.5 mM TEGDMA, then down-regulated, and at 6 and 48 hrs of exposure time, no significant change was observed at all (Eckhardt et al, 2009a).…”

Section: Inflammation Markersmentioning

confidence: 80%

“…For MHC-II, almost no effect was detected (Eckhardt et al, 2009a). These cell-surface antigens are involved in LPS/TLR4 recognition and the following signal transduction processes.…”

Section: Inflammation Markersmentioning

confidence: 98%

“…The LPS-induced intracellular signaling cascade through the MyD88-dependent pathway generates ROS. Activation of further signaling may depend on the correct ROS concentration, which eventually was impaired by the TEGDMA-induced ROS increase (Eckhardt et al, 2009a). aPoPtoSiS TEGDMA and HEMA have been shown to disturb cellular homeostasis, finally leading to apoptosis .…”

Section: Inflammation Markersmentioning

confidence: 99%

“…TEGDMA had generally minor effects on the expression of cell-surface antigens. Some stimulation was observed, e.g., with MHC-I, CD 86, but only 2 mM TEG-DMA significantly increased CD 54 (ICAM 1) over the level induced by 25 µg/mL LPS (Eckhardt et al, 2009a).…”

Section: Inflammation Markersmentioning

confidence: 99%

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Toll-like Receptors, LPS, and Dental Monomers

2011

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Unreacted monomers released from dental resin-based composites at non-cytotoxic concentrations cause a depletion of glutathione and an increase of reactive oxygen species (ROS), leading to, e.g., DNA damage and apoptosis. ROS-sensitive MAP-kinases are activated by HEMA and TEGDMA. MAP-kinases are also involved in the bacteria-triggered cell responses of the innate immune system, e.g., after bacterial lipopolysaccharide (LPS) binding to the Toll-like receptor (TLR) 4. Therefore, both bacteria and monomers imply environmental stress to pulp tissue, and they may influence the target cell reactions in a combined way. In macrophages, cell-surface antigens and cytokines were up-regulated after exposure to LPS, but TEGDMA caused a significant down-regulation. Regulation was dependent on exposure time, indicating that LPS and TEGDMA act differently on MAP-kinases. Furthermore, the cell type played a decisive role. Inhibition of the immune response may result in a decrease in inflammatory symptoms and/or a reduced defense capacity against bacteria.

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Section: Inflammation Markersmentioning

confidence: 60%

Section: Inflammation Markersmentioning

confidence: 80%

“…For MHC-II, almost no effect was detected (Eckhardt et al, 2009a). These cell-surface antigens are involved in LPS/TLR4 recognition and the following signal transduction processes.…”

Section: Inflammation Markersmentioning

confidence: 98%

Section: Inflammation Markersmentioning

confidence: 99%

Section: Inflammation Markersmentioning

confidence: 99%

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Toll-like Receptors, LPS, and Dental Monomers

2011

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Macrophage and dendritic cell phenotypic diversity in the context of biomaterials

Kou

Babensee

2010

J Biomedical Materials Res

168

173

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Macrophages (Mϕ) and dendritic cells (DCs) are critical antigen presenting cells that play pivotal roles in host responses to biomaterial implants. Although Mϕs have been widely studied for their roles in the inflammatory responses against biomaterials, the roles that DCs play in the host responses toward implanted materials have only recently been explored. DCs are of significant research interest because of the emergence of a large number of combination products that cross-traditional medical device boundaries. These products combine biomaterials with biologics, including cells, nucleic acids, and/or proteins. The biomaterial component may evoke an inflammatory response, primarily mediated by neutrophils and Mϕs, whereas the biologic component may elicit an immunogenic immune response, initiated by DCs involving lymphocyte activation. Control of Mϕ phenotypic balance from proinflammatory M1 to reparative M2 is a goal of investigators to optimize the host response to biomaterials. Similarly, control of DC phenotype from proinflammatory to toleragenic is of interest in vaccine delivery and tissue engineering/transplantation situations, respectively. This review discusses the interconnection between innate and adaptive immunity, the comparative and contrasting phenotypes and roles of Mϕs and DCs in immunity, their responses to biomaterials and the strategies to modulate their phenotype for applications in tissue engineering and vaccine delivery. Furthermore, the collaboration between and unique roles of DCs and Mϕs needs to be addressed in future studies to gain a more complete picture of host responses toward combination products.

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Increasing the repeating units of ethylene glycol‐based dimethacrylates directed toward reduced oxidative stress and co‐stimulatory factors expression in human monocytic cells

Tamura

Fukumoto

Yui

et al. 2014

J Biomedical Materials Res

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The ethylene glycol-based dimethacrylates are commonly used in biomaterials and dental restorative materials as a cross-linking agent. In this study, toxic effect of triethylene glycol dimethacrylate (TEGDMA) and poly(ethylene glycol) dimethacrylates (PEG-DMAs) with various ethylene glycol repeating units was investigated in terms of cytotoxicity, oxidative stress, and the expression of co-stimulatory factors in human leukemia cell line (THP-1 cells) to verify the effect of ethylene glycol repeating units. Note that the 1-octanol/water partition coefficient of PEG-based dimethacrylates decreased with increasing the ethylene glycol repeating units, indicating that the hydrophilicity of PEG-DMAs increased with ethylene glycol repeating units. The toxic effect of PEG-DMAs such as cytotoxicity, oxidative stress, and the expression of CD86 in treated THP-1 cells are reduced with increasing the ethylene glycol repeating units in PEG-DMAs. However, the expression of CD54 in treated THP-1 cells was not influenced with the ethylene glycol repeating units and the maximal expression level of CD54 was observed at the concentration range of 2-4 mM for all samples. Accordingly, hydrophilic character of PEG-DMAs with long ethylene glycol chains definitely alleviates the some toxic aspect of PEG-based DMAs. This finding would provide important insight into the design of new biomaterials and dental materials with superior biocompatibility.

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Inhibition of cytokine and surface antigen expression in LPS-stimulated murine macrophages by triethylene glycol dimethacrylate

Cited by 53 publications

References 48 publications

Toll-like Receptors, LPS, and Dental Monomers

Toll-like Receptors, LPS, and Dental Monomers

Macrophage and dendritic cell phenotypic diversity in the context of biomaterials

Increasing the repeating units of ethylene glycol‐based dimethacrylates directed toward reduced oxidative stress and co‐stimulatory factors expression in human monocytic cells

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