Inhibition of Ca2+-Release–Activated Ca2+ Channel (CRAC) and K+ Channels by Curcumin in Jurkat-T Cells

Shin, Dong Hoon; Seo, Eun Yeong; Pang, Bo; Nam, Joo Hyun; Kim, Hyang Sun; Kim, Woo Kyung; Kim, Sung Joon

doi:10.1254/jphs.10209fp

Cited by 41 publications

(31 citation statements)

References 34 publications

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“…Curcumin may also induce antiinflammatory action via inhibition of Ca 2+ -releaseactivated Ca 2+ channels and K + channels in lymphocytes when it is orally administered (95). However, considering the limited pharmacokinetic availability of orally taken curcumin, careful interpretation is required for determining the effects in vivo (95). Recent studies suggest the possibility that a,b-unsaturated carbonyl based compounds might serve as the leading molecules for the design and development of improved antiinflammatory agents (96).…”

Section: Anti-inflammatory Activity In Human Gingivitis Modelmentioning

confidence: 99%

Biological Activities and Possible Dental Application of Three Major Groups of Polyphenols

Sakagami

2014

J Pharmacol Sci

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Abstract. The present article reviewed the biological activities and possible dental application of three major polyphenols, i.e., lignin-carbohydrate complexes, tannins, and flavonoids, citing mostly our in vitro studies together with those from other groups. All these polyphenols showed much lower tumor-selective cytotoxicity against oral squamous cell carcinoma cells vs. normal oral cells (gingival fibroblast, pulp cell, periodontal ligament fibroblast), in comparison to popular chemotherapeutic antitumor drugs. Several compounds showing higher tumor-selectivity did not induce internucleosomal DNA fragmentation, a biochemical hallmark of apoptosis, in oral carcinoma cell lines. Lignin-carbohydrate complex protected the cells from the cytopathic effects of HIV infection and UV irradiation more efficiently than other polyphenols. Limited digestion of lignin-carbohydrate complex suggests that the lignin moiety is involved in the prominent anti-HIV activity, whereas the carbohydrate moiety may function in immunopotentiating activity through a cell surface receptor. Alkaline extract of plant leaf, which contains higher amounts of lignincarbohydrate complex, showed potent anti-inflammatory action against IL-1b-stimulated human gingival fibroblasts. Local application of lignin-carbohydrate complex through oral mucosa is recommended, considering its poor intestinal absorption.

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Section: Anti-inflammatory Activity In Human Gingivitis Modelmentioning

confidence: 99%

Biological Activities and Possible Dental Application of Three Major Groups of Polyphenols

Sakagami

2014

J Pharmacol Sci

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“…Pretreatment with cyanidin-3-glucoside (15 µg/ml) for 30 min significantly inhibited the thapsigargin-induced SOCE responses by 57.8% and the ATP-induced responses by 88.0%. It has been reported that curcumin, a non-flavonoid polyphenol [23], inhibits SOCE in Jukat-T cells [24]. …”

Section: Resultsmentioning

confidence: 99%

Cyanidin-3-glucoside Inhibits ATP-induced Intracellular Free Ca²⁺Concentration, ROS Formation and Mitochondrial Depolarization in PC12 Cells

Perveen

Yang

et al. 2014

Korean J Physiol Pharmacol

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Flavonoids have an ability to suppress various ion channels. We determined whether one of flavonoids, cyanidin-3-glucoside, affects adenosine 5'-triphosphate (ATP)-induced calcium signaling using digital imaging methods for intracellular free Ca2+ concentration ([Ca2+]i), reactive oxygen species (ROS) and mitochondrial membrane potential in PC12 cells. Treatment with ATP (100µM) for 90 sec induced [Ca2+]i increases in PC12 cells. Pretreatment with cyanidin-3-glucoside (1µ g/ml to 100µg/ml) for 30 min inhibited the ATP-induced [Ca2+]i increases in a concentration-dependent manner (IC50=15.3µg/ml). Pretreatment with cyanidin-3-glucoside (15µg/ml) for 30 min significantly inhibited the ATP-induced [Ca2+]i responses following removal of extracellular Ca2+ or depletion of intracellular [Ca2+]i stores. Cyanidin-3-glucoside also significantly inhibited the relatively specific P2X2 receptor agonist 2-MeSATP-induced [Ca2+]i responses. Cyanidin-3-glucoside significantly inhibited the thapsigargin or ATP-induced store-operated calcium entry. Cyanidin-3-glucoside significantly inhibited the ATP-induced [Ca2+]i responses in the presence of nimodipine and ω-conotoxin. Cyanidin-3-glucoside also significantly inhibited KCl (50 mM)-induced [Ca2+]i increases. Cyanidin-3-glucoside significantly inhibited ATP-induced mitochondrial depolarization. The intracellular Ca2+ chelator BAPTA-AM or the mitochondrial Ca2+ uniporter inhibitor RU360 blocked the ATP-induced mitochondrial depolarization in the presence of cyanidin-3-glucoside. Cyanidin-3-glucoside blocked ATP-induced formation of ROS. BAPTA-AM further decreased the formation of ROS in the presence of cyanidin-3-glucoside. All these results suggest that cyanidin-3-glucoside inhibits ATP-induced calcium signaling in PC12 cells by inhibiting multiple pathways which are the influx of extracellular Ca2+ through the nimodipine and ω-conotoxin-sensitive and -insensitive pathways and the release of Ca2+ from intracellular stores. In addition, cyanidin-3-glucoside inhibits ATP-induced formation of ROS by inhibiting Ca2+-induced mitochondrial depolarization.

