Inhibition of cartilage degeneration and subchondral bone deterioration by <i>Spinacia oleracea</i> in human mimic of ACLT-induced osteoarthritis

Kothari, Priyanka; Sinha, Soumendu; Sardar, Anirban; Tripathi, Ashish Kumar; Girme, Aboli; Adhikary, Sulekha; Singh, Ruchi; Maurya, Rakesh; Mishra, Prabhat Ranjan; Hingorani, Lal; Trivedi, Ritu

doi:10.1039/d0fo01125h

Cited by 12 publications

(17 citation statements)

References 30 publications

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“…Cells were seeded and treated as above mentioned in complete alpha MEM media for 48 h. After the end of the experiment, MTT (5 mg/ml) was added to each well for 2–4 h. After the desired time DMSO was added to dissolve formazan crystal and absorbance was taken at 570 nm [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

“…To detect apoptosis in tissue we dewaxed and rehydrated bone tissue in Ethanol and water. Then processed according to manufacturer's protocol [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

“…We performed real-time PCR (Quant Studio 3, Applied Biosystems) for assessment of gene expression by SYBR green (PowerUp SYBR Green master mix Applied Biosystems). We checked gene expression of RUNX2, BMP2, ALP, β-catenin, SOST (Table 1 ) in response to dexamethasone and compound 4e [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

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Benzofuran pyran hybrid prevents glucocorticoid induced osteoporosis in mice via modulation of canonical Wnt/β-catenin signaling

et al. 2022

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Glucocorticoid induced osteoporosis (GIOP) is the second most leading cause of osteoporosis. We have identified a compound, a benzofuran pyran hybrid compound 4e that has osteogenic potential and we wanted to assess its efficacy in GIOP in male mice. We assessed the effect of dexamethasone and compound 4e on primary osteoblasts using various cell based and immunofluorescence assays. For in vivo studies we administered methylprednisolone and compound 4e as a prophylactic measure in male Balb/c mice for 28 days and then evaluated the effect on bone microarchitecture by microCT, bone formation by histology along with clinically relevant bone markers. Compound 4e preserved osteoblast differentiation as evident by higher ALP positive cells and mineralization in compound treated groups. Compound 4e also increased the expression of osteogenic genes. This compound guarded β-catenin expression both in vitro and in vivo as confirmed by western blot and immunofluorescence assays. This led to the preservation of bone microarchitecture and cortical thickness at 2.5 mg kg −1 and 5 mg kg −1 doses. Further compound 4e enhanced bone formation rate and regulated osteocyte death. The osteogenic potential of compound 4e was reflected by an increased level of serum marker osteocalcin and decreased levels of SOST and CTX-I. Overall, Compound 4e is able to overcome the catabolic effect of dexamethasone on bone by targeting the canonical WNT/β-catenin signaling as evidenced by both in vitro and in vivo studies.

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Section: Methodsmentioning

confidence: 99%

“…To detect apoptosis in tissue we dewaxed and rehydrated bone tissue in Ethanol and water. Then processed according to manufacturer's protocol [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

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Benzofuran pyran hybrid prevents glucocorticoid induced osteoporosis in mice via modulation of canonical Wnt/β-catenin signaling

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“…Osteoarthritis (OA) is a common chronic joint disease that is present globally. It has progressive articular cartilage degradation, affecting an increasing number of people in the elderly population, including the age group of 50–70 years ( 1 , 2 ). The pathogenesis of OA relates to complex interactions among multiple pathways, resulting in the loss of structural components of joints, inflammation, and senescence of chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Bioactive Compounds in Chicken Cartilage: Protective Effects of Chondroitin Sulfate and Type II Collagen Peptides Against Osteoarthritis Involve Gut Microbiota

Zhang

Shen

et al. 2022

Front. Nutr.

