Inhibition of bacterial biofilms by the snake venom proteome

Khan, Neyaz A.; Amorim, Fernanda Gobbi; Dunbar, John P.; Leonard, Dayle; Redureau, Damien; Quinton, Loïc; Dugon, Michel M.; Boyd, Aoife

doi:10.1016/j.btre.2023.e00810

Cited by 3 publications

(2 citation statements)

References 70 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This analysis indicates they constitute nearly 4% of all venom proteins in the KwaZulu-Natal venom and around 0.6–0.7% in two others. There are also other reports documenting the identification of Ig-like domain-containing proteins [ 37 – 38 ]. However, their presence in the venom was not discussed, likely because they do not belong to recognized toxin groups/families.…”

Section: Discussionmentioning

confidence: 99%

Venom diversity in Naja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences

Hus,

Buczkowicz,

Pietrowska

et al. 2024

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Background Intraspecific variations in snake venom composition have been extensively documented, contributing to the diverse clinical effects observed in envenomed patients. Understanding these variations is essential for developing effective snakebite management strategies and targeted antivenom therapies. We aimed to comprehensively investigate venoms from three distinct populations of N. mossambica from Eswatini, Limpopo, and KwaZulu-Natal regions in Africa in terms of their protein composition and reactivity with three commercial antivenoms (SAIMR polyvalent, EchiTAb+ICP, and Antivipmyn Africa). Methodology/Principal findings Naja mossambica venoms from Eswatini region exhibited the highest content of neurotoxic proteins, constituting 20.70% of all venom proteins, compared to Limpopo (13.91%) and KwaZulu-Natal (12.80%), and was characterized by the highest diversity of neurotoxic proteins, including neurotoxic 3FTxs, Kunitz-type inhibitors, vespryns, and mamba intestinal toxin 1. KwaZulu-Natal population exhibited considerably lower cytotoxic 3FTx, higher PLA2 content, and significant diversity in low-abundant proteins. Conversely, Limpopo venoms demonstrated the least diversity as demonstrated by electrophoretic and mass spectrometry analyses. Immunochemical assessments unveiled differences in venom-antivenom reactivity, particularly concerning low-abundance proteins. EchiTAb+ICP antivenom demonstrated superior reactivity in serial dilution ELISA assays compared to SAIMR polyvalent. Conclusions/Significance Our findings reveal a substantial presence of neurotoxic proteins in N. mossambica venoms, challenging previous understandings of their composition. Additionally, the detection of numerous peptides aligning to uncharacterized proteins or proteins with unknown functions underscores a critical issue with existing venom protein databases, emphasizing the substantial gaps in our knowledge of snake venom protein components. This underscores the need for enhanced research in this domain. Moreover, our in vitro immunological assays suggest EchiTAb+ICP’s potential as an alternative to SAIMR antivenom, requiring confirmation through prospective in vivo neutralization studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Venom diversity in Naja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences

Hus,

Buczkowicz,

Pietrowska

et al. 2024

PLoS Negl Trop Dis

View full text Add to dashboard Cite

show abstract

“…This analysis indicates they constitute nearly 4% of all venom proteins in the KwaZulu-Natal venom and around 0.6-0.7% in two others. There are also other reports documenting the identification of Ig-like domain-containing proteins [31][32]. However, their presence in the venom was not discussed, likely because they do not belong to recognized toxin groups/families.…”

Section: Discussionmentioning

confidence: 99%

Venom diversity inNaja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences

Hus,

Buczkowicz,

Pietrowska

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Intraspecific variations in snake venom composition have been extensively documented, contributing to the diverse clinical effects observed in envenomed patients. Understanding these variations is essential for developing effective snakebite management strategies and targeted antivenom therapies. This study was prompted by the observations made by clinicians, who have noted significant variations in clinical outcomes among patients bitten by Naja mossambica in different regions of Africa, which links to the phenomenon of intra-species venom variability. We aimed to comprehensively investigate venoms from three distinct populations of N. mossambica from Eswatini, Limpopo, and KwaZulu-Natal regions in Africa in terms of their protein composition and reactivity with three commercial antivenoms (SAIMR polyvalent, EchiTAb+ICP, and Antivipmyn Africa). Methodology/Principal Findings: In contrast to previous reports, we discovered an unexpectedly high concentration of neurotoxic proteins in N. mossambica venoms (approximately 15%). The Eswatini population of Mozambique spitting cobra exhibited an increased abundance and diversity of neurotoxic proteins, including neurotoxic 3FTxs, kunitz-type inhibitors, vespryns, and mamba intestinal toxin 1. Immunochemical assessments of venom-antivenom reactivity unveiled differences, primarily related to low-abundance proteins. Notably, the reactivity of EchiTAb+ICP antivenom surpassed that of the widely used SAIMR polyvalent in serial dilution ELISA assays. Conclusions/Significance: Our findings reveal a substantial presence of neurotoxic proteins in N. mossambica venoms, challenging previous understandings of their composition. Additionally, the detection of numerous peptides aligning to uncharacterized proteins or proteins with unknown functions underscores a critical issue with existing venom protein databases, emphasizing the substantial gaps in our knowledge of snake venom protein components. This underscores the need for enhanced research in this domain. Significantly, our research highlights the superior reactivity of EchiTAb+ICP antivenom compared to SAIMR polyvalent, providing another compelling argument for its potential as an alternative to the commonly used SAIMR antivenom.

show abstract

Staphylococci, Reptiles, Amphibians, and Humans: What Are Their Relations?

Ebani

2024

Pathogens

View full text Add to dashboard Cite

Reptiles and amphibians are largely present in many environments, including domestic areas when they are kept as pet animals. They often harbor zoonotic pathogens, which can pose a serious risk of infection for humans, mainly immunocompromised individuals, the elderly, children, and pregnant women. Several studies have been carried out to verify the role of cold-blooded animals in the epidemiology of some bacteria, mainly Salmonella, whereas scarce attention has been focused on these animals as a source of staphylococci. These bacteria are often antimicrobial-resistant and they act as opportunistic pathogens, which can cause relevant infections in humans and animals, both domestic and wild. Asymptomatic reptiles and amphibians often harbor staphylococcal strains, such as Staphylococcus aureus and coagulase-negative Staphylococcus spp.; however, these bacteria have been associated with clinical conditions that usually appear in animals under stress conditions. In all cases, greater attention should also be focused on staphylococci in cold-blooded animals due to their implications in human and veterinary medicine.

show abstract

Inhibition of bacterial biofilms by the snake venom proteome

Cited by 3 publications

References 70 publications

Venom diversity in Naja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences

Venom diversity in Naja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences

Venom diversity inNaja mossambica: Insights from proteomic and immunochemical analyses reveal intraspecific differences

Staphylococci, Reptiles, Amphibians, and Humans: What Are Their Relations?

Contact Info

Product

Resources

About