Inhibition of basophil activation by histamine: a sensitive and reproducible model for the study of the biological activity of high dilutions

Sainte-Laudy, J.; Belon, Philippe

doi:10.1016/j.homp.2009.09.009

Cited by 34 publications

(30 citation statements)

References 55 publications

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“…Indeed, some groups and commercial kits use EDTA as an anti-coagulant and add calcium/magnesium to enable the stimulus-dependent induction of a CD63 hi basophil population. 58,59 However, adding exogenous calcium/magnesium lengthens the procedure and could cause variable results. Moreover, we found that calcium/magnesium affected not only CD63 but also CD203c expression levels, including baseline levels (Fig 3, C and D ).…”

Section: Discussionmentioning

confidence: 99%

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis

Mukai

Gaudenzio

Gupta

et al. 2017

Journal of Allergy and Clinical Immunology

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Background Basophil activation tests (BATs) have promise for research and for clinical monitoring of subjects with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies. Objective To attempt to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample. Methods Blood from 46 healthy donors and 120 peanut allergic patients was collected into ethylenediaminetetraacetic acid (EDTA) or heparin tubes, and samples were stored at 4°C or room temperature for 4 or 24 hours before performing BATs. Results Stimulation with anti-Immunoglobulin E (anti-IgE) or interleukin-3 (IL-3) resulted in strong upregulation of basophil CD203c in samples collected in EDTA or heparin, stored at 4°C, and analyzed 24 hours after sample collection. However, a CD63hi population of basophils was not observed in any conditions in EDTA-treated samples unless exogenous calcium/magnesium was added at the time of anti-IgE stimulation. By contrast, blood samples collected in heparin tubes were adequate for quantification of upregulation of basophil CD203c and for identification of a population of CD63hi basophils, whether the specimens were analyzed by conventional flow cytometry or by Cytometry by Time-of-Flight mass spectrometry (CyTOF), and such tests could be performed after blood was stored for 24 hours at 4°C. Conclusion BATs to measure upregulation of basophil CD203c and induction of a CD63hi basophil population can be conducted using blood obtained in heparin tubes and stored at 4°C for 24 hours.

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Section: Discussionmentioning

confidence: 99%

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis

Mukai

Gaudenzio

Gupta

et al. 2017

Journal of Allergy and Clinical Immunology

View full text Add to dashboard Cite

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“…This line of research clearly demonstrates the biphasic action of a substance: a small dose can exert a stimulatory effect on the recovery and the development of survival capacity of cells that have been previously disturbed by a high dose of the same substance. It is of interest that this stimulatory effect of low dose stress is dependent on the initial exposure condition: the more severe the initial stress conditions, the smaller the 146,172 Bacterial peptides Stimulate adherence Low doses inhibit adherence Leukocytes 173 Podophyllotoxin Cell toxicity Low doses enhance oxidative metabolism Wheat germ 174e176 Arsenite Cell toxicity HDs protect from toxicity Lymphocytes 177,178 Cadmium Cell toxicity HDs protect from cadmium toxicity Neurons 179,180 Glutamate, cycloheximide Neurotoxicity Extremely low doses and HDs protect from neurotoxicity Basophils 14 Histamine Inflammation HDs inhibit basophil activation Basophils 181 Quercetin Inhibit cell activation Low doses enhance formyl-peptide-induced activation concentration required for stimulating survival and hsps induction.…”

Section: Hormesismentioning

confidence: 99%

High-dilution effects revisited. 2. Pharmacodynamic mechanisms

et al. 2014

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The pharmacodynamics aspects of homeopathic remedies are appraised by laboratory studies on the biological effects at various levels (cellular, molecular and systemic). The major question is how these medicines may work in the body. The possible answers concern the identification of biological targets, the means of drug-receptor interactions, the mechanisms of signal transmission and amplification, and the models of inversion of effects according to the traditional 'simile' rule. These problems are handled by two experimental and theoretical lines, according to the doses or dilutions considered (low-medium versus high dilutions). Homeopathic formulations in low-medium dilutions, containing molecules in the range of ultra-low doses, exploit the extreme sensitivity of biological systems to exogenous and endogenous signals. Their effects are interpreted in the framework of hormesis theories and paradoxical pharmacology. The hypotheses regarding the action mechanisms of highly diluted/dynamized solutions (beyond Avogadro-Loschmidt limit) variously invoke sensitivity to bioelectromagnetic information, participation of water chains in signalling, and regulation of bifurcation points of systemic networks. High-dilution pharmacology is emerging as a pioneering subject in the domain of nanomedicine and is providing greater plausibility to the puzzling claims of homeopathy.

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“…Dilutions such as the 4th and 5th centesimal (4C–5C) dilutions from crude extracts are expected to contain ultra-low concentrations of the purported active ingredient, but still falling within the molecular range since the dilution factor from the first ethanolic extract (mother tincture) is 10 8 –10 10 times. On the other hand, dilutions of 12C (10 24 times) or higher theoretically contain no molecules of the mother tincture since the dilution factor exceeds the Avogadro–Loschmidt limit (Sainte-Laudy and Belon 2009). …”

Section: Introductionmentioning

confidence: 99%

Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

et al. 2010

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IntroductionThis study was designed to investigate the putative anxiolytic-like activity of ultra-low doses of Gelsemium sempervirens (G. sempervirens), produced according to the homeopathic pharmacopeia.MethodsFive different centesimal (C) dilutions of G. sempervirens (4C, 5C, 7C, 9C and 30C), the drug buspirone (5 mg/kg) and solvent vehicle were delivered intraperitoneally to groups of ICR-CD1 mice over a period of 9 days. The behavioral effects were assessed in the open-field (OF) and light–dark (LD) tests in blind and randomized fashion.ResultsMost G. sempervirens dilutions did not affect the total distance traveled in the OF (only the 5C had an almost significant stimulatory effect on this parameter), indicating that the medicine caused no sedation effects or unspecific changes in locomotor activity. In the same test, buspirone induced a slight but statistically significant decrease in locomotion. G. sempervirens showed little stimulatory activity on the time spent and distance traveled in the central zone of the OF, but this effect was not statistically significant. In the LD test, G. sempervirens increased the % time spent in the light compartment, an indicator of anxiolytic-like activity, with a statistically significant effect using the 5C, 9C and 30C dilutions. These effects were comparable to those of buspirone. The number of transitions between the compartments of the LD test markedly increased with G. sempervirens 5C, 9C and 30C dilutions.ConclusionThe overall pattern of results provides evidence that G. sempervirens acts on the emotional reactivity of mice, and that its anxiolytic-like effects are apparent, with a non-linear relationship, even at high dilutions.

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Inhibition of basophil activation by histamine: a sensitive and reproducible model for the study of the biological activity of high dilutions

Cited by 34 publications

References 55 publications

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis

High-dilution effects revisited. 2. Pharmacodynamic mechanisms

Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

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