2014
DOI: 10.1038/ja.2014.143
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Inhibition of biofilm formation in Bacillus subtilis by new halogenated furanones

Abstract: Gram-positive bacteria can cause various infections including hospital-acquired infections. While in the biofilm, the resistance of bacteria to both antibiotics and the human immune system is increased causing difficulties in the treatment. Bacillus subtilis, a non-pathogenic Gram-positive bacterium, is widely used as a model organism for studying biofilm formation. Here we investigated the effect of novel synthesized chloro- and bromo-containing 2(5H)-furanones on biofilm formation by B. subtilis. Mucobromic … Show more

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Cited by 55 publications
(62 citation statements)
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“…We investigated whether Ficin is able to disrupt bacterial biofilms formed by S. aureus and S. epidermidis , the bacteria colonizing wounds and thus retarding wound healing30. To do so, the bacteria were grown in BM broth earlier developed3132 for 72 h on 24-well TC-treated plates that provided a representative and repeatable formation of the rigid biofilm strongly attached to the surfaces, in contrast to Müller-Hinton broth, Trypticase soy broth or LB-medium (Fig. 1).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…We investigated whether Ficin is able to disrupt bacterial biofilms formed by S. aureus and S. epidermidis , the bacteria colonizing wounds and thus retarding wound healing30. To do so, the bacteria were grown in BM broth earlier developed3132 for 72 h on 24-well TC-treated plates that provided a representative and repeatable formation of the rigid biofilm strongly attached to the surfaces, in contrast to Müller-Hinton broth, Trypticase soy broth or LB-medium (Fig. 1).…”
Section: Resultsmentioning
confidence: 99%
“…The Müller-Hinton broth (Fluka) or Trypticase soy broth (Sigma) did not provide stable biofilm formation by both Staphylococcus aureus and Staphylococcus epidermidis , as is has been determined in preliminary studies (Fig. 1), thus the modified Basal medium (BM) (glucose 5 g, peptone 7 g, MgSO 4  × 7H 2 O 2.0 g and CaCl 2  × 2H 2 O 0.05 g in 1.0 liter tap water) was used for the biofilm formation assays3139. Bacteria were grown for 48–72 hours as indicated under static conditions at 37 °C to obtain rigid biofilm structures.…”
Section: Methodsmentioning
confidence: 99%
“…The product was purified by flash column chromatography. The furanone carbonyl does not appear on the 13 C NMR spectrum, this can be rationalized due to the slow relaxation basis.…”
Section: Synthetic Chemistrymentioning
confidence: 99%
“…Column chromatography was performed using Merck Kielselgel 60 (particle size 0.040-0.063 mm), while thin layer chromatography was done on Merck aluminum-supported silica gel 60 F 254 . NMR spectra were recorded at 400 MHz for 1 H NMR and 100 MHz for 13 C NMR on a Varian Unity INOVA 400 NMR as a solution in either CDCl 3 or DMSO-d 6 . Chemical shift data (d-values) are recorded in parts per million (ppm) relative to tetramethylsilane as an internal standard, coupling constants are quoted in hertz (see Supporting Information file for representative NMR spectra).…”
Section: Synthetic Chemistrymentioning
confidence: 99%
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