Inhibition of Cavin3 Degradation by the Human Parainfluenza Virus Type 2 V Protein Is Important for Efficient Viral Growth

Ohta, Keisuke; Matsumoto, Yusuke; Nishio, Machiko

doi:10.3389/fmicb.2020.00803

Cited by 4 publications

(3 citation statements)

References 47 publications

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“…92 Besides, Ohta and team found that the caveolae raft protein cavin-3 was increased due to hindrance of proteasomal degradation in hPIV-2 infected cells. 93 From a previous study by Mohan et al, it was reported that interaction of cavin-3 with cavin-1 and caveolin-1 increased caveolar surface dynamics. 94 Therefore, Ohta inferred that the binding of viral V protein to cavin-3 is responsible in stabilising the raft protein because V protein might have facilitated cavin-3-induced activation of caveolar dynamics, which in turn enhanced hPIV-2 assembly and budding.…”

Section: Modulation Of Lipid Rafts For Viral Assembly and Releasementioning

confidence: 96%

“…Cholesterol depletion by Gem and Lov impaired hPIV‐1 assembly and budding from infected cells by inhibiting lipid raft association of viral matrix (M) and nucleocapsid (N) proteins and also by interfering cell surface recruitment of envelope glycoproteins 92 . Besides, Ohta and team found that the caveolae raft protein cavin‐3 was increased due to hindrance of proteasomal degradation in hPIV‐2 infected cells 93 . From a previous study by Mohan et al., it was reported that interaction of cavin‐3 with cavin‐1 and caveolin‐1 increased caveolar surface dynamics 94 .…”

Section: Modulation Of Lipid Rafts For Viral Assembly and Releasementioning

confidence: 99%

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Viral modulation of lipid rafts and their potential as putative antiviral targets

Gee

Sea

Lal

2022

Reviews in Medical Virology

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Lipid rafts are ubiquitous in cells. They are identified as cholesterol and glycosphingolipid enriched microdomains on cellular membranes. They serve as platforms for cellular communications by functioning in signal transduction and membrane trafficking. Such structural organisation fulfils cellular needs for normal function, but at the same time increases vulnerability of cells to pathogen invasion. Viruses rely heavily on lipid rafts in basically every stage of the viral life cycle for successful infection. Various mechanisms of lipid rafts modification exploited by diverse viruses for attachment, internalisation, membrane fusion, genome replication, assembly and release have been brought to light. This review focuses on virus-raft interactions and how a wide range of viruses manipulate lipid rafts at distinct stages of infection. The importance of virus-raft interactions in viral infections has inspired researchers to discover and develop antivirals that target this interaction, such as statins, methyl-β-cyclodextrin, viperin, 25-hydroxycholesterol and even antimalarial drugs. The therapeutic modulations of lipid rafts as potential antiviral intervention from in vitro and in vivo evidence are discussed herein.

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Section: Modulation Of Lipid Rafts For Viral Assembly and Releasementioning

confidence: 96%

Section: Modulation Of Lipid Rafts For Viral Assembly and Releasementioning

confidence: 99%

Viral modulation of lipid rafts and their potential as putative antiviral targets

Gee

Sea

Lal

2022

Reviews in Medical Virology

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“…Besides, Cav-1 was clustered at sites of PIV-5 budding (Ravid et al, 2010). Human parainfluenza virus type 2 (hPIV-2) V protein bound to and stabilized cavin-3, which in turn promoted assembly and budding of hPIV-2 in lipid raft microdomains (Ohta et al, 2020).…”

Section: Mechanosensorsmentioning

confidence: 99%

How Physical Factors Coordinate Virus Infection: A Perspective From Mechanobiology

Liu

Tang

et al. 2021

Front. Bioeng. Biotechnol.

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Pandemics caused by viruses have threatened lives of thousands of people. Understanding the complicated process of viral infection provides significantly directive implication to epidemic prevention and control. Viral infection is a complex and diverse process, and substantial studies have been complemented in exploring the biochemical and molecular interactions between viruses and hosts. However, the physical microenvironment where infections implement is often less considered, and the role of mechanobiology in viral infection remains elusive. Mechanobiology focuses on sensation, transduction, and response to intracellular and extracellular physical factors by tissues, cells, and extracellular matrix. The intracellular cytoskeleton and mechanosensors have been proven to be extensively involved in the virus life cycle. Furthermore, innovative methods based on micro- and nanofabrication techniques are being utilized to control and modulate the physical and chemical cell microenvironment, and to explore how extracellular factors including stiffness, forces, and topography regulate viral infection. Our current review covers how physical factors in the microenvironment coordinate viral infection. Moreover, we will discuss how this knowledge can be harnessed in future research on cross-fields of mechanobiology and virology.

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Bioinformatics and next generation data analysis reveals the potential role of inflammation in sepsis and its associated complications

Vastrad¹,

Vastrad²

2023

Preprint

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Sepsis is the leading systemic inflammatory response syndrome in worldwide, yet relatively little is known about the genes and signaling pathways involved in sepsis progression. The current investigation aimed to elucidate potential key candidate genes and pathways in sepsis and its associated complications. Next generation sequencing (NGS) dataset (GSE185263) was downloaded from the Gene Expression Omnibus (GEO) database, which included data from 348 sepsis samples and 44 normal control samples. Differentially expressed genes (DEGs) were identified using t-tests in the DESeq2 R package. Next, we made use of the g:Profiler to analyze gene ontology (GO) and REACTOME pathway. Then protein-protein interaction (PPI) of these DEGs was visualized by Cytoscape with Search Tool for the Retrieval of Interacting Genes (STRING). Furthermore, we constructed miRNA-hub gene regulatory network and TF-hub gene regulatory network among hub genes utilizing miRNet and NetworkAnalyst online databases tool and Cytoscape software. Finally, we performed receiver operating characteristic (ROC) curve analysis of hub genes through the pROC package in R statistical software. In total, 958 DEGs were identified, of which 479 were up regulated and 479 were down regulated. GO and REACTOME results showed that DEGs mainly enriched in regulation of cellular process, response to stimulus, extracellular matrix organization and immune system. The hub genes of PRKN, KIT, FGFR2, GATA3, ERBB3, CDK1, PPARG, H2BC5, H4C4 and CDC20 might be associated with sepsis and its associated complications. Predicted miRNAs (e.g., hsa-mir-548ad-5p and hsa-mir-2113) and TFs (e.g., YAP1 and TBX5) were found to be significantly correlated with sepsis and its associated complications. In conclusion, the DEGs, relative pathways, hub genes, miRNA and TFs identified in the current investigation might help in understanding of the molecular mechanisms underlying sepsis and its associated complications progression and provide potential molecular targets and biomarkers for sepsis and its associated complications.

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Inhibition of Cavin3 Degradation by the Human Parainfluenza Virus Type 2 V Protein Is Important for Efficient Viral Growth

Cited by 4 publications

References 47 publications

Viral modulation of lipid rafts and their potential as putative antiviral targets

Viral modulation of lipid rafts and their potential as putative antiviral targets

How Physical Factors Coordinate Virus Infection: A Perspective From Mechanobiology

Bioinformatics and next generation data analysis reveals the potential role of inflammation in sepsis and its associated complications

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