Inhibition of cell cycle progression in human leukemia HL-60 cells by esculetin

Wang, Chau Jong; Hsieh, Yu Jin; Chu, Chia-Yih; Lin, Yu; Tseng, Tsui Hwa

doi:10.1016/s0304-3835(02)00031-9

Cited by 257 publications

(99 citation statements)

References 21 publications

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“…An increase in CDK inhibitors p21 and p27 was also observed. Treatment of human leukaemia HL-60 cell with dihydroxycoumarin esculetin was also associated with a G 1 phase block in a study by Wang et al (2002). Decreased levels of cyclin D1 and E, CDK 4 and hyperphosphorylated retinoblastoma protein were observed as well as increased levels of p27.…”

Section: Discussionmentioning

confidence: 53%

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Pereira

Bester

Soundy

et al. 2016

Pharmaceutical Biology

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Context Pelargonium sidoides DC (Geraniaceae) is an important medicinal plant indigenous to South Africa and Lesotho. Previous studies have shown that root extracts are rich in polyphenolic compounds with antibacterial, antiviral and immunomodulatory activities. Little is known regarding the anticancer properties of Pelargonium sidoides extracts. Objective This study evaluates the anti-proliferative effects of a Pelargonium sidoides radix mother tincture (PST). Materials and methods The PST was characterized by LC-MS/MS. Anti-proliferative activity was evaluated in the pre-screen panel of the National Cancer Institute (NCI-H460, MCF-7 and SF-268) and the Jurkat leukaemia cell line at concentrations of 0-150 mg/mL. The effect on cell growth was determined with sulphorhodamine B and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays after 72 h. The effect on cell cycle and apoptosis induction in Jurkat cells was determined by flow cytometry with propidium iodide and Annexin V: fluorescein isothiocyanate staining. Results Dihydroxycoumarin sulphates, gallic acid as well as gallocatechin dimers and trimers were characterized in PST by mass spectrometry. Moderate anti-proliferative effects with GI 50 values between 40 and 80 mg/mL were observed in the NCI-pre-screen panel. Strong activity observed with Jurkat cells with a GI 50 value of 6.2 mg/mL, significantly better than positive control 5-fluorouracil (GI 50 value of 9.7 mg/mL). The PST arrested Jurkat cells at the G 0 /G 1 phase of the cell cycle and increased the apoptotic cells from 9% to 21%, while the dead cells increased from 4% to 17%. Conclusion We present evidence that P. sidoides has cancer cell type-specific anti-proliferative effects and may be a source of novel anticancer molecules. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 53%

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Pereira

Bester

Soundy

et al. 2016

Pharmaceutical Biology

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“…Multiple biological activities are attributed to esculin such as the inhibition of xanthine oxidase activity, antioxidant activity, antitumor activity and an inhibitory effect on the growth of human breast cancer cells (Duncan, Flint, Stewart, 1998;Wang et al, 2002). Zhao et al (2007) also described anti-oxidative and anti-apoptotic properties of esculin, being proposed as an alternative to the treatment of progressive neurodegenerative diseases such as Parkinson's disease.…”

Section: Resultsmentioning

confidence: 99%

Guava: phytochemical composition of a potential source of antioxidants for cosmetic and/or dermatological applications

Chiari-Andréo

Trovatti

Marto

et al. 2017

Braz. J. Pharm. Sci.

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Guava (Psidium guajava L.) is a native fruit of the American tropics with commercial applications for its taste, flavor and aroma. Numerous pharmacological uses have been described for it, such as the antiseptic effect of its leaves, the use of the fresh fruit and tea from its leaves for the treatment of diarrhea, dysentery, diabetes mellitus, and others. However, considering its rich composition, the guava also is a potential source of antioxidants to be used in the development of new formulations for cosmetic and/or dermatological applications, the main focus of this research. Herein, we describe the study of the phytochemical composition and the antioxidant activity of a guava extract prepared with non-toxic solvents aiming its use at biological applications. High performance liquid chromatography and mass spectrometry were employed to identify the major components, while thermoanalytical measurements and hot stage microscopy were used to assess the chemical stability of guava fruit extract. The antioxidant activity was also evaluated assessing the SOD-like activity and ABTS free radical scavenger. The results show that the extract is a rich source of phenolic compounds, such as quercetin, kaempferol, schottenol, among many others. All of the components found in guava extract exhibit biological effects according to the literature data, mainly antioxidant properties. Uniterms

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“…The Knoevenagel condensation has often been used to prepare substituted coumarin, one of the most important chemicals in the cosmetics, agricultural, pharmaceutical and material industries for its bioactivity and photodynamic effect (Kostova et al, 1993;Palmer and Josephs, 1995;Mitra et al, 1998;Wang et al, 2002). Highly efficient reactions have been reported, involving the condensation of salicylaldehydes with malonates in the presence of piperidine under microwave conditions (Bogdal, 1998), and the use of montmorillonite KSF (Bigi et al, 1999) which requires high temperature and long reaction time (100 °C, 24 h).…”

Section: Introductionmentioning

confidence: 99%

L-Proline as an efficient and reusable promoter for the synthesis of coumarins in ionic liquid

Liu

Fan

Liu

et al. 2008

J. Zhejiang Univ. Sci. B

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Abstract:The effect of L-proline as a promoter on the condensation reaction of salicylaldehyde or its derivatives with ethyl acetoacetate in neutral ionic liquid [emim]BF 4 was studied. All reactions were carried out under mild reaction conditions and achieved high yields. Moreover, the ionic liquid containing L-proline could be recycled and reused for several times without noticeably decreasing in productivity. The results show that the L-proline-[emim]BF 4 system has a potential in contribution to the development of environmentally friendly and inexpensive processes in organic syntheses.

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Inhibition of cell cycle progression in human leukemia HL-60 cells by esculetin

Cited by 257 publications

References 21 publications

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Guava: phytochemical composition of a potential source of antioxidants for cosmetic and/or dermatological applications

L-Proline as an efficient and reusable promoter for the synthesis of coumarins in ionic liquid

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Inhibition of cell cycle progression in human leukemia HL-60 cells by esculetin

Cited by 257 publications

References 21 publications

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G0/G1 arrest and apoptosis in Jurkat leukaemia cells

Guava: phytochemical composition of a potential source of antioxidants for cosmetic and/or dermatological applications

L-Proline as an efficient and reusable promoter for the synthesis of coumarins in ionic liquid

Contact Info

Product

Resources

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Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells

Anti-proliferative properties of commercial Pelargonium sidoides tincture, with cell-cycle G₀/G₁ arrest and apoptosis in Jurkat leukaemia cells