Inhibition of avian leukosis virus replication by vector-based RNA interference

Chen, Mo; Granger, Adam J.; VanBrocklin, Matthew W.; Payne, William S.; Hunt, Henry D.; Zhang, Huanmin; Dodgson, Jerry B.; Holmen, Sheri L.

doi:10.1016/j.virol.2007.04.013

Cited by 36 publications

(25 citation statements)

References 44 publications

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“…Potent homologous chicken U6 and 7SK can be used for a more efficient transcription (Das et al, 2006, Bannister et al, 2007. Vectors expressing siRNAs in the context of a modified endogenous microRNA (miRNA) have been shown to be the most efficient RNAi method (Das et al, 2006;Chen et al, 2007). Das and co-workers (2006) have generated a chicken RNAi system that utilizes a chicken U6 promoter driving the expression of a modified chicken miRNA operon with embedded siRNA sequences.…”

Section: Endocrine Events In the Last Week Of Embryonic Developmentmentioning

confidence: 99%

The chicken embryo as a model for developmental endocrinology: Development of the thyrotropic, corticotropic, and somatotropic axes

Groef

Grommen

Darras

2008

Molecular and Cellular Endocrinology

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 of 37A c c e p t e d M a n u s c r i p t A c c e p t e d M a n u s c r i p t 3 The chick as a traditional model for developmental endocrinologyFor more than 2 millennia, the chicken has been a popular animal model to study embryology and developmental biology (Stern, 2005). This is due to some intrinsic advantages that the chicken embryo offers in comparison with mammalian embryonic models. Fertilized chicken eggs are low in cost and in abundant supply, and the chicken embryo is quite large and easily accessible during its entire amniotic development (Murphy & Clark, 1990). Simply by putting a number of fertilized eggs in the incubator, hundreds to thousands of embryos with a more or less synchronized development can be obtained. Furthermore, as the only endocrine connection with the mother is through hormone deposits in the egg yolk, avian embryonic development is relatively independent of changes in maternal physiology that occur after deposition of the egg, which is particularly interesting in view of endocrinological manipulations. The simplicity of direct manipulation of the embryonic endocrine interior without triggering maternal interference makes the chicken embryo a valuable model for the study of development and function of hormonal systems (Decuypere et al., 1990;Jenkins & Porter, 2004). As such, the chicken embryo is used as a model to study the cellular and subcellular differentiation and maturation of endocrine glands, ontogenic changes in the sensitivity or responsiveness of the glands and their target organs, and the development of top-down and bottom-up regulatory systems (Scanes et al., 1987). In general, the control and function of the endocrine axes are similar in birds and mammals. It should be noted, however, that the development of some endocrine systems, especially the thyroidal axis, differs considerably between precocial and altricial species. On the one hand, this limits the generalizability of the Page 4 of 37 A c c e p t e d M a n u s c r i p t 4 chicken embryo model to other avian speci...

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Section: Endocrine Events In the Last Week Of Embryonic Developmentmentioning

confidence: 99%

The chicken embryo as a model for developmental endocrinology: Development of the thyrotropic, corticotropic, and somatotropic axes

Groef

Grommen

Darras

2008

Molecular and Cellular Endocrinology

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“…4 Chemically synthesized siRNAs and short-hairpin RNA expression plasmids, which are sequence-specific in targeting mRNAs, are common methods that mimic Dicer cleavage. 5 However, siRNAs are susceptible to nuclease destruction and cannot penetrate the cell membrane because of their highly charged backbone. An effective delivery system, in which siRNA are incorporated in carriers, could protect and transport siRNA to the cytoplasm of target cells.…”

Section: Introductionmentioning

confidence: 99%

Low molecular weight chitosan conjugated with folate for siRNA delivery in vitro: optimization studies

Fernandes¹,

Qiu²,

Winnik³

et al. 2012

IJN

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Abstract:The low transfection efficiency of chitosan is one of its drawbacks as a gene delivery carrier. Low molecular weight chitosan may help to form small-sized polymer-DNA or small interfering RNA (siRNA) complexes. Folate conjugation may improve gene transfection efficiency because of the promoted uptake of folate receptor-bearing cells. In the present study, chitosan was conjugated with folate and investigated for its efficacy as a delivery vector for siRNA in vitro. We demonstrate that the molecular weight of chitosan has a major influence on its biological and physicochemical properties, and very low molecular weight chitosan (below 10 kDa) has difficulty in forming stable complexes with siRNA. In this study, chitosan 25 kDa and 50 kDa completely absorbed siRNA and formed nanoparticles (#220 nm) at a chitosan to siRNA weight ratio of 50:1. The introduction of a folate ligand onto chitosan decreased nanoparticle toxicity. Compared with chitosan-siRNA, folate-chitosan-siRNA nanoparticles improved gene silencing transfection efficiency. Therefore, folate-chitosan shows potential as a viable candidate vector for safe and efficient siRNA delivery.

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“…Hu et al [7] adopted siRNA designed against the ALV gag gene and demonstrated significantly reduced virus replication. Chen et al [8] indicated that ALV-B replication was significantly inhibited after knockdown of the ALV-B tvb and env genes.…”

Section: Introductionmentioning

confidence: 99%

Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs

Meng

Zhang

Wang

et al. 2011

Virol J

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BackgroundAvian leukosis virus (ALV) is a major infectious disease that impacts the poultry industry worldwide. Despite intensive efforts, no effective vaccine has been developed against ALV because of mutations that lead to resistant forms. Therefore, there is a dire need to develop antiviral agents for the treatment of ALV infections and RNA interference (RNAi) is considered an effective antiviral strategy.ResultsIn this study, the avian leukosis virus subgroup J (ALV-J) proviral genome, including the gag genes, were treated as targets for RNAi. Four pairs of miRNA sequences were designed and synthesized that targeted different regions of the gag gene. The screened target (i.e., the gag genes) was shown to effectively suppress the replication of ALV-J by 19.0-77.3%. To avoid the generation of escape variants during virus infection, expression vectors of multi-target miRNAs were constructed using the multi-target serial strategy (against different regions of the gag, pol, and env genes). Multi-target miRNAs were shown to play a synergistic role in the inhibition of ALV-J replication, with an inhibition efficiency of viral replication ranging from 85.0-91.2%.ConclusionThe strategy of multi-target miRNAs might be an effective method for inhibiting ALV replication and the acquisition of resistant mutations.

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Inhibition of avian leukosis virus replication by vector-based RNA interference

Cited by 36 publications

References 44 publications

The chicken embryo as a model for developmental endocrinology: Development of the thyrotropic, corticotropic, and somatotropic axes

The chicken embryo as a model for developmental endocrinology: Development of the thyrotropic, corticotropic, and somatotropic axes

Low molecular weight chitosan conjugated with folate for siRNA delivery in vitro: optimization studies

Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs

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