Inhibition of Chronic Ulcerative Colitis-associated Adenocarcinoma Development in Mice by VSL#3

Abstract

VSL#3 administration reduced chronic inflammation and prevented or delayed the development of dysplasia and carcinoma in a mouse model of chronic colitis-associated cancer.

Evidence of the Anti-Inflammatory Effects of Probiotics and Synbiotics in Intestinal Chronic Diseases

“…A probiotic mixture attenuated the disease activity index score and colon inflammation after 5, 10, and 15 cycles of DSS and reduced the histological alterations and the incidence of colonic dysplastic lesions in the three periods studied. In addition, the probiotic reduced the proliferating cell nuclear antigen labeling index and TNF-α, IL-1β, IL-6 production, and cyclooxygenase (COX)-2 expression, and increased IL-10 levels in colon tissue in the three periods assayed [49]. Additionally, in rats treated with DSS for seven days, the probiotic mixture exhibited anti-inflammatory properties, including reducing the disease activity index, MPO activity, iNOS, COX-2, NF-κB, TNF-α, IL-6, and p-Akt expression, and increasing IL-10 expression in colonic tissue.…”

The Algal Meroterpene 11-Hydroxy-1′-O-Methylamentadione Ameloriates Dextran Sulfate Sodium-Induced Colitis in Mice

“…All tissue sections were examined in an Olympus BH-2 microscope (GMI, Ramsey, MN, USA) for characterization of histopathology changes. The tissues were analysed by a blinded observer to establish a composite Histological Score as previously described [6], where researchers do not know the origin of the sample, except the coordinator. Criteria included mucosal architecture (0, absent; 1, mild; 2, medium; 3, severe), cellular infiltration (0, none; 1, infiltrate around the crypt basis; 2, infiltrate reaching the muscularis mucosae; 3, infiltrate reaching the submucosa), and goblet cell depletion (0, absent; 1, present).…”

“…Various models of experimental IBD have been developed to investigate the pathogenesis of this disease and may be used to test innovative approaches for therapy [5]. Among these models, experimental colitis induced by dextran sulphate sodium (DSS) has been widely used because it affords a high degree of uniformity and reproducibility of most lesions in the distal colon, and after 7 days of DSS administration the intestinal immune system is activated to generate, in a coordinated form, pro-inflammatory/anti-inflammatory cytokines [6]. The pro-inflammatory cytokines amplify the inflammatory response by activating a cascade of immune cells such as neutrophils and macrophages.…”

Expression patterns of sirtuin 1-AMPK-autophagy pathway in chronic colitis and inflammation-associated colon neoplasia in IL-10-deficient mice

“…Negative control sections were treated in the same way, omitting the primary antibody. The proliferation index was determined by counting the number of PCNA immunostained-positive cells among at least 200 cells in the lesion, indicated as percentages [18].…”