1979
DOI: 10.1007/bf00291921
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Inhibition of condensation of human Y chromosome by the fluorochrome Hoechst 33258 in a mouse-human cell hybrid

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Cited by 11 publications
(3 citation statements)
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“…However, targeting a fluorescent protein to the nucleus, for example using BacMam technology (UNIT 12.30), allows the imaging of the nucleus in both live and fixed cells. Unlike nucleic acid stains, which may have effects on nuclear function (Marcus et al, 1979;Rocchi et al, 1979;Fried et al, 1982), transient expression of a nuclear targeted fluorescent protein lasts 3 to 5 days and has no observable side effects on nuclear function. Commercially available constructs include premade subcellular vectors from Evrogen and Clontech and Evrogen or BacMam reagents against nuclear targets, including CellLight Nucleus-CFP, -GFP, or -RFP reagents and CellLight Histone 2B-GFP and -RFP reagents (Life Technologies).…”
Section: A Discussion Of Dyes For Nucleic Acids (Also See Recipe For mentioning
confidence: 99%
“…However, targeting a fluorescent protein to the nucleus, for example using BacMam technology (UNIT 12.30), allows the imaging of the nucleus in both live and fixed cells. Unlike nucleic acid stains, which may have effects on nuclear function (Marcus et al, 1979;Rocchi et al, 1979;Fried et al, 1982), transient expression of a nuclear targeted fluorescent protein lasts 3 to 5 days and has no observable side effects on nuclear function. Commercially available constructs include premade subcellular vectors from Evrogen and Clontech and Evrogen or BacMam reagents against nuclear targets, including CellLight Nucleus-CFP, -GFP, or -RFP reagents and CellLight Histone 2B-GFP and -RFP reagents (Life Technologies).…”
Section: A Discussion Of Dyes For Nucleic Acids (Also See Recipe For mentioning
confidence: 99%
“…However, targeting a fluorescent protein to the nucleus, for example using BacMam technology (Dolman et al., 2013 UNIT 12.30) , allows the imaging of the nucleus in both live and fixed cells. Unlike with nucleic acid stains, which may have effects on nuclear function (Fried et al., 1982; Marcus et al., 1979; Rocchi et al., 1979), transient expression of a nuclear‐targeted fluorescent protein lasts 3‐5 days and has no observable side effects on nuclear function. Commercially available constructs include premade subcellular vectors from Evrogen and Clontech or BacMam reagents against nuclear targets, including CellLight™ Nucleus‐CFP, ‐GFP, or ‐RFP reagents and CellLight Histone 2B‐GFP and ‐RFP reagents (Thermo Fisher Scientific).…”
Section: Commentarymentioning
confidence: 99%
“…However, this feature is probably a necessary but not the only prerequisite for delayed condensation. There are other unknown factors relevant to chromosome condensation (Marcus et al, 1979;Rocchi, 1982;Felcher et al, 1982). The present work describes decondensation of Drosophila chromosomes in heterochromatic regions.…”
Section: Introductionmentioning
confidence: 99%