Inhibition of condensation of human Y chromosome by the fluorochrome Hoechst 33258 in a mouse-human cell hybrid

Marcus, Menashe; Nattenberg, Alona; Goitein, Ruth; Nielsén, Karin; Gropp, A.

doi:10.1007/bf00291921

Cited by 11 publications

(3 citation statements)

References 13 publications

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“…However, targeting a fluorescent protein to the nucleus, for example using BacMam technology (UNIT 12.30), allows the imaging of the nucleus in both live and fixed cells. Unlike nucleic acid stains, which may have effects on nuclear function (Marcus et al, 1979;Rocchi et al, 1979;Fried et al, 1982), transient expression of a nuclear targeted fluorescent protein lasts 3 to 5 days and has no observable side effects on nuclear function. Commercially available constructs include premade subcellular vectors from Evrogen and Clontech and Evrogen or BacMam reagents against nuclear targets, including CellLight Nucleus-CFP, -GFP, or -RFP reagents and CellLight Histone 2B-GFP and -RFP reagents (Life Technologies).…”

Section: A Discussion Of Dyes For Nucleic Acids (Also See Recipe For mentioning

confidence: 99%

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non‐Vesicular Organelles

2013

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A wide range of fluorescent dyes and reagents exist for labeling organelles in live and fixed cells. Choosing between them can sometimes be confusing, and optimization for many of them can be challenging. Presented here is a discussion on the commercially‐available reagents that have shown the most promise for each organelle of interest, including endoplasmic reticulum/nuclear membrane, Golgi apparatus, mitochondria, nucleoli, and nuclei, with an emphasis on localization of these structures for microscopy. Included is a featured reagent for each structure with a recommended protocol, troubleshooting guide, and example image. Curr. Protoc. Cytom. 66:12.31.1‐12.34.24. © 2013 by John Wiley & Sons, Inc.

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Section: A Discussion Of Dyes For Nucleic Acids (Also See Recipe For mentioning

confidence: 99%

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non‐Vesicular Organelles

2013

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“…However, targeting a fluorescent protein to the nucleus, for example using BacMam technology (Dolman et al., 2013 UNIT 12.30) , allows the imaging of the nucleus in both live and fixed cells. Unlike with nucleic acid stains, which may have effects on nuclear function (Fried et al., 1982; Marcus et al., 1979; Rocchi et al., 1979), transient expression of a nuclear‐targeted fluorescent protein lasts 3‐5 days and has no observable side effects on nuclear function. Commercially available constructs include premade subcellular vectors from Evrogen and Clontech or BacMam reagents against nuclear targets, including CellLight™ Nucleus‐CFP, ‐GFP, or ‐RFP reagents and CellLight Histone 2B‐GFP and ‐RFP reagents (Thermo Fisher Scientific).…”

Section: Commentarymentioning

confidence: 99%

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non‐Vesicular Organelles

Kilgore

Dolman

2023

Current Protocols

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A wide range of fluorescent dyes and reagents exist for labeling organelles in live and fixed cells. Choosing between them can lead to confusion, and optimization for many of them can be challenging. Presented here is a discussion on the commercially available reagents that have shown the most promise for each organelle of interest, including endoplasmic reticulum/nuclear membrane, Golgi apparatus, mitochondria, nucleoli, and nuclei, with an emphasis on localization of these structures for microscopy. Included is a featured reagent for each structure with a recommended protocol, troubleshooting guide, and example image.

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“…However, this feature is probably a necessary but not the only prerequisite for delayed condensation. There are other unknown factors relevant to chromosome condensation (Marcus et al, 1979;Rocchi, 1982;Felcher et al, 1982). The present work describes decondensation of Drosophila chromosomes in heterochromatic regions.…”

Section: Introductionmentioning

confidence: 99%

Specific decondensation of heterochromatic regions of Drosophila chromosomes-intrachromosomal and genomic control

et al. 1986

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The genomic and intrachromosomal control of condensation inhibition of heterochromatic regions of mitotic Drosophila chromosomes was studied. It is shown that decondensation is not a local property of the chromosomal regions. In particular the frequency and sometimes the pattern of decondensation is changed with translocations, inversions or loss of chromosomes. In a variety of deletions and inversions of X-chromosomal heterochromatin, which is usually decondensed, the phenomenon is absent or its pattern changed. A material effect is observed in interspecies hybrids between D. melanogaster and D. simulans: paternal chromosomes are neither more condensed or more decondensed under the influence of a maternal genome.

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Inhibition of condensation of human Y chromosome by the fluorochrome Hoechst 33258 in a mouse-human cell hybrid

Cited by 11 publications

References 13 publications

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non‐Vesicular Organelles

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non‐Vesicular Organelles

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non‐Vesicular Organelles

Specific decondensation of heterochromatic regions of Drosophila chromosomes-intrachromosomal and genomic control

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