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“…Interestingly, when stimulated by ConA, a sluggish increase in [Ca 2+ ] c was observed in the presence of Gd 3+ , and the Gd 3+ -resistant Ca 2+ influx was inhibited by 30 μM 2-APB. αCD3-induced Δ[Ca 2+ ] c was previously shown to be completely inhibited by 2-APB at above 30 μM [22,29]. The summaries of [Ca 2+ ] c at initial peak and at 400 s from the agonist application are shown as bar graphs.…”

Section: Resultsmentioning

confidence: 99%

Differential pathways for calcium influx activated by concanavalin A and CD3 stimulation in Jurkat T cells

Pang

Shin

Park

et al. 2011

Pflugers Arch - Eur J Physiol

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Sustained increase in [Ca(2+)](c) (Δ[Ca(2+)](c)) is a critical early signal from T-cell receptor (TCR/CD3). In general, Ca(2+)-release activated Ca(2+) channels (CRAC) are responsible for the Ca(2+) influx and Δ[Ca(2+)](c) after TCR/CD3 stimulation. However, T cells also express Ca(2+)-permeable nonselective cation channels such as TRPM2 and TRPC. Gd(3+) is a relatively selective blocker for CRAC at micromolar concentrations. Here, Jurkat T cells were used to investigate the Gd(3+)-resistant Ca(2+) influx (Δ[Ca(2+)](c,Gd)) induced by concanavalin A (ConA, 1 μg/ml), a widely used mitogenic agent for T cells, or by anti-CD3 Ab (αCD3). αCD3-induced Δ[Ca(2+)](c) was partly (~60%) inhibited by 1 μM Gd(3+) while thapsigargin-induced Δ[Ca(2+)] was almost completely abolished. ConA-induced Δ[Ca(2+)] was mostly inhibited by 1 μM Gd(3+) during the early phase (<30 s of ConA application) and became resistant during the late phase (>2 min). Induction of Δ[Ca(2+)](c,Gd) by αCD3 and ConA was inhibited by 2-aminoethoxydiphenyl borate (2-APB) and by N-(p-amylcinnamoyl) anthranilic acid, indicating that TRPM2 and TRPC are involved in this process. Treatment with Pyr-3, a TRPC3-specific inhibitor, potently suppressed Δ[Ca(2+)](c,Gd) by αCD3 (IC(50), 0.16 μM). Patch clamp experiments demonstrated that the TRPM2 channels were activated by ConA, and the TRPC-like channels were activated by αCD3. Our present study suggests that TRPM2 and TRPC3 are activated by ConA and TCR/CD3, respectively, in Jurkat T cells and are responsible for the induction of Δ[Ca(2+)](c,Gd).

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Inhibition of Ca2+-Release–Activated Ca2+ Channel (CRAC) and K+ Channels by Curcumin in Jurkat-T Cells

Cited by 41 publications

References 34 publications

Biological Activities and Possible Dental Application of Three Major Groups of Polyphenols

Biological Activities and Possible Dental Application of Three Major Groups of Polyphenols

Cyanidin-3-glucoside Inhibits ATP-induced Intracellular Free Ca²⁺Concentration, ROS Formation and Mitochondrial Depolarization in PC12 Cells

Differential pathways for calcium influx activated by concanavalin A and CD3 stimulation in Jurkat T cells

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Inhibition of Ca2+-Release–Activated Ca2+ Channel (CRAC) and K+ Channels by Curcumin in Jurkat-T Cells

Cited by 41 publications

References 34 publications

Biological Activities and Possible Dental Application of Three Major Groups of Polyphenols

Biological Activities and Possible Dental Application of Three Major Groups of Polyphenols

Cyanidin-3-glucoside Inhibits ATP-induced Intracellular Free Ca2+Concentration, ROS Formation and Mitochondrial Depolarization in PC12 Cells

Differential pathways for calcium influx activated by concanavalin A and CD3 stimulation in Jurkat T cells

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Cyanidin-3-glucoside Inhibits ATP-induced Intracellular Free Ca²⁺Concentration, ROS Formation and Mitochondrial Depolarization in PC12 Cells