Inhibition of complement-mediated cytotoxicity of antisera by fluid secreted by the seminal vesicle of the house mouse

Peitz, Betsy; Bennett, Dorothea

doi:10.1016/0165-0378(81)90015-2

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…Some seminal proteins bind to sperm or comigrate with sperm through the uterus (Irwin et al 1983; Robinson et al 1987; Carballada and Esponda 1997, 1998) and affect sperm motility (Peitz and Olds‐Clarke 1986; Agrawal and Vanha‐Perttula 1987; Peitz 1988) and capacitation (Huang et al 2000; Kawano and Yoshida 2007). These proteins can also suppress the female immune responses toward sperm (Peitz and Bennett 1981; Anderson and Tarter 1982; Thaler 1989). Disruption at any of these levels could potentially underlie the conspecific fertilization advantage.…”

Section: Discussionmentioning

confidence: 99%

Faster Fertilization Rate in Conspecific Versus Heterospecific Matings in House Mice

Dean

Nachman²

2009

Evolution

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Barriers to gene flow can arise at any stage in the reproductive sequence. Most studies of reproductive isolation focus on premating or postzygotic phenotypes, leaving the importance of differences in fertilization rate overlooked. Two closely related species of house mice, Mus domesticus and M. musculus, form a narrow hybrid zone in Europe, suggesting that one or more isolating factors operate in the face of ongoing gene flow. Here, we test for differences in fertilization rate using laboratory matings as well as in vitro sperm competition assays. In noncompetitive matings, we show that fertilization occurs significantly faster in conspecific versus heterospecific matings and that this difference arises after mating and before zygotes form. To further explore the mechanisms underlying this conspecific advantage, we used competitive in vitro assays to isolate gamete interactions. Surprisingly, we discovered that M. musculus sperm consistently outcompeted M. domesticus sperm regardless of which species donated ova. These results suggest that in vivo fertilization rate is mediated by interactions between sperm, the internal female environment, and/or contributions from male seminal fluid. We discuss the implications of faster conspecific fertilization in terms of reproductive isolation among these two naturally hybridizing species.

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Section: Discussionmentioning

confidence: 99%

Faster Fertilization Rate in Conspecific Versus Heterospecific Matings in House Mice

Dean

Nachman²

2009

Evolution

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“…Seminal fluid has many functions during reproduction, including regulation of sperm motility (Peitz and Olds-Clarke 1986;Agrawal and Vanha-Perttula 1987;Peitz 1988), mediation of sperm capacitation (Huang et al 2000;Kawano and Yoshida 2007), suppression of the female immune system (Peitz and Bennett 1981;Anderson and Tarter 1982;Thaler 1989), and interaction with sperm from other males (Prout and Clark 2000). In mammals, experimental removal of accessory glands results in reduced pregnancy rate, a reduction in the number of young produced, and/or delays in embryonic development (Pang et al 1979;Queen et al 1981;Peitz and Olds-Clarke 1986;O et al 1988;Henault et al 1995;Carballada and Esponda 1999).…”

Section: Introductionmentioning

confidence: 99%

Proteomics and Comparative Genomic Investigations Reveal Heterogeneity in Evolutionary Rate of Male Reproductive Proteins in Mice (Mus domesticus)

Dean

Clark

Findlay

et al. 2009

Molecular Biology and Evolution

130

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Male reproductive fitness is strongly affected by seminal fluid. In addition to interacting with the female environment, seminal fluid mediates important physiological characteristics of sperm, including capacitation and motility. In mammals, the male reproductive tract shows a striking degree of compartmentalization, with at least six distinct tissue types contributing material that is combined with sperm in an ejaculate. Although studies of whole ejaculates have been undertaken in some species, we lack a comprehensive picture of the specific proteins produced by different accessory tissues. Here, we perform proteomic investigations of six regions of the male reproductive tract in mice -- seminal vesicles, anterior prostate, dorsolateral prostate, ventral prostate, bulbourethral gland, and bulbourethral diverticulum. We identify 766 proteins that could be mapped to 506 unique genes and compare them with a high-quality human seminal fluid data set. We find that Gene Ontology functions of seminal proteins are largely conserved between mice and humans. By placing these data in an evolutionary framework, we show that seminal vesicle proteins have experienced a significantly higher rate of nonsynonymous substitution compared with the genome, which could be the result of adaptive evolution. In contrast, proteins from the other five tissues showed significantly lower nonsynonymous substitution, revealing a previously unappreciated level of evolutionary constraint acting on the majority of male reproductive proteins.

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Anticomplementary Activity in Human Semen and Its Possible Importance in Reproduction

Price

Roberts

Green

et al. 1984

American Journal of Reproductive Immunology

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We demonstrated anticomplementary activity on once-washed human sperm, and in normal and vasectomized seminal plasmas. It was demonstrated to be a normal component of human semen. The origin of the activity is proposed to be the seminal plasma with sperm adsorption of activity. The properties of the seminal anticomplementary factor were characterized further, and the molecular size was shown to be less than 3500 daltons. Reduced anticomplementary activity was found to be associated significantly with abnormal semen profiles and infertility in males. The activity in seminal plasma was shown to have no effect on complement-dependent sperm immobilizing antibodies in the serum of an infertile woman, implicating an effect on the post-C3 components of the complement pathway. The inhibition of complement-dependent haemolysis and the lack of inhibition of complement-dependent sperm immobilization by the anticomplementary factor are considered in the implications of the role of seminal anticomplementary activity in reproduction.

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Inhibition of complement-mediated cytotoxicity of antisera by fluid secreted by the seminal vesicle of the house mouse

Cited by 5 publications

References 15 publications

Faster Fertilization Rate in Conspecific Versus Heterospecific Matings in House Mice

Faster Fertilization Rate in Conspecific Versus Heterospecific Matings in House Mice

Proteomics and Comparative Genomic Investigations Reveal Heterogeneity in Evolutionary Rate of Male Reproductive Proteins in Mice (Mus domesticus)

Anticomplementary Activity in Human Semen and Its Possible Importance in Reproduction

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