Inhibition of cell spreading by lysosomotropic amines

Seglen, Per Ottar; Gordon, Paul B.

doi:10.1016/0014-5793(79)80645-6

Cited by 24 publications

(17 citation statements)

References 31 publications

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“…119 All lysosomotropic amines will, however, induce swelling of lysosomes and other acidic compartments, thus interfering with cellular processes such as endocytosis, 125 secretion 126 and even locomotion. 127 Genetic knockouts and knockdowns. Because the ATG genes are essential for autophagy in yeast, mutational knockouts of these genes will not only affect the individual step regulated by the respective protein, but will also usually block the whole autophagiclysosomal (vacuolar) pathway.…”

Section: Inhibition Of Autophagymentioning

confidence: 99%

Methods for Monitoring Autophagy from Yeast to Human

2007

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The increasing interest in autophagy in a wide range of organisms, accompanied by an ever-growing influx of researchers into this field, necessitates a good understanding of the methodologies available to monitor this process. In this review we discuss current approaches that can be used to follow the overall process of autophagy, as well as individual steps, from yeast to human. The majority of the review considers methods that apply to macroautophagy; however, we also consider alternative types of degradation including chaperone-mediated autophagy and microautophagy. This information is meant to provide a resource for newcomers as well as a stimulus for experienced researchers who may be prompted to develop additional assays to examine autophagy-related pathways.

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Section: Inhibition Of Autophagymentioning

confidence: 99%

Methods for Monitoring Autophagy from Yeast to Human

2007

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“…Polyamines have been reported to inhibit acidic and neutral proteases in oat leaves [5] and neutral proteases in alfalfa leaf extracts [6]. In isolated hepatocytes, polyamines did not affect the rate of degradation of proteins within the lysosome [7]. In reticulocytes, many cell proteins are degraded during maturation by a nonlysosomal proteolytic pathway that requires metabolic energy [8].…”

Section: Introductionmentioning

confidence: 99%

Polyamines inhibit the ATP‐dependent proteolytic pathway in rabbit reticulocyte lysates

Wajnberg¹,

Fagan²

1989

FEBS Letters

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Reticulocytes contain a soluble nonlysosomal proteolytic pathway that requires ATP and ubiquitin. Polyamines at physiological concentrations were found to inhibit rapidly the ATP-dependent proteolytic system in reticulocyte lysates; spcrmidine and putrescine inhibited this process by 26-72% and spermine by 71-96~. Spermine had little effect on the ATP-independent breakdown of oxidant-treated hemoglobin. By fractionating the ATP-dependent system, we show that polyamines inhibit the ATP-dependent degradation of ubiquitin-protein conjugates.

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“…It has been shown that endosomal acidification is essential for parvovirus infection, triggering capsid structural rearrangements and escape of the virus from endosomes into the cytoplasm (13,34,38,41,53). To determine the role of a low-pH-dependent step in JcDNV infectivity, we examined the effects on infection of two weak bases known to raise the pH in endosomes and lysosomes, CQ and ammonium chloride (NH 4 Cl) (35,43). Thirty minutes after treatments, viral staining appeared diffuse in both types of treated cells compared to the control, which might be due to the swelling of acidic vesicles (Fig.…”

Section: Resultsmentioning

confidence: 99%

Densovirus Infectious Pathway Requires Clathrin-Mediated Endocytosis Followed by Trafficking to the Nucleus

Vendeville

Ravallec

Jousset

et al. 2009

J Virol

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Junonia coenia densovirus (JcDNV) is an ambisense insect parvovirus highly pathogenic for lepidopteran pests at larval stages. The potential use of DNVs as biological control agents prompted us to reinvestigate the host range and cellular mechanisms of infection. In order to understand the early events of infection, we set up a functional infection assay in a cell line of the pest Lymantria dispar to determine the intracellular pathway undertaken by JcDNV to infect a permissive lepidopteran cell line. Our results show that JcDNV particles are rapidly internalized into clathrin-coated vesicles and slowly traffic within early and late endocytic compartments. Blocking late-endocytic trafficking or neutralizing the pH with drugs inhibited infection. During internalization, disruption of the cytoskeleton, and inhibition of phosphatidylinositol 3-kinase blocked the movement of vesicles containing the virus to the nucleus and impaired infection. In summary, our results define for the first time the early endocytic steps required for a productive DNV infection.

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Inhibition of cell spreading by lysosomotropic amines

Cited by 24 publications

References 31 publications

Methods for Monitoring Autophagy from Yeast to Human

Methods for Monitoring Autophagy from Yeast to Human

Polyamines inhibit the ATP‐dependent proteolytic pathway in rabbit reticulocyte lysates

Densovirus Infectious Pathway Requires Clathrin-Mediated Endocytosis Followed by Trafficking to the Nucleus

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