Inhibition of biofilms of <i>Pseudomonas aeruginosa by</i> Medihoney <i>in vitro</i>

Cooper, Rose; Jenkins, Leighton; Hooper, Samuel James

doi:10.12968/jowc.2014.23.3.93

Cited by 49 publications

(47 citation statements)

References 29 publications

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“…Honey has been used successfully in wound care, and its potential antibiofilm properties have recently been indicated, particularly against Staphylococcus aureus and P. aeruginosa (Cooper et al, 2014;Lu et al, 2014). However, little information is available concerning the potential antibiofilm components of honey.…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the antibiofilm activity of honey defensin-1 (Def-1) in its recombinant form. A modified Lubbock chronic wound biofilm formed by four bacterial species (Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Enterococcus faecalis) was used for evaluation of honey and recombinant bee-derived Def-1 antibiofilm efficacy. Recombinant Def-1 was prepared by heterologous expression in Escherichia coli. We showed that different types of honey (manuka and honeydew) were able to significantly reduce the cell viability of wound pathogens (Staphylococcus aureus, Streptococcus agalactiae and Pseudomonas aeruginosa) in mature polymicrobial biofilm. None of the tested honeys showed the ability to eradicate Enterococcus faecalis in biofilm. In addition, recombinant Def-1 successfully reduced the viability of Staphylococcus aureus and Pseudomonas aeruginosa cells within established polymicrobial biofilm after 24 and 48 h of treatment. Interestingly, recombinant Def-1 did not affect the viability of Streptococcus agalactiae cells within the biofilm, whereas both natural honeys significantly reduced the viable bacteria. Although Enterococcus faecalis was highly resistant to Def-1, Def-1 significantly affected the biofilm formation of Enterococcus faecalis and Streptococcus agalactiae after 24 h of treatment, most likely by inhibiting its extracellular polymeric substances production. In conclusion, our study revealed that honey and Def-1 are effective against established multispecies biofilm; however, Enterococcus faecalis grown in multispecies biofilm was resistant to both antimicrobials.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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“…The monitoring of biofilm formation by the strain CECT 8546 was successfully visualised by epifluorescence microscopy using the Live/ Dead BacLight Kit. These dyes have been applied to investigate the development of several biofilms (Cooper et al 2014;Neu and Lawrence 1997), but, to the best of our knowledge, have never been used to monitor AAB biofilms. The strain CECT 8546 produced cellulose very quickly; large amounts of cellulose floating in the medium were detected after a few hours of growth.…”

Section: Discussionmentioning

confidence: 99%

“…Several images of this structure were obtained by scanning electron microscopy (Gomes et al 2013;Mikkelsen et al 2009;Trovatti et al 2011). However, while the development of several biofilms has been studied using fluorescent dyes like SYTO 9 or live/ dead stains (Cooper et al 2014;Neu and Lawrence 1997), to our knowledge, these methods have not been applied specifically to study AAB biofilms. The molecular structure of BC allows the use of specific dyes like Congo red or Calcofluor to screen for cellulose-producing strains (Römling 2002;Zogaj et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Cellulose production and cellulose synthase gene detection in acetic acid bacteria

Valera

Torija

Mas

et al. 2014

Appl Microbiol Biotechnol

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The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration by the traditional method. The vinegar biofilm produced by AAB on the air-liquid interface is primarily composed of cellulose and maintains the cells in close contact with oxygen. In this study, we screened for the ability of AAB to produce cellulose using different carbon sources in the presence or absence of ethanol. The presence of cellulose in biofilms was confirmed using the fluorochrome Calcofluor by microscopy. Moreover, the process of biofilm formation was monitored under epifluorescence microscopy using the Live/Dead BacLight Kit. A total of 77 AAB strains belonging to 35 species of Acetobacter, Komagataeibacter, Gluconacetobacter, and Gluconobacter were analysed, and 30 strains were able to produce a cellulose biofilm in at least one condition. This cellulose production was correlated with the PCR amplification of the bcsA gene that encodes cellulose synthase. A total of eight degenerated primers were designed, resulting in one primer pair that was able to detect the presence of this gene in 27 AAB strains, 26 of which formed cellulose.

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“…No loss of bacterial sensibility to honey and no appearance of bacteria resistant mutants were evidenced in time [17]. Moreover, bacteria aggregated in biofilms are sensitive to honey [3,[18][19][20][21] and resistant to antibiotics and silver dressings [22]. It might explain why honey succeeded in many cases, where other antibacterial therapies failed [23,24].…”

Section: The Anti-infectious Activity Of Honeymentioning

confidence: 99%

Honey, Food and Medicine: Scientific Rationale and Practical Efficiency in External Administration of Medicinal Honey for Wound Healing

Zbuchea¹

2017

JAST-B

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Except for its benefits as food and medicine, honey has been used since ancient times as an external remedy in wound care, and has been the subject of numerous studies and research, that reveal its complex favorable action for wound healing. Based on scientific research and clinical studies published in literature, this work presents these various properties and therapeutic activities of honey dressings. Next, actions and clinical effects of L-Mesitran range of dressings, based on medicinal honey and authorized European Union (EU) and Food and Drug Administration (FDA), have been highlighted in a case series of patients in the Department of Plastic Surgery, Emergency District Hospital of Ploiesti, Romania, by monitoring the clinical local evolution of burns and other wounds. L-Mesitran range of products is based on the combination of medicinal honey and other ingredients, such as hypoallergenic medicinal lanolin, vitamins C and E. These sterilized preparations were applied for various skin lesions after wound cleansing, and exerted a favorable action on healing of burns and other wounds, sometimes in combination with surgical treatment, being applied in these cases before or after surgery. Further clinical studies are needed to establish the best indications, methods and forms of administration for each type of wounds, as well as the selection over other types of dressings.

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Inhibition of biofilms of Pseudomonas aeruginosa by Medihoney in vitro

Abstract: This study was sponsored by Derma Sciences Inc, NJ. An unrestricted grant was provided and the sponsors were not involved in the design of the experiments or the preparation of this manuscript.

Cited by 49 publications

References 29 publications

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Cellulose production and cellulose synthase gene detection in acetic acid bacteria

Honey, Food and Medicine: Scientific Rationale and Practical Efficiency in External Administration of Medicinal Honey for Wound Healing

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