Inhibition of CI857-controlled recombinant gene expression in Escherichia coli at very low concentrations of glucose

Ammonium‐Mediated Reduction of Plasmid Copy Number and Recombinant Gene Expression in Escherichia coli

“…Apart from the precise supplements required for a specific biological system or strain, the general influences of some medium components on recombinant gene expression have been described and studied. Among them, glucose-mediated inhibition of cell growth (Andersen and von Meyenburg, 1980) and product yield (Rinas et al, 1989;Vila and Villaverde, 1993) is of relevant interest because this sugar is often employed as a carbon source in defined media or even as a supplement in complex media. In general, all of the essential substrates for bacterial growth are toxic to the cells beyond critical thresholds, which are specific for each particular strain, medium, and growth conditions.…”

“…The results presented are the mean of four independent experiments. Absolute values of @-galactosidase activity during typical induction experiments in LB can be found elsewhere(Vila and Villaverde, 1993).…”

High‐Level Expression of a Heterologous Gene in Escherichia coli in Response to Carbon‐Nitrogen Source and C/N Ratio in a Batch Bioreactor

“…Li and Taylor (1994) have reported improvement in expression parameters of cloned gene upon employing glucose feed at a rate of 2 g L -1 in a complex medium, LB. However, inhibitory effects of glucose on the production of recombinant protein by E. coli in complex medium in batch bioreactor have been frequently observed (Jensen and Carlsen, 1990;Rinas et al, 1989;Vila and Villeverde, 1993). Such an effect was consequence of accumulation of acetate at inhibitory levels which could be avoided by modulating the glucose uptake rate (Chou et al, 1994) or by using an alternative substrate (Kwon et al, 1996).…”