Inhibition of Caco-2 and HeLa proliferation by Terminalia carpentariae C. T. White and Terminalia grandiflora Benth. extracts: Identification of triterpenoid components

Courtney, Reece; Sirdaarta, Joseph; White, Alan; Cock, Ian Edwin

doi:10.5530/pj.2017.4.74

Cited by 5 publications

(2 citation statements)

References 39 publications

(62 reference statements)

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“…Our findings are consistent with reports showing antiproliferative and/or toxic effects of polyphenols or polyphenol rich extracts in Caco-2 cells and other cell lines, such as Courtney et al [41], Sánchez-Vioque et al [42] and Szewczyk et al [43] with Terminalia carpentariae and Terminalia grandiflora extracts, Oenothera paradoxa seed extract and Crocus sativus L. leaf extract, respectively. However, it is important to note that the authors reported concentrations between 0.5 to 1 mg mL −1 to exert a more potent inhibition of Caco-2 cell proliferation, which are 4-8 times higher compared to the highest concentration (0.125 mg mL −1 ) proved in this study.…”

Section: Effect In Caco-2 Cells Viabilitysupporting

confidence: 93%

Moringa oleifera—Storage Stability, In Vitro-Simulated Digestion and Cytotoxicity Assessment of Microencapsulated Extract

et al. 2020

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Moringa extract was microencapsulated for the first time by spray-drying technique using tragacanth gum (MorTG) to improve its stability under gastrointestinal and storage conditions, assessing total polyphenolic content (TPC) and antioxidant activity. Additionally, cytotoxicity of the microencapsulated components was evaluated after contact with Caco-2 cells. Results showed that TPC was released as follows—oral (9.7%) < gastric (35.2%) < intestinal (57.6%). In addition, the antioxidant activity in in vitro digestion reached up to 16.76 ±0.15 mg GAE g−1, which was 300% higher than the initial value. Furthermore, microencapsulated moringa extract presented a half-life up to 45 days of storage, where the noticeably change was observed at 35 °C and 52.9% relative humidity. Finally, direct treatment with 0.125 mg mL−1 MorTG on Caco-2 cells showed a slight antiproliferative effect, with a cell viability of approx. 87%. Caco-2 cells’ viability demonstrated non-cytotoxicity, supporting the safety of the proposed formulation and potential use within the food field.

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Section: Effect In Caco-2 Cells Viabilitysupporting

confidence: 93%

Moringa oleifera—Storage Stability, In Vitro-Simulated Digestion and Cytotoxicity Assessment of Microencapsulated Extract

et al. 2020

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“…(T. carpentariae and T. grandiflora) were also recently reported to be good inhibitors of Caco2 and HeLa cell proliferation. 26 That study identified 3 lanostane and 2 pentacyclic triterpenoids in the extracts and postulated that they may contribute to this activity. Despite these previous studies, limited information is available on the anticancer activity of other Terminalia spp.…”

Section: Introductionmentioning

confidence: 99%

Anti-Proliferative Properties of Terminalia sericea Burch. Ex Dc Leaf Extracts Against Caco2 and HeLa Cancer Cell Lines

Gu¹,

Shalom²,

Cock³

2018

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Introduction: Terminalia spp. are characterised by their high levels of antioxidant phytochemicals and several species have anticancer activity. This study examines the anti-proliferative activity of T. sericea leaf extracts against Caco2 and HeLa carcinoma cell proliferation. Methods: Solvent extracts were prepared from T. sericea leaves and their antioxidant capacities were determined by the DPPH free radical scavenging assay. Anti-proliferative activities against Caco2 and HeLa cancer cells were determined by an MTS based cell proliferation assay. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: The methanolic and aqueous T. sericea leaf extracts displayed high antioxidant capacities (equivalent to 150 and 340 mg of ascorbic acid per gram of plant material extracted respectively). In contrast, the ethyl acetate, chloroform and hexane extracts had relatively low antioxidant contents (≤5 mg of ascorbic acid equivalents per gram of plant material extracted). The antioxidant contents of the T. sericea leaf extracts correlated with the ability of the extracts to inhibit proliferation of Caco2 and HeLa cancer cell lines. The high antioxidant methanolic and aqueous extracts were potent inhibitors of cell proliferation, with IC 50 values 120-1400 µg/mL. The aqueous T. sericea leaf extract was particularly effective, with IC 50 values of 528 and 120 µg/mL against Caco2 and HeLa cells respectively. The methanolic extract also displayed good, albeit substantially less potent, antiproliferative activity against HeLa cells, with an IC 50 of 1358 µg/mL. In contrast, the lower antioxidant content extracts generally did not inhibit cancer cell proliferation. Cell imaging studies detected morphological features consistent with apoptosis in Caco2 cells exposed to sub-lethal concentrations of the methanolic and aqueous T. sericea leaf extracts, indicating that these extracts are functioning by cytotoxic mechanisms. The aqueous T. sericea leaf extract displayed low to moderate toxicity in the Artemia franciscana bioassay, with an LC 50 value of 737 µg/mL. All other extracts were nontoxic. Conclusion: The antiproliferative activity and low toxicity of the T. sericea methanolic and aqueous leaf extracts extracts against HeLa and Caco2 cancer cell lines indicates their potential in the treatment and prevention of some cancers.

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Plants of the genus Terminalia: Phytochemical and antioxidant profiles, proliferation, and cancer

Cock

Cheesman

2021

Cancer

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Inhibition of Caco-2 and HeLa proliferation by Terminalia carpentariae C. T. White and Terminalia grandiflora Benth. extracts: Identification of triterpenoid components

Cited by 5 publications

References 39 publications

Moringa oleifera—Storage Stability, In Vitro-Simulated Digestion and Cytotoxicity Assessment of Microencapsulated Extract

Moringa oleifera—Storage Stability, In Vitro-Simulated Digestion and Cytotoxicity Assessment of Microencapsulated Extract

Anti-Proliferative Properties of Terminalia sericea Burch. Ex Dc Leaf Extracts Against Caco2 and HeLa Cancer Cell Lines

Plants of the genus Terminalia: Phytochemical and antioxidant profiles, proliferation, and cancer

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