Inhibition of cell invasion and protease activity by cholesterol sulfate

Nakae, Hanako; Hiroi, Hisahiko; Momoeda, Mikio; Koizumi, Minako; Iwamori, Masao; Taketani, Yuji

doi:10.1016/j.fertnstert.2010.02.012

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…Gelatin zymography analysis indicated inhibition of plasmin‐mediated activation of MMP‐9 by 10 μM SC . In another study, Nakae et al showed that SC directly inhibited plasmin activity in a noncompetitive manner, which led to reduction of invasion of a trophoblast cell line . In combination, the results appear to indicate that SC inhibition of plasmin may be essential for regulating the process of embryo implantation.…”

Section: Plasmin Inhibitors For Other Diseasesmentioning

confidence: 97%

“…41 Plasmin also contributes indirectly to ECM degradation by its activation of matrix metalloproteinases (MMPs), which degrade other ECM components. 38,[42][43][44] In fact, potential benefits of plasmin inhibition have been projected for angioedema, 45,46 chronic inflammatory responses, 47,48 embryogenesis, 37,49 and lymphoid malignancies. 50,51 Thus, plasmin inhibitors may also be of value as anticancer and antiinflammatory agents in addition to their well-known therapeutic use as antifibrinolytic agents.…”

Section: Introductionmentioning

confidence: 99%

“…50,51 Thus, plasmin inhibitors may also be of value as anticancer and antiinflammatory agents in addition to their well-known therapeutic use as antifibrinolytic agents. [45][46][47][48][49][50][51] In this review, the plasminogen-plasmin system and its central physiological role in the fibrinolytic pathway will be discussed in an attempt to understand fundamental rationale for plasmin inhibitors design and use. Recent advances made on the design, discovery, and development of plasmin inhibitors as antifibrinolytic agents will be more elaborately discussed.…”

Section: Introductionmentioning

confidence: 99%

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Recent Advances on Plasmin Inhibitors for the Treatment of Fibrinolysis‐Related Disorders

Al‐Horani

Desai

2014

Medicinal Research Reviews

101

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Growing evidence suggests that plasmin is involved in a number of physiological processes in addition to its key role in fibrin cleavage. Plasmin inhibition is critical in preventing adverse consequences arising from plasmin overactivity, e.g., blood loss that may follow cardiac surgery. Aprotinin was widely used as an antifibrinolytic drug before its discontinuation in 2008. Tranexamic acid and ε-aminocaproic acid, two small molecule plasmin inhibitors, are currently used in the clinic. Several molecules have been designed utilizing covalent, but reversible, chemistry relying on reactive cyclohexanones, nitrile warheads, and reactive aldehyde peptidomimetics. Other major classes of plasmin inhibitors include the cyclic peptidomimetics and polypeptides of the Kunitz and Kazal-type. Allosteric inhibitors of plasmin have also been designed including small molecule lysine analogs that bind to plasmin's kringle domain(s) and sulfated glycosaminoglycan mimetics that bind to plasmin's catalytic domain. Plasmin inhibitors have also been explored for resolving other disease states including cell metastasis, cell proliferation, angiogenesis, and embryo implantation. This review highlights functional and structural aspects of plasmin inhibitors with the goal of advancing their design.

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Section: Plasmin Inhibitors For Other Diseasesmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Recent Advances on Plasmin Inhibitors for the Treatment of Fibrinolysis‐Related Disorders

Al‐Horani

Desai

2014

Medicinal Research Reviews

101

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“…39,40) It activates factor XII, triggering the intrinsic blood coagulation system. On the other hand, it inhibits thrombin and plasmin, suppressing blood clotting.…”

Section: Mechanism Of Stabilization Of Mmp-7 By Heparinmentioning

confidence: 99%

Effects of heparin and cholesterol sulfate on the activity and stability of human matrix metalloproteinase 7

Samukange

Yasukawa

Inouye

2014

Bioscience, Biotechnology, and Biochemistry

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Sulfated glycosaminoglycans and sulfated lipids are involved in the biological functions of human matrix metalloproteinase 7 (MMP-7). In this study, the effects of heparin and cholesterol sulfate (CS) on the activity and stability of MMP-7 in the hydrolysis of a synthetic substrate, (7-methoxycoumarin-4-yl)were examined. Heparin increased activity by decreasing K m , and the K m values for 0 and 50 μM heparin were 57 ± 8 and 19 ± 5 μM, respectively. CS decreased activity in a non-competitive inhibitory manner with a K i value of 11 ± 3 μM. In thermal incubation at 50−70°C, heparin increased relative activity (the ratio of k cat /K m of MMP-7 with incubation to that without it), while CS decreased relative activity. These results indicate that heparin increases the activity and stability of MMP-7, while CS decreases them.

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“…CS plays a role in cellular membrane stabilization, regulation of the skin barrier, and steroid genesis [5][6][7]. Recently, we reported the inhibitory effect of proteases on CS in human endometrium [8,9]. CS was demonstrated to be a natural ligand of the retinoic acid receptor-related orphan receptor alpha (RORA) [10,11].…”

Section: Introductionmentioning

confidence: 99%

Expression of retinoic acid-related orphan receptor alpha and its responsive genes in human endometrium regulated by cholesterol sulfate

Zenri

Hiroi

Momoeda

et al. 2012

The Journal of Steroid Biochemistry and Molecular Biology

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Inhibition of cell invasion and protease activity by cholesterol sulfate

Cited by 6 publications

References 21 publications

Recent Advances on Plasmin Inhibitors for the Treatment of Fibrinolysis‐Related Disorders

Recent Advances on Plasmin Inhibitors for the Treatment of Fibrinolysis‐Related Disorders

Effects of heparin and cholesterol sulfate on the activity and stability of human matrix metalloproteinase 7

Expression of retinoic acid-related orphan receptor alpha and its responsive genes in human endometrium regulated by cholesterol sulfate

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