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This study was designed to explore osteoarthritis (OA) treatment from bioactive compounds of chicken cartilage food supplements. The OA rat model induced by sodium iodoacetate was used to evaluate the treatment effect in vivo. In this study, we used animal experiments to show that oral chondroitin sulfate (CS), cartilage powder, and type II collagen peptides could increase the athletic ability of rats and reduce inflammatory cytokine levels in serum or synovial fluid, including prostaglandin E2, tumor necrosis factor-α, interleukin (IL) 1β, IL-6, and IL-17. CS displayed the best treatment effect against OA. The morphological structure of articular cartilage indicated that CS could significantly improve cartilage tissue morphology and reduce OA score. Oral CS slowed down the development of OA by modulating gut microbiota. These results provided a useful scientific basis for the high-value utilization of chicken cartilage.

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“…In recent years, many researchers have begun to focus on the influence of the subchondral bone on the occurrence of KOA (12)(13)(14). The cartilage and subchondral bones are closely linked in the mechanical and biochemical microenvironment and are considered to be the key structural unit for maintaining the stability of the knee joint (15,16).…”

Section: Introductionmentioning

confidence: 99%

Intra-Articular Injection of Umbilical Cord Mesenchymal Stem Cells Loaded With Graphene Oxide Granular Lubrication Ameliorates Inflammatory Responses and Osteoporosis of the Subchondral Bone in Rabbits of Modified Papain-Induced Osteoarthritis

et al. 2022

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AimThis study is to investigate the effects of umbilical cord mesenchymal stem cells (UCMSCs) loaded with the graphene oxide (GO) granular lubrication on ameliorating inflammatory responses and osteoporosis of the subchondral bone in knee osteoarthritis (KOA) animal models.MethodsThe KOA animal models were established using modified papain joint injection. 24 male New Zealand rabbits were classified into the blank control group, GO group, UCMSCs group, and GO + UCMSCs group, respectively. The concentration in serum and articular fluid nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), type II collagen (COL-II), and glycosaminoglycan (GAG) was detected using ELISA, followed by the dissection of femoral condyles and staining of HE and Micro-CT for observation via the microscope.ResultsGO granular lubrication and UCMSCs repaired the KOA animal models. NO, IL-6, TNF-α, GAG, and COL-II showed optimal improvement performance in the GO + UCMSCs group, with statistical significance in contrast to the blank group (P <0.01). Whereas, there was a great difference in levels of inflammatory factors in serum and joint fluid. Micro-CT scan results revealed the greatest efficacy of the GO + UCMSCs group in improving joint surface damage and subchondral bone osteoporosis. HE staining pathology for femoral condyles revealed that the cartilage repair effect in GO + UCMSCs, UCMSCs, GO, and blank groups were graded down.ConclusionUCMSCs loaded with graphene oxide granular lubrication can promote the secretion of chondrocytes, reduce the level of joint inflammation, ameliorate osteoporosis of the subchondral bone, and facilitate cartilage repair.

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Inhibition of cartilage degeneration and subchondral bone deterioration by Spinacia oleracea in human mimic of ACLT-induced osteoarthritis

Abstract: Osteoarthritic conditions in ACLT model in rats were attenuated by SOE administration.

Cited by 12 publications

References 30 publications

Benzofuran pyran hybrid prevents glucocorticoid induced osteoporosis in mice via modulation of canonical Wnt/β-catenin signaling

Benzofuran pyran hybrid prevents glucocorticoid induced osteoporosis in mice via modulation of canonical Wnt/β-catenin signaling

Comparative Analysis of the Bioactive Compounds in Chicken Cartilage: Protective Effects of Chondroitin Sulfate and Type II Collagen Peptides Against Osteoarthritis Involve Gut Microbiota

Intra-Articular Injection of Umbilical Cord Mesenchymal Stem Cells Loaded With Graphene Oxide Granular Lubrication Ameliorates Inflammatory Responses and Osteoporosis of the Subchondral Bone in Rabbits of Modified Papain-Induced Osteoarthritis

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