Inhibition of Bacterial Attachment: Examples from the Urinary and Respiratory Tracts

C, Svambprg Edén; Åndersson, Bengt; Aniansson, G; Lindstedt, Ragnar; P, de Man; Nielsen, Allan K.; Leffler, Hakon; Wold, Agnes E.

doi:10.1007/978-3-642-74703-8_9

Cited by 9 publications

(12 citation statements)

References 63 publications

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“…10 mg ml 31 was found to completely inhibit Stx1 and Stx2 whereas lower concentrations did not a¡ect toxicity, a pattern also seen with galabiose, the minimum receptor analogue. Similar concentrations have been seen to inhibit adhesion of P-¢mbriated E. coli to uroepithelial cells [14,15]. High molecular mass pectins also exhibited inhibitory activity, albeit to a lesser degree than lower molecular mass oligosaccharides.…”

Section: Discussionmentioning

confidence: 80%

“…A moderate susceptibility of this cell line to Stx has been previously reported [14], where the ID 50 after 48 h incubation for Stx1 was 1 Wg ml 31 . This was similar to the level seen in this study (5 Wg ml 31 ).…”

Section: Discussionmentioning

confidence: 94%

“…Hydrolysis products of pectins (galacturono-oligosaccharides) have been found to block the adherence of uropathogenic P-¢mbriated E. coli to uroepithelial cells in vitro [14,15], even though they are not a part of the receptor involved in recognition. In this study, we therefore investigated the use of pectin-derived oligosaccharides as an economical alternative to globoseries receptors and/or GalK1C4GalL disaccharide for preventing gastrointestinal symptoms caused by Stx from E. coli O157:H7.…”

Section: Introductionmentioning

confidence: 99%

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Pectins and pectic-oligosaccharides inhibit Escherichia coli O157:H7 Shiga toxin as directed towards the human colonic cell line HT29

Olano-Martín

2003

FEMS Microbiology Letters

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Pectins and pectic-oligosaccharides, as derived by controlled enzymatic hydrolysis, were evaluated for their ability to interfere with the toxicity of Shiga-like toxins from Escherichia coli O157:H7. Both types of material resulted in some degree of protection but this was significantly higher (P s 0.01) with the oligosaccharide fractions (giving 90^100% cell survival, compared to 70^80% with the polymer). An effect of methylation on the protective effect was detected with lower degrees being more active. The pectic-oligosaccharides and galabiose, the minimum toxin receptor analogue, were shown to inhibit toxicity and were both protective at 10 mg ml 31 , but not at lower concentrations.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Pectins and pectic-oligosaccharides inhibit Escherichia coli O157:H7 Shiga toxin as directed towards the human colonic cell line HT29

Olano-Martín

2003

FEMS Microbiology Letters

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“…Digalacturonic acid is the principal constituent of commercial pectins, which are principally composed of polymerised (200–1000 units), partly methylated (1→4)‐linked α‐ d ‐galacturonic acid[16]. Hydrolysis products of pectins (galacturono‐oligosaccharides) have been found to block the adherence of uropathogenic P‐fimbriated E. coli to uroepithelial cells in vitro [14,15], even though they are not a part of the receptor involved in recognition. In this study, we therefore investigated the use of pectin‐derived oligosaccharides as an economical alternative to globoseries receptors and/or Galα1→4Galβ disaccharide for preventing gastrointestinal symptoms caused by Stx from E. coli O157:H7.…”

Section: Introductionmentioning

confidence: 99%

Pectins and pectic-oligosaccharides inhibitEscherichia coliO157:H7 Shiga toxin as directed towards the human colonic cell line HT29

Olano-Martín

Williams

Gibson

et al. 2003

FEMS Microbiology Letters

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Pectins and pectic-oligosaccharides, as derived by controlled enzymatic hydrolysis, were evaluated for their ability to interfere with the toxicity of Shiga-like toxins from Escherichia coli O157:H7. Both types of material resulted in some degree of protection but this was significantly higher (P>0.01) with the oligosaccharide fractions (giving 90-100% cell survival, compared to 70-80% with the polymer). An effect of methylation on the protective effect was detected with lower degrees being more active. The pectic-oligosaccharides and galabiose, the minimum toxin receptor analogue, were shown to inhibit toxicity and were both protective at 10 mg x ml(-1), but not at lower concentrations.

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“…Their saccharides may function as decoys, abrogating lectin-mediated microbial pathogen adhesion [1,2,5]. The specific binding of diverse lectins to saccharides is used for detection and isolation of gps which bear them.…”

mentioning

confidence: 99%

Differential staining of Western blots of human secreted glycoproteins from serum, milk, saliva, and seminal fluid using lectins displaying diverse sugar specifities

et al. 2005

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Human milk, serum, saliva, and seminal fluid glycoproteins (gps) nourish and protect newborn and adult tissues. Their saccharides, which resemble cell membrane components, may block pathogen adhesion and infection. In the present study, they were examined by a battery of lectins from plants, animals, and bacteria, using hemagglutination inhibition and Western blot analyses. The lectins included galactophilic ones from Aplysia gonad, Erythrina corallodendron, Maclura pomifera (MPL), peanut, and Pseudomonas aeruginosa (PA-IL); fucose-binding lectins from Pseudomonas aeruginosa (PA-IIL), Ralstonia solanacearum (RSL), and Ulex europaeus (UEA-I), and mannose/glucose-binding Con A. The results demonstrated the chosen lectin efficiency for differential analysis of human secreted gps as compared to CBB staining. They unveiled the diversity of these body fluid gp glycans (those of the milk and seminal fluid being highest): the milk gps interacted most strongly with PA-IIL, followed by RSL; the saliva gps with RSL, followed by PA-IIL and MPL; the serum gps with Con A and MPL, followed by PA-IIL and RSL, and the seminal plasma gps with RSL and MPL, followed by UEA-I and PA-IIL. The potential usage of these lectins as probes for scientific, industrial, and medical purposes, and for quality control of the desired gps is clearly indicated.

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Inhibition of Bacterial Attachment: Examples from the Urinary and Respiratory Tracts

Cited by 9 publications

References 63 publications

Pectins and pectic-oligosaccharides inhibit Escherichia coli O157:H7 Shiga toxin as directed towards the human colonic cell line HT29

Pectins and pectic-oligosaccharides inhibit Escherichia coli O157:H7 Shiga toxin as directed towards the human colonic cell line HT29

Pectins and pectic-oligosaccharides inhibitEscherichia coliO157:H7 Shiga toxin as directed towards the human colonic cell line HT29

Differential staining of Western blots of human secreted glycoproteins from serum, milk, saliva, and seminal fluid using lectins displaying diverse sugar specifities

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