Inhibition of cortical neural networks using infrared laser

Xia, Qingling; Nyberg, Tobias

doi:10.1002/jbio.201800403

Cited by 24 publications

(36 citation statements)

References 35 publications

(52 reference statements)

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“…Calcium imaging experiments in the cortex indicate that INS could affect intracellular calcium dynamics in both neurons and astrocytes (43). In addition to INS-induced excitation, inhibitory effects on spiking activity (44) and AP conduction (45) would also occur when appropriate laser parameters (such as laser operation mode, pulse width, and frequency) are selected, possibly resulting from the activation of voltage-gated K + channels (46).…”

Section: Discussionmentioning

confidence: 99%

Nonthermal and reversible control of neuronal signaling and behavior by midinfrared stimulation

Liu

Qiao

Chai

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

145

107

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Various neuromodulation approaches have been employed to alter neuronal spiking activity and thus regulate brain functions and alleviate neurological disorders. Infrared neural stimulation (INS) could be a potential approach for neuromodulation because it requires no tissue contact and possesses a high spatial resolution. However, the risk of overheating and an unclear mechanism hamper its application. Here we show that midinfrared stimulation (MIRS) with a specific wavelength exerts nonthermal, long-distance, and reversible modulatory effects on ion channel activity, neuronal signaling, and sensorimotor behavior. Patch-clamp recording from mouse neocortical pyramidal cells revealed that MIRS readily provides gain control over spiking activities, inhibiting spiking responses to weak inputs but enhancing those to strong inputs. MIRS also shortens action potential (AP) waveforms by accelerating its repolarization, through an increase in voltage-gated K+ (but not Na+) currents. Molecular dynamics simulations further revealed that MIRS-induced resonance vibration of –C=O bonds at the K+ channel ion selectivity filter contributes to the K+ current increase. Importantly, these effects are readily reversible and independent of temperature increase. At the behavioral level in larval zebrafish, MIRS modulates startle responses by sharply increasing the slope of the sensorimotor input–output curve. Therefore, MIRS represents a promising neuromodulation approach suitable for clinical application.

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Section: Discussionmentioning

confidence: 99%

Nonthermal and reversible control of neuronal signaling and behavior by midinfrared stimulation

Liu

Qiao

Chai

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

145

107

View full text Add to dashboard Cite

show abstract

“…Generally, a Gaussian beam profile is used to determine the threshold of monospectral laser in an in vitro setting. [68][69][70] However, in the current study, a square gradient beam profile was chosen as appropriate for a dual laser in order to simplify the experimental procedures. We used a square-core fiber to create a square beam profile because the use of the fiber is easier and more affordable than the phase gating 71 or a micromirror device.…”

Section: Discussionmentioning

confidence: 99%

High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells

et al. 2020

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Significance: Photobiomodulation is a well-established therapeutic modality. However, the mechanism of action is poorly understood, due to lack of research in the causal relationship between the near-infrared (NIR) light irradiation and its specific biological effects, hindering broader applications of this technology. Aim: Since biological chromophores typically show several absorption peaks, we determined whether specific effects of photobiomodulation are induced with a combination of two wavelengths at a certain range of irradiance only, rather than a single wavelength of NIR light. Approach: In order to analyze a wide array of combinations of multispectral NIR light at various irradiances efficiently, we developed a new optical platform equipped with two distinct wavelengths of NIR lasers by high-throughput multiple dosing for single-cell live imaging. Two wavelengths of 1064 and 1270 nm were selected based on their photobiomodulatory effects reported in the literature. Results: A specific combination of wavelengths at low irradiances (250 to 400 mW∕cm 2 for 1064 nm and 55 to 65 mW∕cm 2 for 1270 nm) modulates mitochondrial retrograde signaling, including intracellular calcium and reactive oxygen species in T cells. The time-dependent density functional theory computation of binding of nitric oxide (NO) to cytochrome c oxidase indicates that the illumination with NIR light could result in the NO release, which might be involved in these changes. Conclusions: This optical platform is a powerful tool to study causal relationship between a specific parameter of NIR light and its biological effects. Such a platform is useful for a further mechanistic study on not only photobiomodulation but also other modalities in photomedicine.

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“…In addition to IR neural stimulation, suppression of neural activities and blocking of action potentials by pulsed IR light have also been reported in both in vivo and in vitro studies [21][22][23][24][25][26][27][28][29][30][31]. IR neural inhibition effects were observed during several IR nerve stimulation studies in both the central [22,24] and the peripheral [23,31] nerve system.…”

Section: Introductionmentioning

confidence: 89%

Infrared inhibition and waveform modulation of action potentials in the crayfish motor axon

Zhu¹,

Lin²,

Sander³

2019

Biomed. Opt. Express

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The infrared (IR) inhibition of axonal activities in the crayfish neuromuscular preparation is studied using 2 µm IR light pulses with varying durations. The intracellular neuronal activities are monitored with two-electrode current clamp, while the IR-induced temperature changes are measured by the open patch technique simultaneously. It is demonstrated that the IR pulses can reversibly shape or block locally initiated action potentials. Suppression of the AP amplitude and duration and decrease in axonal excitability by IR pulses are quantitatively analyzed. While the AP amplitude and duration decrease similarly during IR illumination, it is discovered that the recovery of the AP duration after the IR pulses is slower than that of the AP amplitude. An IR-induced decrease in the input resistance (8.8%) is detected and discussed together with the temperature dependent changes in channel kinetics as contributing factors for the inhibition reported here.

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Inhibition of cortical neural networks using infrared laser

Cited by 24 publications

References 35 publications

Nonthermal and reversible control of neuronal signaling and behavior by midinfrared stimulation

Nonthermal and reversible control of neuronal signaling and behavior by midinfrared stimulation

High-throughput single-cell live imaging of photobiomodulation with multispectral near-infrared lasers in cultured T cells

Infrared inhibition and waveform modulation of action potentials in the crayfish motor axon

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