Inhibition of cell cycle progression on HepG2 cells by hypsiziprenol A9, isolated from Hypsizigus marmoreus

Chang, Jong-Sun; Son, Jong‐Keun; Gao, Li; Oh, Eun-Jung; Kim, Jiyoung; Park, Sun-Hee; Bae, Jang–Ho; Kim, Hyun-Jeong; Lee, In-Seon; Kim, Ok-Mi; Kozukue, Nobuyuki; Han, Jae-Sook; Hirose, Masao; Lee, Kap-Rang

doi:10.1016/j.canlet.2004.03.013

Cited by 28 publications

(19 citation statements)

References 28 publications

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“…The activities of two types of cyclins (cyclin D and cyclin E), Cdks (cyclin-dependent kinases 2, 4 and 6), and Cdks inhibitors are necessary for entering from the G1 phase into S phase of the cell cycle (Weinberg, 1989). Thus, most of bioactive substances from natural products have been reported to exert their anticancer activity by blocking cell cycle progression and triggering tumor cell apoptosis (Chang et al, 2004;Ye et al, 2005;Hsieh et al, 2006). Youn et al (2008) confirmed that I. obliquus water extract (IWE) previously used for treating cancers and digestive system diseases could selectively and significantly inhibited the viability and proliferation, and induced apoptotic cell death in human hepatoma HepG2 cells, while this effect was not found in the human immortalized non-tumor cell line.…”

Section: Involving In the Arrest Of Cell Cycle In Cancer Cellsmentioning

confidence: 80%

Progress on Understanding the Anticancer Mechanisms of Medicinal Mushroom: Inonotus Obliquus

Song¹,

Liu²,

Kong³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Section: Involving In the Arrest Of Cell Cycle In Cancer Cellsmentioning

confidence: 80%

Progress on Understanding the Anticancer Mechanisms of Medicinal Mushroom: Inonotus Obliquus

Song¹,

Liu²,

Kong³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Tumor suppressor gene p53 has been shown to play an important role in apoptosis induction (Chiu et al, 2006). In addition, p21 has been known to play a critical role in the G 1 to S phase cell cycle transition (Chang et al, 2004b). We evaluated the mRNA and protein expressions of p53 and p21 in CaSki cells.…”

Section: Effects Of Pe On Apoptosis-related Genes By Rt-pcr and Westementioning

confidence: 99%

HPV E6 down-regulation and apoptosis induction of human cervical cancer cells by a novel lipid-soluble extract (PE) from Pinellia pedatisecta Schott in vitro

Jiang

Xia

et al. 2010

Journal of Ethnopharmacology

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“…It has been reported to exhibit many biological activities such as anti-tumor, 1,2) antioxidant, 1) and antiproliferative activity. 3) Several homologous polyisoprenepolyols named hypsiziprenols have recently been characterized in this mushroom. 4) Hypsiziprenols are the possible biosynthetic precursors of gymnopilins, bitter principles of H. marmoreus.…”

mentioning

confidence: 99%

Antitubercular Activity and Inhibitory Effect on Epstein-Barr Virus Activation of Sterols and Polyisoprenepolyols from an Edible Mushroom, Hypsizigus marmoreus

Akihisa

Franzblau

Tokuda

et al. 2005

Biological & Pharmaceutical Bulletin

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Hypsizigus marmoreus (Buna-shimeji) has recently become a popular edible mushroom in Asia, and has been cultivated by culture methods such as bottle culture and bag-culture. It has been reported to exhibit many biological activities such as anti-tumor, 1,2) antioxidant, 1) and antiproliferative activity. 3) Several homologous polyisoprenepolyols named hypsiziprenols have recently been characterized in this mushroom. 4) Hypsiziprenols are the possible biosynthetic precursors of gymnopilins, bitter principles of H. marmoreus. 4) In the course of our studies on the bioactive principles of natural medicines and foodstuffs, 5,6) we evaluated the constituents of H. marmoreus for their antitubercular activity 7) as well as their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). 5,6) In this paper, we describe the antitubercular activity against Mycobacterium tuberculosis of seven sterols (1-7) and eight polyisoprenepolyols, hypsiziprenols (8-15), and inhibitory effects on the induction of EBV-EA by the TPA of seven sterols (1-7) and three hypsiziprenols (8, 10, 12) isolated from the non-saponifiable lipid (NSL) fraction of the extract of H. marmoreus. MATERIALS AND METHODSExtraction, Isolation, and Identification Sliced fresh bodies of Hypsizigus marmoreus (11 kg), cultivated in Nagano Prefecture in 2001, were air-dried. The dried mushroom (833 g) was then extracted with hot CH 2 Cl 2 to give an extract (86 g). Alkaline hydrolysis (5% KOH in methanol (MeOH), reflux, 3 h) of the extract yielded a neutral nonsaponifiable lipid (NSL) fraction (9.0 g). Crystallization of the NSL fraction from MeOH yielded a precipitate (2.8 g), which was identified as ergosterol (1), 2) and a filtrate (4.9 g).The filtrate was subjected to column chromatography on silica gel (150 g) using an n-hexane-ethyl acetate (EtOAc) gradient of 1 : 0-1 : 1 as an eluent, which yielded fractions containing monohydroxy sterols (fraction A; Rf 0.83 on TLC developed with EtOAc-MeOH, 4 : 1), polyhydroxy sterols (fraction B; Rf 0.48), and polyisoprenepolyols (fraction C; Rf 0.24). Fractions A-C were purified by further chromatography on silica gel. Reversed-phase preparative HPLC (HPLC I; 25 cmϫ10 mm i.d., Pegasil ODS II (Senshu Scientific Co., Ltd., Tokyo, Japan) column; mobile phase: MeOH-H 2 O- Farmers' Federation; 787-1 Suzaka, Sazaka, Nagano 382-0084, Japan: and e College of Pharmacy, Nihon University; 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan. Received January 18, 2005; accepted February 28, 2005 Seven sterols (1-7) and eight polyisoprenepolyols (8-15), isolated from the non-saponifiable lipid fraction of the dichloromethane extract of an edible mushroom, Hypsizigus marmoreus (Buna-shimeji), were tested for their antitubercular activity against Mycobacterium tuberculosis strain H 37 Rv using the Microplate Alamar Blue Assay (MABA). Six sterols (2-7) and two polyisoprenepolyols (8, 12) showed a minimum inhibitory concentration (MIC) in the range of 1-51 m mg...

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Inhibition of cell cycle progression on HepG2 cells by hypsiziprenol A9, isolated from Hypsizigus marmoreus

Cited by 28 publications

References 28 publications

Progress on Understanding the Anticancer Mechanisms of Medicinal Mushroom: Inonotus Obliquus

Progress on Understanding the Anticancer Mechanisms of Medicinal Mushroom: Inonotus Obliquus

HPV E6 down-regulation and apoptosis induction of human cervical cancer cells by a novel lipid-soluble extract (PE) from Pinellia pedatisecta Schott in vitro

Antitubercular Activity and Inhibitory Effect on Epstein-Barr Virus Activation of Sterols and Polyisoprenepolyols from an Edible Mushroom, Hypsizigus marmoreus

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