Inhibition of CD146 gene expression via RNA interference reduces <i>in vitro</i> perineural invasion on ACC‐M cell

Chen, W.; Zhang, H. L.; Jiang, Yuguang; Li, J. H.; Liu, B. L.; Sun, Miao

doi:10.1111/j.1600-0714.2008.00706.x

Cited by 20 publications

(15 citation statements)

References 36 publications

(38 reference statements)

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“…Cells that had invaded the Matrigel and reached the lower surface of the filter were fixed in methanol, stained with H&E, and counted as previously described. 14 In vivo tumorigenicity assays. All protocols involving animals were approved by the China Institutional Ethics Review Committee for Animal Experimentation.…”

Section: Methodsmentioning

confidence: 99%

EMMPRIN silencing inhibits proliferation and perineural invasion of human salivary adenoid cystic carcinoma cells in vitro and in vivo

Yang

Zhang

et al. 2012

Cancer Biology & Therapy

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Salivary adenoid cystic carcinoma (SACC) is a frequent subtype of salivary gland malignancy, and it has an important biological behavior for perineural invasion (PNI). Extracellular matrix metalloproteinase inducer (EMMPRIN) is a transmembrane glycoprotein that is involved in the invasive property of many malignancies by stimulating matrix metalloproteinase (MMP) expression. The present study was designed to investigate the role and possible mechanism of EMMPRIN in the PNI of SACC using RNA interference (RNAi) technology. We found that silencing of EMMPRIN expression in the human SACC cell line (SACC-83) suppressed the cell proliferation, adhesion, MMP-2 and MMP-9 secretion, and PNI activity in vitro. EMMPRIN silencing also inhibited the tumor growth and K i -67 labeled proliferation index in vivo. Using tumor PNI models in nude mice, EMMPRIN silencing inhibited the infiltration, swelling and functional loss of the affected sciatic nerves, as well as the expression of MMP-2 and MMP-9. These results demonstrate that EMMPRIN participates in the PNI of SACC cells by mediating the expression of MMP-2 and MMP-9. Our findings suggest that EMMPRIN is a potential target for anti-PNI treatment in SACC.

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Section: Methodsmentioning

confidence: 99%

EMMPRIN silencing inhibits proliferation and perineural invasion of human salivary adenoid cystic carcinoma cells in vitro and in vivo

Yang

Zhang

et al. 2012

Cancer Biology & Therapy

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“…Ryschich et al identified expression of laminin in the perineural space of nerves in pancreatic cancer and increased migration and invasion of pancreatic cells on laminin substrates was observed during pancreatic cancer PNI (Ryschich et al, 2009). Recent work has also demonstrated that inhibition of melanoma cell adhesion molecule (MCAM or CD146) using short hairpin silencing RNA in an adenoid cystic carcinoma cell line decreased perineural invasion in vitro (Chen et al, 2009). …”

Section: Cell Adhesion Molecules and Perineural Invasionmentioning

confidence: 99%

The laminin binding integrin α6β1 in prostate cancer perineural invasion

Sroka

Anderson

McDaniel

et al. 2010

Journal Cellular Physiology

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Metastasizing prostate tumor cells invade along nerves innervating the encapsulated human prostate gland in a process known as perineural invasion. The extacellular matrix laminin class of proteins line the neural route and tumor cells escaping from the gland express the laminin binding integrin α6β1 as a prominent cell surface receptor. Integrin α6β1 promotes aggressive disease and supports prostate tumor cell metastasis to bone. Laminins and their integrin receptors are necessary for the development and maintenance of the peripheral nervous system, indicating the potential role for integrin receptors in directing prostate tumor cell invasion on nerves during perineural invasion.

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“…17 Various approaches for targeting MCAM have already been investigated; antibodies against MCAM, immunization with MCAM, silencing of Mcam expression with siRNA and shRNA molecules. 18,19,20,21,22,23,24,25,26,27 The use of antibodies against MCAM has been effective in vitro in the reduction of proliferation, migration and tube formation in human endothelial cells, 18,19 and invasion in melanoma and osteosarcoma cells. 19,20 The therapy of melanoma, osteosarcoma, hepatocarcinoma, leiomyosarcoma and pancreatic tumors in mice in vivo with intraperitoneal injection of antibodies against MCAM reduced tumor growth and the formation of metastases of melanoma and osteosarcoma.…”

Section: Introductionmentioning

confidence: 99%

“…It reduced proliferation or survival, adhesion, migration and invasion of cells. 22,23,24,25,26 There was only one in vivo study targeting MCAM mRNA by RNA interference technology (RNAi), where the treatment of pathological angiogenesis in mice with miRNA targeting MCAM mRNA significantly attenuated neovascularization. 27 A step further in this field would definitely be the use of plasmid DNA encoding shRNA against MCAM, which allows more stable and long-lasting expression of shRNA for the maximization of therapeutic effect in comparison with siRNA delivered alone.…”

Section: Introductionmentioning

confidence: 99%

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

Prosen

Markelc

Dolinšek

et al. 2014

Molecular Therapy - Nucleic Acids

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The melanoma cell adhesion molecule (MCAM) is involved in melanoma development and its progression, including invasiveness, metastatic potential and angiogenesis. Therefore, MCAM represents a potential target for gene therapy of melanoma, whose expression could be hindered with posttranscriptional specific gene silencing with RNA interference technology. In this study, we constructed a plasmid DNA encoding short hairpin RNA against MCAM (pMCAM) to explore the antitumor and antiangiogenic effects. The experiments were performed in vitro on murine melanoma and endothelial cells, as well as in vivo on melanoma tumors in mice. The antiproliferative, antimigratory, antiangiogenic and antitumor effects were examined after gene therapy with pMCAM. Gene delivery was performed by magnetofection, and its efficacy compared to gene electrotransfer. Gene therapy with pMCAM has proved to be an effective approach in reducing the proliferation and migration of melanoma cells, as well as having antiangiogenic effect in endothelial cells and antitumor effect on melanoma tumors. Magnetofection as a developing nonviral gene delivery system was effective in the transfection of melanoma cells and tumors with pMCAM, but less efficient than gene electrotransfer in in vivo tumor gene therapy due to the lack of antiangiogenic effect after silencing Mcam by magnetofection.

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Inhibition of CD146 gene expression via RNA interference reduces in vitro perineural invasion on ACC‐M cell

Abstract: These findings suggested that CD146 was an ACC-related gene and CD146 may play an important role in the carcinogenesis, progression and PNI activity of ACC.

Cited by 20 publications

References 36 publications

EMMPRIN silencing inhibits proliferation and perineural invasion of human salivary adenoid cystic carcinoma cells in vitro and in vivo

EMMPRIN silencing inhibits proliferation and perineural invasion of human salivary adenoid cystic carcinoma cells in vitro and in vivo

The laminin binding integrin α6β1 in prostate cancer perineural invasion

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

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