Inhibition of cell proliferation, migration and invasion of B16-F10 melanoma cells by α-mangostin

Beninati, Simone; Oliverio, Serafina; Cordella, Martina; Rossi, Stefania; Senatore, Cinzia; Liguori, Immacolata; Lentini, Alessandro; Piredda, Lucia; Tabolacci, Claudio

doi:10.1016/j.bbrc.2014.07.031

Cited by 31 publications

(19 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these compounds, α -mangostin was shown to have the strongest apoptotic activity in human melanoma cells and, compared to other similar compounds, α -mangostin was shown to be the most effective agent in human cancer cell lines [ 27 , 28 ]. α -Mangostin has also been reported to act as an anticancer agent against various types of cancer cells including canine osteosarcoma cells, human colorectal cancer cells, and pancreatic cancer cells [ 6 , 29 – 32 ]. This suggests that α -mangostin may be considered a prospective anticancer agent; however, the biological mechanism underlying α -mangostin-induced cell death in human OSCC cell lines has not been well studied.…”

Section: Discussionmentioning

confidence: 99%

α‐Mangostin Induces Apoptosis and Cell Cycle Arrest in Oral Squamous Cell Carcinoma Cell

Kwak

Kim

et al. 2016

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Mangosteen has long been used as a traditional medicine and is known to have antibacterial, antioxidant, and anticancer effects. Although the effects of α-mangostin, a natural compound extracted from the pericarp of mangosteen, have been investigated in many studies, there is limited data on the effects of the compound in human oral squamous cell carcinoma (OSCC). In this study, α-mangostin was assessed as a potential anticancer agent against human OSCC cells. α-Mangostin inhibited cell proliferation and induced cell death in OSCC cells in a dose- and time-dependent manner with little to no effect on normal human PDLF cells. α-Mangostin treatment clearly showed apoptotic evidences such as nuclear fragmentation and accumulation of annexin V and PI-positive cells on OSCC cells. α-Mangostin treatment also caused the collapse of mitochondrial membrane potential and the translocation of cytochrome c from the mitochondria into the cytosol. The expressions of the mitochondria-related proteins were activated by α-mangostin. Treatment with α-mangostin also induced G1 phase arrest and downregulated cell cycle-related proteins (CDK/cyclin). Hence, α-mangostin specifically induces cell death and inhibits proliferation in OSCC cells via the intrinsic apoptosis pathway and cell cycle arrest at the G1 phase, suggesting that α-mangostin may be an effective agent for the treatment of OSCC.

show abstract

Section: Discussionmentioning

confidence: 99%

α‐Mangostin Induces Apoptosis and Cell Cycle Arrest in Oral Squamous Cell Carcinoma Cell

Kwak

Kim

et al. 2016

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…Our chemical study indicated the presence of various kinds of polyphenols and flavonoids [chlorogenic acid, (− )-EC, rutin, hyperoside, vitexin-2″-O-rhamnoside, procyanidin B2, and procyanidin C1] and triterpenes (oleanolic acid and ursolic acid) and as they were described previously for their antiproliferative activity against melanoma cells [7,[23][24][25][26][27][28], we believe that they are responsible at least in part for the antiproliferative potential of TOF extract. This finding is in good accordance with our previous studies that showed how C. azarolus ethyl acetate extract exerted a potent viability inhibition effect of high-metastasic B16F10 [7].…”

Section: Discussionmentioning

confidence: 91%

Antitumoral, antioxidant, and antimelanogenesis potencies of Hawthorn, a potential natural agent in the treatment of melanoma

