Inhibition of binding of<i>Helicobacter pylori</i>to the glycolipid receptors by probiotic<i>Lactobacillus reuteri</i>

Mukai, T.; Asasaka, Tomoko; Sato, Eri; Mori, Kenichi; Matsumoto, Mitsuyo; Ohori, Hitoshi

doi:10.1111/j.1574-695x.2002.tb00541.x

Cited by 221 publications

(50 citation statements)

References 32 publications

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“…The production of antimicrobial substances, such as lactic and acetic acid, is one example of this type of environmental modification [98]. Some lactobacilli and bifidobacteria share carbohydrate-binding specificities with some enteropathogens [99,100], which makes it possible for the strains to compete with specific pathogens for the receptor sites on host cells [101]. In general, probiotic strains are able to inhibit the attachment of pathogenic bacteria by means of steric hindrance at enterocyte pathogen receptors [102].…”

Section: Mechanisms Of Action Of Probioticsmentioning

confidence: 99%

Probiotic Mechanisms of Action

et al. 2012

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Probiotics are live microorganisms that provide health benefits to the host when ingested in adequate amounts. The strains most frequently used as probiotics include lactic acid bacteria and bifidobacteria. Probiotics have demonstrated significant potential as therapeutic options for a variety of diseases, but the mechanisms responsible for these effects have not been fully elucidated yet. Several important mechanisms underlying the antagonistic effects of probiotics on various microorganisms include the following: modification of the gut microbiota, competitive adherence to the mucosa and epithelium, strengthening of the gut epithelial barrier and modulation of the immune system to convey an advantage to the host. Accumulating evidence demonstrates that probiotics communicate with the host by pattern recognition receptors, such as toll-like receptors and nucleotide-binding oligomerization domain-containing protein-like receptors, which modulate key signaling pathways, such as nuclear factor-ĸB and mitogen-activated protein kinase, to enhance or suppress activation and influence downstream pathways. This recognition is crucial for eliciting measured antimicrobial responses with minimal inflammatory tissue damage. A clear understanding of these mechanisms will allow for appropriate probiotic strain selection for specific applications and may uncover novel probiotic functions. The goal of this systematic review was to explore probiotic modes of action focusing on how gut microbes influence the host.

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Section: Mechanisms Of Action Of Probioticsmentioning

confidence: 99%

Probiotic Mechanisms of Action

et al. 2012

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“…Lactobacilli and bifidobacteria have been shown to inhibit a broad range of pathogens including E. coli , Salmonella , Helicobacter pylori , Listeria monocytogenes , and Rotavirus [21,51,52,53,54,55,56,57]. It has been shown that some lactobacilli and bifidobacteria compete for binding to host cell receptors because they share the same carbohydrate-binding specificities with some enteropathogens [58,59,60]. Lactobacillus rhamnosus has been shown to inhibit the internalization of enterohemorrhagic E. coli (EHEC) [61].…”

Section: Discussionmentioning

confidence: 99%

Probiotic Microorganisms Inhibit Epithelial Cell Internalization of Botulinum Neurotoxin Serotype A

Lam

Tam

Stanker

et al. 2016

Toxins

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Botulinum neurotoxins (BoNTs) are some of the most poisonous natural toxins known to man and are threats to public health and safety. Previous work from our laboratory showed that both BoNT serotype A complex and holotoxin can bind and transit through the intestinal epithelia to disseminate in the blood. The timing of BoNT/A toxin internalization was shown to be comparable in both the Caco-2 in vitro cell culture and in the oral mouse intoxication models. Probiotic microorganisms have been extensively studied for their beneficial effects in not only maintaining the normal gut mucosa but also protection from allergens, pathogens, and toxins. In this study, we evaluate whether probiotic microorganisms will block BoNT/A uptake in the in vitro cell culture system using Caco-2 cells. Several probiotics tested (Saccharomyces boulardii, Lactobacillus acidophilus, Lactobacillus rhamnosus LGG, and Lactobacillus reuteri) blocked BoNT/A uptake in a dose-dependent manner whereas a non-probiotic strain of Escherichia coli did not. We also showed that inhibition of BoNT/A uptake was not due to the degradation of BoNT/A nor by sequestration of toxin via binding to probiotics. These results show for the first time that probiotic treatment can inhibit BoNT/A binding and internalization in vitro and may lead to the development of new therapies.

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“…For example, treatment of broiler chicken with a multi-species probiotic consisting of bacteria isolated from the chicken gut prevented their colonization by Campylobacter jejuni 121 . The beneficial effects of probiotics are, to some extent, due to competitive exclusion of pathogenic bacteria from host binding sites, 122 - 124 although this is challenging to demonstrate in vivo because of the complexity of the probiotics’ mechanisms of action. Over recent years, probiotics have been specifically engineered to mimic sugars on host receptors, thereby blocking the host cell binding of toxins released by pathogenic bacteria including ETEC, shiga toxin-producing E. coli (STEC) and V. cholerae 125 - 127 .…”

Section: Improving Efficacy By Exploiting Multivalencymentioning

confidence: 99%

Targeting the bacteria–host interface

2013

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Bacterial infections are a major cause of morbidity and mortality worldwide and are increasingly problematic to treat due to the rise in antibiotic-resistant strains. It becomes more and more challenging to develop new antimicrobials that are able to withstand the ever-increasing repertoire of bacterial resistance mechanisms. This necessitates the development of alternative approaches to prevent and treat bacterial infections. One of the first steps during bacterial infection is adhesion of the pathogen to host cells. A pathogen’s ability to colonize and invade host tissues strictly depends on this process. Thus, interference with adhesion (anti-adhesion therapy) is an efficient way to prevent or treat bacterial infections. As a basis to present different strategies to interfere with pathogen adhesion, this review briefly introduces general concepts of bacterial attachment to host cells. We further discuss advantages and disadvantages of anti-adhesion treatments and issues that are in need of improvement so as to make anti-adhesion compounds a more broadly applicable alternative to conventional antimicrobials.

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Inhibition of binding ofHelicobacter pylorito the glycolipid receptors by probioticLactobacillus reuteri

Cited by 221 publications

References 32 publications

Probiotic Mechanisms of Action

Probiotic Mechanisms of Action

Probiotic Microorganisms Inhibit Epithelial Cell Internalization of Botulinum Neurotoxin Serotype A

Targeting the bacteria–host interface

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