Inhibition of bacterial motility and spreading via release of cranberry derived materials from silicone substrates

Chan, Michelle; Hidalgo, Gabriela; Asadishad, Bahareh; Almeida, S.; Muja, Naser; Mohammadi, Maziar Shah; Nazhat, Showan N.; Tufenkji, Nathalie

doi:10.1016/j.colsurfb.2013.03.047

Cited by 13 publications

(17 citation statements)

References 45 publications

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“…Silicone discs (1-cm diameter) were prepared as described previously (25), placed into wells of a 24-well plate, and incubated overnight at 37°C with 0.5 ml human plasma (P9523; Sigma-Aldrich Canada). After incubation, the plasma solution was removed and replaced with 1 ml of ϳ10 6 CFU ml Ϫ1 inoculum of the challenge organism in MHB-II (Oxoid; Fisher Scientific Canada).…”

Section: Methodsmentioning

confidence: 99%

Polyphenolic Extract from Maple Syrup Potentiates Antibiotic Susceptibility and Reduces Biofilm Formation of Pathogenic Bacteria

Maisuria

Hosseinidoust

Tufenkji

2015

Appl Environ Microbiol

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Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple syrup extract (PRMSE). PRMSE exhibited antimicrobial activity as well as strong synergistic interaction with selected antibiotics against Gram-negative clinical strains of Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa. Among the phenolic constituents of PRMSE, catechol exhibited strong synergy with antibiotics as well as with other phenolic components of PRMSE against bacterial growth. At sublethal concentrations, PRMSE and catechol efficiently reduced biofilm formation and increased the susceptibility of bacterial biofilms to antibiotics. In an effort to elucidate the mechanism for the observed synergy with antibiotics, PRMSE was found to increase outer membrane permeability of all bacterial strains and effectively inhibit efflux pump activity. Furthermore, transcriptome analysis revealed that PRMSE significantly repressed multiple-drug resistance genes as well as genes associated with motility, adhesion, biofilm formation, and virulence. Overall, this study provides a proof of concept and starting point for investigating the molecular mechanism of the reported increase in bacterial antibiotic susceptibility in the presence of PRMSE. Gram-negative and Gram-positive bacteria colonize surfaces in health care settings, on indwelling medical devices, and even on live tissue, leading to infections that often are treated with antibiotic therapy. However, two major factors complicate the effectiveness of antibiotic treatments, namely, (i) the rising number of antibiotic-resistant bacteria and (ii) the formation of biofilms. These complications lead to increased patient morbidity, increased costs of treatment, and higher rates of hospitalization (1, 2). Antibiotic resistance is the inevitable evolutionary survival mechanism of bacteria, and it is aggravated by the overuse of antibiotics in the medical and farming industries. Bacterial biofilms are structured, surface-associated microbial communities, protected by a self-produced matrix of extracellular polymeric substances, and are the most common mode of bacterial growth. Formation of biofilms complicates the treatment of infections, because bacteria in biofilm mode generally are very persistent, requiring considerably higher doses of antibiotics for treatment than planktonic bacteria (3). High antibiotic doses disturb the body's microbiome, putting the patient's health at risk, as well as increasing the potential for development of antibiotic-resistant strains (4). The reduced effectiveness of current therapies and a declining repertoire of clinically useful drugs motivate research for the identification of novel molecules endowed with antimicrobial and/or antibiofilm properties.Many plants synthesize aromatic substances, most of which ar...

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Section: Methodsmentioning

confidence: 99%

Polyphenolic Extract from Maple Syrup Potentiates Antibiotic Susceptibility and Reduces Biofilm Formation of Pathogenic Bacteria

Maisuria

Hosseinidoust

Tufenkji

2015

Appl Environ Microbiol

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“…Our findings show that the higher ratio between proanthocyanidins and flavonoids in P. moniliformis fractions results in better antibiofilm activity ( Supplementary Data and Discussion 2 ) and B-type linked proanthocyanidins are the compounds responsible for the inhibition of biofilm formation by the Gram-positive bacteria. Previously, antiadherence and antibiofilm properties against Gram-negative Escherichia coli were assigned to proanthocyanidins derived from cranberry ( Vaccinium macrocarpon ) 28 29 30 31 32 33 , and their preventive effect in urinary tract infections has been clinically demonstrated 34 35 . Cranberry proanthocyanidins consist predominantly of procyanidin units 28 and the oligomers containing at least one A-type interflavan linkage account for more than 91% of the oligomers 29 .…”