et al. 2016

View full text Add to dashboard Cite

The lack of an efficient agent that does not have the disadvantage of low activity (kojic acid), high cytotoxicity, and mutagenicity (hydroquinone), poor skin penetration (arbutin), or low stability in formulation (glabridin) led us to continue our research on new antipigmentation/skin-lightening agents. Therefore, research of natural products that can modulate the metabolism of pigmentation is of great interest. Otherwise, malignant melanoma is one of the most aggressive forms of skin cancer, with high metastatic potential, and currently, there is no effective chemotherapy against invasive melanoma. Therefore, it is necessary to develop new drugs with potent activity and weak side effects against melanoma. The in-vitro anticancer effect of hawthorn was analyzed against B16F10 melanoma cells using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The effect of isolated compounds from hawthorn on melanogenesis in B16F10 melanoma cells was investigated by measuring the amounts of melanin and tyrosinase spectrophotometrically at 475 nm. Balb/c mice models inoculated with B16F10 mouse tumor cells were used to evaluate the in-vivo antitumoral potential of hawthorn by assessing its effect on the growth of transplanted tumors. The antioxidant potential of tested samples was evaluated in B16F10 and primary human keratinocyte cells using a cellular antioxidant activity assay. Hawthorn tested samples inhibited effectively the growth of melanoma cells in vitro. Furthermore, it appears that tested samples from hawthorn reduced melanogenesis by inhibiting the tyrosinase activity of B16F10 cells in a dose-dependent manner. In-vivo studies showed that hawthorn total oligomer flavonoids extract treatment at a dose of 150 mg/kg body weight for 21 days in implanted tumor mice resulted in significant inhibition of the tumor growth volume and weight. In addition, tested samples showed significant cellular antioxidant capacity against the reactive oxygen species in B16F10 and primary human keratinocyte cells. Our results indicate that hawthorn could be considered as a promising agent for the treatment of melanoma as it shows antitumor activity in vitro and in vivo. Moreover, hawthorn constituents are shown to be highly effective at inhibiting tyrosinase-mediated melanogenesis in vitro on melanoma cells by preventing oxidation in these cells and without affecting the viability of normal human keratinocyte cells. Then, hawthorn might also be used as a new candidate of natural skin depigmenting agents in skin care products.

show abstract

“…In our previous study, α -mangostin was demonstrated to have a potent anticancer effect in human oral squamous cell carcinoma cell lines (OSCC) through apoptosis and cell cycle arrest [ 19 ]. Also, α -mangostin has been reported to inhibit migration and invasion in various cancer cells, including melanoma cells, pancreatic cancer cells, and lung adenocarcinoma cells [ 17 , 29 – 31 ]. However, the effect of α -mangostin on human osteosarcoma cells is uncertain.…”

Section: Discussionmentioning

confidence: 99%

Induction of Apoptosis and Inhibition of Epithelial Mesenchymal Transition by α‐Mangostin in MG‐63 Cell Lines

Park

et al. 2018

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Osteosarcoma is the most common bone primary malignant tumor and nearly 30% of patients still die from osteosarcoma due to metastasis or recurrence. Thus, it is necessary to develop effective new chemotherapeutic agents for osteosarcoma treatment. α-Mangostin is a xanthone derivative shown to have antioxidant and anticarcinogen properties. However, the molecular mechanisms underlying the antimetastatic effects of osteosarcoma remain unclear. In metastasis progression, epithelial mesenchymal transition (EMT) is a process that plays important roles in development, cell polarity, and increased invasion and migration. This study focused on the induction of apoptosis and inhibition of EMT process by α-mangostin in human osteosarcoma cell line MG63. α-Mangostin treatments on MG63 cells not only showed the several lines of evidence of apoptotic cell death but also inhibited cell migration, invasion, and EMT-inducing transcription factor. In conclusion, we demonstrate that the α-mangostin induces apoptosis via mitochondrial pathway and suppresses metastasis of osteosarcoma cells by inhibiting EMT.

show abstract

Inhibition of cell proliferation, migration and invasion of B16-F10 melanoma cells by α-mangostin

Cited by 31 publications

References 34 publications

α‐Mangostin Induces Apoptosis and Cell Cycle Arrest in Oral Squamous Cell Carcinoma Cell

α‐Mangostin Induces Apoptosis and Cell Cycle Arrest in Oral Squamous Cell Carcinoma Cell

Antitumoral, antioxidant, and antimelanogenesis potencies of Hawthorn, a potential natural agent in the treatment of melanoma

Induction of Apoptosis and Inhibition of Epithelial Mesenchymal Transition by α‐Mangostin in MG‐63 Cell Lines

Contact Info

Product

Resources

About