Inhibition of contractility during the early phase of total ischaemia in the working heart. Recovery during reperfusion

Steinmann, C.M.L.; Lochner, Amanda; Niesler, R. A.

doi:10.1007/bf01906862

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…Contracture development during ischaemia might already be evident after about 9 min in a perfused rat heart (Steinmann et al 1987). Reperfusion of a contractured heart leads to poor mechanical function and low coronary flow (Humphrey et al 1981), at least during the early stages of ischaemia.…”

Section: M L Steinmann and M Storm [ The Conservation Of Mitochondrmentioning

confidence: 99%

The conservation of mitochondrial function by ischaemia – the ischaemia paradox

Steinmann

Storm

1997

Acta Physiologica Scandinavica

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The aim of this study was to compare oxidative phosphorylation of rat heart mitochondria, isolated: (i) immediately after excision of the heart; (ii) after 60 min of low flow normoxic perfusion; (iii) after 60 min of high flow normoxic perfusion;: (iv) after 30 min of no-flow ischaemia and (v) after 30 min ischaemia followed by 20 min reperfusion. The results, using a retrogradely perfused rat hart as a model, show that mitochondrial oxidative phosphorylation after 30 min of no-flow ischaemia showed no difference from oxidative phosphorylation measured on mitochondria isolated directly after excision of the heart. However, after 30 min no-flow ischaemia followed by 20 min of reperfusion, oxidative phosphorylation deteriorated in comparison with oxidative phosphorylation after 30 min of ischaemia only. Furthermore, oxidative phosphorylation after 60 min of non-ischaemic high flow perfusion (thus high coronary flow), compared better with oxidative phosphorylation after ischaemia-reperfusion than with oxidative phosphorylation after ischaemia alone.

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Section: M L Steinmann and M Storm [ The Conservation Of Mitochondrmentioning

confidence: 99%

The conservation of mitochondrial function by ischaemia – the ischaemia paradox

Steinmann

Storm

1997

Acta Physiologica Scandinavica

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View full text Add to dashboard Cite

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Predicting functional recovery from ischemia in the rat myocardium

1992

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Depletion of high-energy phosphates, accumulation of inorganic phosphate and intracellular acidosis have each been proposed as important events in the transition from reversible to irreversible ischemic injury. To assess whether each variable is predictive of functional recovery on reperfusion, these were measured in the isolated isovolumic rat heart using 31P NMR. Perfused hearts were subjected to either 10, 12 or 40 min of normothermic ischemia followed by 40 min of reperfusion. Hearts were then freeze-clamped for further analysis of phosphate metabolites by NMR and ion chromatography. High-energy phosphates, Pi, phosphomonoesters and pH were measured by 31P NMR spectroscopy at 2 minute intervals. Heart rate and developed pressure were monitored simultaneously. All hearts undergoing 10 min of ischemia and 40% of hearts subjected to 12 min of ischemia demonstrated good functional recovery. The remainder of hearts ischemic for 12 min went into contracture on reperfusion with little return of function. Hearts subject to 40 min of ischemia went into ischemic contracture and showed no recovery on reperfusion. Intracellular pH, [ATP], and [Pi] measured prior to reperfusion did not predict the extent of recovery. However, phosphomonoesters were detected prior to reperfusion in all hearts that did not recover well, but were not observed in hearts that showed good mechanical recovery. Analysis of tissue extracts by 31P NMR and ion chromatography indicated that the most prominent components of the phosphomonoesters were glucose 6-phosphate, alpha-glycerol phosphate and AMP. In conclusion, of the various phosphorus metabolites that can be measured by 31P NMR, only one group, the phosphomonoesters, was predictive of functional recovery.

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Inhibition of contractility during the early phase of total ischaemia in the working heart. Recovery during reperfusion

Cited by 2 publications

References 14 publications

The conservation of mitochondrial function by ischaemia – the ischaemia paradox

The conservation of mitochondrial function by ischaemia – the ischaemia paradox

Predicting functional recovery from ischemia in the rat myocardium

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