Inhibition of Cyclopropane Fatty Acid Synthesis in the Membrane of Halophilic Halomonas socia CKY01 by Kanamycin

Lee, Hye Soo; Lee, Hong-Ju; Kim, Byungchan; Kim, Su-Hyeon; Cho, Do-Hyun; Jung, Hee-Joo; Bhatia, Shashi Kant; Choi, Kwon‐Young; Kim, Wooseong; Lee, Jongbok; Lee, Sang Ho; Yang, Yung‐Hun

doi:10.1007/s12257-022-0086-9

Cited by 6 publications

(1 citation statement)

References 41 publications

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“…Cells were pre-cultured in lysogeny broth (LB) containing 10 g/l tryptone, 5 g/l yeast extract, and 5 g/l sodium chloride at 37°C [ 27 ]. Pre-culture was performed by inoculating a single colony of E. coli BL21 (DE3) picked from LB agar plates into 5 ml of LB medium.…”

Section: Methodsmentioning

confidence: 99%

Tryptophan-Based Hyperproduction of Bioindigo by Combinatorial Overexpression of Two Different Tryptophan Transporters

Kim,

Ham,

Shin

et al. 2023

J. Microbiol. Biotechnol.

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Indigo is a valuable, natural blue dye that has been used for centuries in the textile industry. The large-scale commercial production of indigo relies on its extraction from plants and chemical synthesis. Studies are being conducted to develop methods for environment-friendly and sustainable production of indigo using genetically engineered microbes. Here, to enhance the yield of bioindigo from an E. coli whole-cell system containing tryptophanase (TnaA) and flavin-containing monooxygenase (FMO), we evaluated tryptophan transporters to improve the transport of aromatic compounds, such as indole and tryptophan, which are not easily soluble and passable through cell walls. Among the three transporters, Mtr, AroP, and TnaB, AroP enhanced indigo production the most. The combination of each transporter with AroP was also evaluated, and the combination of AroP and TnaB showed the best performance compared to the single transporters and two transporters. Bioindigo production was then optimized by examining the culture medium, temperature, isopropyl β-D-1-thiogalactopyranoside concentration, shaking speed (rpm), and pH. The novel strain containing aroP and tnaB plasmid with tnaA and FMO produced 8.77 mM (2.3 g/l) of bioindigo after 66 h of culture. The produced bioindigo was further recovered using a simple method and used as a watercolor dye, showing good mixing with other colors and color retention for a relatively long time. This study presents an effective strategy for enhancing indigo production using a combination of transporters.

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Section: Methodsmentioning

confidence: 99%