Inhibition of Circulating miR-194-5p Reverses Osteoporosis through Wnt5a/β-Catenin-Dependent Induction of Osteogenic Differentiation

Mi, Bobin; Yan, Chenchen; Xue, Hang; Chen, Lang; Hu, Liangcong; Hu, Yiqiang; Cao, Faqi; Sun, Yun; Zhou, Wu; Xiong, Yuan; Liu, Guohui

doi:10.1016/j.omtn.2020.07.023

Cited by 21 publications

(14 citation statements)

References 25 publications

(25 reference statements)

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“…Furthermore, Mi et al [ 62 ] reported that miR-194-5p is upregulated in the plasma samples from osteoporosis patients, which is inversely associated with the degree of bone formation in these affected individuals. Subsequently, it was detected that this miRNA inhibits osteoblast differentiation and diminishes bone mass in vivo and impedes osteogenic differentiation in vitro.…”

Section: Osteoporosis and Mirnasmentioning

confidence: 99%

“…Besides, it was found that the target of miR-194-5p is the Wnt-5a protein (WNT5A), whose main function is related to the induction of osteogenic differentiation through the Wnt5a/β-catenin signaling pathway. Accordingly, the increased expression of miR-194-5p during osteoporosis affects osteogenic differentiation and aggravates bone loss via targeting Wnt5a [ 62 ]. In addition, it has been stated that frizzled-4 (FZD4), a protein associated with osteogenic differentiation, is a potential target of miR-1286.…”

Section: Osteoporosis and Mirnasmentioning

confidence: 99%

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The Emerging Role of MicroRNAs in Bone Diseases and Their Therapeutic Potential

et al. 2021

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MicroRNAs (miRNAs) are a class of small (20–24 nucleotides), highly conserved, non-coding RNA molecules whose main function is the post-transcriptional regulation of gene expression through sequence-specific manners, such as mRNA degradation or translational repression. Since these key regulatory molecules are implicated in several biological processes, their altered expression affects the preservation of cellular homeostasis and leads to the development of a wide range of pathologies. Over the last few years, relevant investigations have elucidated that miRNAs participate in different stages of bone growth and development. Moreover, the abnormal expression of these RNA molecules in bone cells and tissues has been significantly associated with the progression of numerous bone diseases, including osteoporosis, osteosarcoma, osteonecrosis and bone metastasis, among others. In fact, miRNAs regulate multiple pathological mechanisms, including altering either osteogenic or osteoblast differentiation, metastasis, osteosarcoma cell proliferation, and bone loss. Therefore, in this present review, aiming to impulse the research arena of the biological implications of miRNA transcriptome in bone diseases and to explore their potentiality as a theragnostic target, we summarize the recent findings associated with the clinical significance of miRNAs in these ailments.

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Section: Osteoporosis and Mirnasmentioning

confidence: 99%

Section: Osteoporosis and Mirnasmentioning

confidence: 99%

The Emerging Role of MicroRNAs in Bone Diseases and Their Therapeutic Potential

et al. 2021

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“…Comparison between osteoporotic patients and controls [ 16 , 20 , 22 , 24 , 27 , 29 , 31 , 32 , 34 , 35 , 36 , 40 , 41 , 44 , 45 , 47 , 50 , 51 , 52 , 53 , 55 , 59 , 61 , 63 ]…”

Section: Serum Mirnas As Clinical Potential Biomarkers For Human Ostementioning

confidence: 99%

Application of microRNA in Human Osteoporosis and Fragility Fracture: A Systemic Review of Literatures

Huang

Cheng

et al. 2021

IJMS

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MicroRNAs (miRNAs) could serve as ideal entry points to the deregulated pathways in osteoporosis due to their relatively simple upstream and downstream relationships with other molecules in the signaling cascades. Our study aimed to give a comprehensive review of the already identified miRNAs in osteoporosis from human blood samples and provide useful information for their clinical application. A systematic literature search for relevant studies was conducted in the Pubmed database from inception to December 2020. We set two essential inclusion criteria: human blood sampling and design of controlled studies. We sorted the results of analysis on human blood samples according to the study settings and compiled the most promising miRNAs with analyzed diagnostic values. Furthermore, in vitro and in vivo evidence for the mechanisms of the identified miRNAs was also illustrated. Based on both diagnostic value and evidence of mechanism from in vitro and in vivo experiments, miR-23b-3p, miR-140-3p, miR-300, miR-155-5p, miR-208a-3p, and miR-637 were preferred candidates in diagnostic panels and as therapeutic agents. Further studies are needed to build sound foundations for the clinical usage of miRNAs in osteoporosis.

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“…MicroRNAs (miRNAs) naturally exist in a variety of living organisms, acting as a crucial element in gene modulation through binding to a specific region in open reading frames (ORFs) or untranslated regions (UTRs), blocking the translation process either by degrading or blocking the mRNA and leading to a knock down or through suppression of downstream processes 9 - 11 . It has been reported that overexpression of miR-29a can result in myocardial injury 12 .…”

Section: Introductionmentioning

confidence: 99%

SARS-CoV-2-induced Overexpression of miR-4485 Suppresses Osteogenic Differentiation and Impairs Fracture Healing

Mi¹,

Xiong²,

Zhang³

et al. 2021

Int. J. Biol. Sci.

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The angiotensin-converting enzyme 2 (ACE2) receptor has been identified as the cell entry point for SARS-CoV-2. Although ACE2 receptors are present in the bone marrow, the effects of SARS-CoV-2 on the biological activity of bone tissue have not yet been elucidated. In the present study we sought to investigate the impact of SARS-CoV-2 on osteoblastic activity in the context of fracture healing. MicroRNA-4485 (miR-4485), which we found to be upregulated in COVID-19 patients, negatively regulates osteogenic differentiation. We demonstrate this effect both in vitro and in vivo . Moreover, we identified the toll-like receptor 4 (TLR-4) as the potential target gene of miR-4485, and showed that reduction of TLR-4 induced by miR-4485 suppresses osteoblastic differentiation in vitro . Taken together, our findings highlight that up-regulation of miR-4485 is responsible for the suppression of osteogenic differentiation in COVID-19 patients, and TLR-4 is the potential target through which miR-4485 acts, providing a promising target for pro-fracture-healing and anti-osteoporosis therapy in COVID-19 patients.

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Inhibition of Circulating miR-194-5p Reverses Osteoporosis through Wnt5a/β-Catenin-Dependent Induction of Osteogenic Differentiation

Cited by 21 publications

References 25 publications

The Emerging Role of MicroRNAs in Bone Diseases and Their Therapeutic Potential

The Emerging Role of MicroRNAs in Bone Diseases and Their Therapeutic Potential

Application of microRNA in Human Osteoporosis and Fragility Fracture: A Systemic Review of Literatures

SARS-CoV-2-induced Overexpression of miR-4485 Suppresses Osteogenic Differentiation and Impairs Fracture Healing

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