Section: Discussionmentioning

confidence: 99%

Natural Green Coating Inhibits Adhesion of Clinically Important Bacteria

Trentin

Silva

Frasson

et al. 2015

Sci Rep

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Despite many advances, biomaterial-associated infections continue to be a major clinical problem. In order to minimize bacterial adhesion, material surface modifications are currently being investigated and natural products possess large potential for the design of innovative surface coatings. We report the bioguided phytochemical investigation of Pityrocarpa moniliformis and the characterization of tannins by mass spectrometry. It was demonstrated that B-type linked proanthocyanidins-coated surfaces, here termed Green coatings, reduced Gram-positive bacterial adhesion and supported mammalian cell spreading. The proposed mechanism of bacterial attachment inhibition is based on electrostatic repulsion, high hydrophilicity and the steric hindrance provided by the coating that blocks bacterium-substratum interactions. This work shows the applicability of a prototype Green-coated surface that aims to promote necessary mammalian tissue compatibility, while reducing bacterial colonization.

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“…Here, we report that a purified cranberry proanthocyanidin (cPAC) fraction potentiates the activity of a broad range of antibiotic classes against the opportunistic pathogens Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa. [6,7] cPAC are condensed tannins that can hinder bacterial attachment to cellular or biomaterial surfaces, [8][9][10][11] impair bacterial motility, [12][13][14][15][16][17] induce a state of iron limitation, [18] and interfere with quorum sensing. Remarkably, when combined with tetracycline, cPAC was able to completely prevent the evolution of resistance in E. coli and P. aeruginosa.…”

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confidence: 99%

“…[6,7] cPAC are condensed tannins that can hinder bacterial attachment to cellular or biomaterial surfaces, [8][9][10][11] impair bacterial motility, [12][13][14][15][16][17] induce a state of iron limitation, [18] and interfere with quorum sensing. [6,7] cPAC are condensed tannins that can hinder bacterial attachment to cellular or biomaterial surfaces, [8][9][10][11] impair bacterial motility, [12][13][14][15][16][17] induce a state of iron limitation, [18] and interfere with quorum sensing.…”

mentioning

confidence: 99%

Proanthocyanidin Interferes with Intrinsic Antibiotic Resistance Mechanisms of Gram‐Negative Bacteria

et al. 2019

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Antibiotic resistance is spreading at an alarming rate among pathogenic bacteria in both medicine and agriculture. Interfering with the intrinsic resistance mechanisms displayed by pathogenic bacteria has the potential to make antibiotics more effective and decrease the spread of acquired antibiotic resistance. Here, it is demonstrated that cranberry proanthocyanidin (cPAC) prevents the evolution of resistance to tetracycline in Escherichia coli and Pseudomonas aeruginosa , rescues antibiotic efficacy against antibiotic‐exposed cells, and represses biofilm formation. It is shown that cPAC has a potentiating effect, both in vitro and in vivo, on a broad range of antibiotic classes against pathogenic E. coli , Proteus mirabilis , and P. aeruginosa . Evidence that cPAC acts by repressing two antibiotic resistance mechanisms, selective membrane permeability and multidrug efflux pumps, is presented. Failure of cPAC to potentiate antibiotics against efflux pump‐defective mutants demonstrates that efflux interference is essential for potentiation. The use of cPAC to potentiate antibiotics and mitigate the development of resistance could improve treatment outcomes and help combat the growing threat of antibiotic resistance.

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Inhibition of bacterial motility and spreading via release of cranberry derived materials from silicone substrates

Cited by 13 publications

References 45 publications

Polyphenolic Extract from Maple Syrup Potentiates Antibiotic Susceptibility and Reduces Biofilm Formation of Pathogenic Bacteria

Polyphenolic Extract from Maple Syrup Potentiates Antibiotic Susceptibility and Reduces Biofilm Formation of Pathogenic Bacteria

Natural Green Coating Inhibits Adhesion of Clinically Important Bacteria

Proanthocyanidin Interferes with Intrinsic Antibiotic Resistance Mechanisms of Gram‐Negative Bacteria

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