Inhibition of cuticle deposition and chitin biosynthesis by a new insect growth regulator, buprofezin, in Nilaparvata lugens Stal.

Uchida, Matazaemon; Asai, Toshiro; Sugimoto, Tatsuyoshi

doi:10.1271/bbb1961.49.1233

Cited by 59 publications

(29 citation statements)

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“…S1) highly selective to sucking agricultural pests that have also been proposed to interfere with chitin biosynthesis or cuticle formation (16,17). Etoxazole is an oxazoline acaricide widely used against pest mite species but with limited activity on insects (18).…”

mentioning

confidence: 99%

Resistance mutation conserved between insects and mites unravels the benzoylurea insecticide mode of action on chitin biosynthesis

Douris

Steinbach

Panteleri

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

159

166

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Despite the major role of chitin biosynthesis inhibitors such as benzoylureas (BPUs) in the control of pests in agricultural and public health for almost four decades, their molecular mode of action (MoA) has in most cases remained elusive. BPUs interfere with chitin biosynthesis and were thought to interact with sulfonylurea receptors that mediate chitin vesicle transport. Here, we uncover a mutation (I1042M) in the chitin synthase 1 (CHS1) gene of BPU-resistant Plutella xylostella at the same position as the I1017F mutation reported in spider mites that confers etoxazole resistance. Using a genome-editing CRISPR/Cas9 approach coupled with homology-directed repair (HDR) in Drosophila melanogaster, we introduced both substitutions (I1056M/F) in the corresponding fly CHS1 gene (kkv). Homozygous lines bearing either of these mutations were highly resistant to etoxazole and all tested BPUs, as well as buprofezin-an important hemipteran chitin biosynthesis inhibitor. This provides compelling evidence that BPUs, etoxazole, and buprofezin share in fact the same molecular MoA and directly interact with CHS. This finding has immediate effects on resistance management strategies of major agricultural pests but also on mosquito vectors of serious human diseases such as Dengue and Zika, as diflubenzuron, the standard BPU, is one of the few effective larvicides in use. The study elaborates on how genome editing can directly, rapidly, and convincingly elucidate the MoA of bioactive molecules, especially when target sites are complex and hard to reconstitute in vitro

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mentioning

confidence: 99%

Resistance mutation conserved between insects and mites unravels the benzoylurea insecticide mode of action on chitin biosynthesis

Douris

Steinbach

Panteleri

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

159

166

View full text Add to dashboard Cite

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“…Its mode of action resembles that of benzoylphenyl ureas, although its structure is not analogous. The compound inhibits incorporation of 3H-glucose and N-acetyl-D-3 H-glucosamine into chitin (Izawa et al 1985;Uchida et al 1985). The characteristic symptoms in the greenhouse whitefly Trialeurodes vaporariorum resembled those obtained with benzoylphenyl ureas (De Cock and Degheele 1991).…”

Section: Chitin Synthesis Inhibitionmentioning

confidence: 88%

Biochemical Processes Related to Insecticide Action: an Overview

Ishaaya

2001

Biochemical Sites of Insecticide Action and Resistance

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“…Studies with Nilaparvata lugens Stål (Hemiptera: Delphacidae) showed a deregulation pattern in the deposition of cuticle chitin caused by application of buprofezin (UCHIDA et al, 1985).…”

Section: Bioassay I -Fecundity and Fertility Of Dichelops Melacanthusmentioning

confidence: 99%

Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes

Cremonez

Pinheiro

Falleiros

et al. 2017

SCA

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The use of insecticides known as insect growth regulators, which are considered more selective to natural enemies, may be an alternative to integrated pest management of stink bugs of the main crops in Brazil. The objective of this study was to evaluate histological changes in the reproductive system of Dichelops melacanthus as well as female fecundity and egg fertility after use of growth-regulating insecticides. The insecticides used were buprofezin (a chitin biosynthesis inhibitor) at a sublethal concentration (LC 30 of 2.99 g L -1 ) and pyriproxyfen (a juvenile hormone analog) at a sublethal concentration (LC 30 of 8.35 mL L -1 ). A completely randomized experimental design was used, with 10 repetitions and 10 insects per experimental unit. Two bioassays were performed: in the first bioassay, fecundity (eggs/ female) and fertility (nymph eclosion) of the insects that survived the insecticides were evaluated; in the second bioassay, morphological alterations of the ovarioles of adult females and of the testes of adult male insects that survived the insecticides were evaluated. Buprofezin and pyriproxyfen did not affect the adult sex ratio or female fecundity. Pyriproxyfen reduced the percentage of ecloded nymphs (71.6%) compared with that of the control and buprofezin (96.4 and 90.6%, respectively) treatments and had an ovicidal effect, with direct and indirect action on embryogenesis. Morphological changes were observed in both treatments with buprofezin and pyriproxyfen. The alterations observed in female and male reproductive systems may occur by the action of buprofezin and pyriproxyfen on the morphology of both ovarioles and testes. Key words: Dose-response curve. Endocrine disruption. Green-belly stink bug. Insect growth regulators. Reproductive organ morphology. ResumoA utilização de inseticidas conhecidos como reguladores de crescimento dos insetos, considerados mais seletivos a inimigos naturais, pode ser uma alternativa no manejo integrado no que se refere ao controle de percevejos-pragas das principais culturas no Brasil. Os objetivos deste trabalho foram avaliar alterações histológicas causadas no aparelho reprodutor de Dichelops melacanthus e a fecundidade das fêmeas e fertilidade de ovos, após o uso de inseticidas reguladores de crescimento. utilizados foram buprofezina (inibidor da biossíntese de quitina), na concentração subletal CL 30 2,99 g L -1 , e o piriproxifem (análogo do hormônio juvenil) na concentração subletal CL 30 8,35 mL L -1 . O delineamento experimental foi inteiramente casualizado, com 10 repetições e 10 insetos por repetição. Foram realizados dois bioensaios: no primeiro foi avaliada a fecundidade (número de ovos/fêmea) e a fertilidade (número de ninfas eclodidas) dos insetos que sobreviveram aos produtos. No segundo bioensaio, foram avaliadas as alterações morfológicas de ovaríolos de fêmeas e testículo de machos de percevejos adultos, que sobreviveram à ação dos inseticidas. Buprofezina e piriproxifem não afetaram a razão sexual de adultos ou mesmo a fecundidade das ...

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Inhibition of cuticle deposition and chitin biosynthesis by a new insect growth regulator, buprofezin, in Nilaparvata lugens Stal.

Cited by 59 publications

References 0 publications

Resistance mutation conserved between insects and mites unravels the benzoylurea insecticide mode of action on chitin biosynthesis

Resistance mutation conserved between insects and mites unravels the benzoylurea insecticide mode of action on chitin biosynthesis

Biochemical Processes Related to Insecticide Action: an Overview

Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes

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