Inhibition of creatine kinase activity from rat cerebral cortex by 3-hydroxykynurenine

Cornelio, Andrea Renata; Rodrigues-Junior, Valnês S.; Rech, Virgínia Cielo; Wyse, Ângela T. S.; Dutra-Filho, Carlos Severo; Wajner, Moaçir; Wannmacher, Clóvis Milton Duval

doi:10.1016/j.brainres.2006.09.087

Cited by 5 publications

(1 citation statement)

References 54 publications

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“…After pre-incubation (10 min at 37°C), the slices were incubated for 2 h at 37°C in the presence of DL-3-HK (100 μM) on a shaking water bath. Ascorbic acid (final concentration: 500 μM) was added to the wells to minimize the effects of spontaneous oxidation of DL-3-HK (Cornelio et al, 2006). …”

Section: Methodsmentioning

confidence: 99%

Xanthurenic Acid Formation from 3-Hydroxykynurenine in the Mammalian Brain: Neurochemical Characterization and Physiological Effects

Sathyasaikumar

Tararina

et al. 2017

Neuroscience

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Xanthurenic acid (XA), formed from 3-hydroxykynurenine (3-HK) in the kynurenine pathway of tryptophan degradation, may modulate glutamatergic neurotransmission by inhibiting the vesicular glutamate transporter and/or activating Group II metabotropic glutamate receptors. Here we examined the molecular and cellular mechanisms by which 3-HK controls the neosynthesis of XA in rat, mouse and human brain, and compared the physiological actions of 3-HK and XA in the rat brain. In tissue homogenates, XA formation from 3-HK was observed in all three species and traced to a major role of kynurenine aminotransferase II (KAT II). Transamination of 3-HK to XA was also demonstrated using human recombinant KAT II. Neosynthesis of XA was significantly increased in the quinolinate-lesioned rat striatum, indicating a non-neuronal localization of the process. Studies using rat cortical slices revealed that newly produced XA is rapidly released into the extracellular compartment, and that XA biosynthesis can be manipulated experimentally in the same way as the production of kynurenic acid from kynurenine (omission of Na+ or glucose, depolarizing conditions, or addition of 2-oxoacids). The synthesis of XA from 3-HK was confirmed in vivo by striatal microdialysis. In slices from the rat hippocampus, both 3-HK and XA reduced the slopes of dentate gyrus field EPSPs. The effect of 3-HK was reduced in the presence of the KAT inhibitor aminooxyacetic acid. Finally, both 3-HK and XA reduced the power of gamma-oscillatory activity recorded from the hippocampal CA3 region. Endogenous XA, newly formed from 3-HK, may therefore play a physiological role in attentional and cognitive processes.

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Section: Methodsmentioning

confidence: 99%

Xanthurenic Acid Formation from 3-Hydroxykynurenine in the Mammalian Brain: Neurochemical Characterization and Physiological Effects

Sathyasaikumar

Tararina

et al. 2017

Neuroscience

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Creatine and Creatine Kinase in Health and Disease – A Bright Future Ahead?

Wyss¹,

Braissant

Pischel³

et al.

Creatine and Creatine Kinase in Health and Disease

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Identification of l-3-hydroxykynurenine O-sulfate in the buccal gland secretion of the parasitic lamprey, Lethenteron japonicum

et al. 2012

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Parasitic lampreys are known to secrete proteins having anticoagulant and vasodilator activities from the buccal glands during feeding on their host's blood. However, small molecules in the secretion have never been explored in detail. We examined the secretion of Japanese liver lamprey (Lethenteron japonicum) for small molecules and found an intensely fluorescent substance upon gel filtration. After purification by anion-exchange chromatography and reversed-phase HPLC, structure of the compound was determined to be L-3-hydroxykynurenine O-sulfate by NMR- and UV-spectrometry, complemented with enzymatic and chemical degradation. In vertebrates, the sulfate ester of 3-hydroxykynurenine is a compound that has been regarded as a urinary metabolite of tryptophan but not reported from normal tissues to date. Although the function of this molecule in the buccal glands remains to be elucidated, it is remarkable that the same substance was described in 1960s from two species of blood-sucking insects, Rhodnius prolixus and Triatoma infestans, suggesting its potential role in blood-feeding.

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Inhibition of creatine kinase activity from rat cerebral cortex by 3-hydroxykynurenine

Cited by 5 publications

References 54 publications

Xanthurenic Acid Formation from 3-Hydroxykynurenine in the Mammalian Brain: Neurochemical Characterization and Physiological Effects

Xanthurenic Acid Formation from 3-Hydroxykynurenine in the Mammalian Brain: Neurochemical Characterization and Physiological Effects

Creatine and Creatine Kinase in Health and Disease – A Bright Future Ahead?

Identification of l-3-hydroxykynurenine O-sulfate in the buccal gland secretion of the parasitic lamprey, Lethenteron japonicum

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