Inhibition of autophagy by chloroquine potentiates synergistically anti-cancer property of artemisinin by promoting ROS dependent apoptosis

Ganguli, Arnab; Choudhury, Diptiman; Datta, Satabdi; Bhattacharya, Surela; Chakrabarti, Gopal

doi:10.1016/j.biochi.2014.10.001

Cited by 86 publications

(71 citation statements)

References 38 publications

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“…This is consistent with the results in malaria, cancer cells and yeast, where artemisinin also induced ROS accumulation (Li et al, 2005;Gao et al, 2013;Antoine et al, 2014;Ganguli et al, 2014;Gopalakrishnan and Kumar, 2015). Generation of ROS by artemisinin in these organisms is thought to be mediated by its interference with the electron transfer chain (ETC) (Li et al, 2005;Wang et al, 2010;Mercer et al, 2011).…”

Section: Discussionsupporting

confidence: 86%

“…It has been postulated that artemisinin has multiple targets such as sarco/endoplasmic reticulum Ca 2þ -ATPases (SERCAs), PfATP6, heme-and non-heme-containing proteins, as well as ROS (O'Neill et al, 2010;Crespo-Ortiz and Wei, 2012). Recent studies highlighted the importance of ROS-mediated damage induced by artemisinin in leading to cancer cell apoptosis and parasite death (Gao et al, 2013;Antoine et al, 2014;Ganguli et al, 2014;Gopalakrishnan and Kumar, 2015).…”

Section: Discussionmentioning

confidence: 99%

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Mechanism of artemisinin phytotoxicity action: Induction of reactive oxygen species and cell death in lettuce seedlings

Yan

Wang

Ding

et al. 2015

Plant Physiology and Biochemistry

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Mechanism of artemisinin phytotoxicity action: Induction of reactive oxygen species and cell death in lettuce seedlings

Yan

Wang

Ding

et al. 2015

Plant Physiology and Biochemistry

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“…34 Experimental cells were stained with JC-1 (1 µg/mL) for 15 min at 37°C and subjected to flowcytometric analysis in BD FACScalibur instrument. 35 …”

Section: Measurement Of Mmpmentioning

confidence: 99%

Paclitaxel resistance development is associated with biphasic changes in reactive oxygen species, mitochondrial membrane potential and autophagy with elevated energy production capacity in lung cancer cells: A chronological study

et al. 2017

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Paclitaxel (Tx) is one of the first-line chemotherapeutic drugs used against lung cancer, but acquired resistance to this drug is a major challenge against successful chemotherapy. In this work, we have focused on the chronological changes of various cellular parameters and associated effect on Tx (10 nM) resistance development in A549 cell line. It was observed, at initial stage, the cell death percentage due to drug treatment had increased up to 20 days, and thereafter, it started declining and became completely resistant by 40 days. Expressions of βIII tubulin and drug efflux pumps also increased over the period of resistance development. Changes in cellular autophagy and reactive oxygen species generation showed a biphasic pattern and increased gradually over the course of upto 20 days, thereafter declined gradually; however, their levels remained higher than untreated cells when resistance was acquired. Increase in extracellular acidification rates and oxygen consumption rates was found to be directly correlated with acquisition of resistance. The depolarisation of mitochondrial membrane potential was also biphasic; first, it increased with increase of cell death up to 20 days, thereafter, it gradually decreased to normal level along with resistance development. Increase in activity of catalase, glutathione peroxidase and glutathione content over these periods may attribute in bringing down the reactive oxygen species levels and normalisation of mitochondrial membrane potential in spite of comparatively higher reactive oxygen species production by the Tx-resistant cells.

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“…Along with ROS, nitric oxide (NO) is involved in a wide range of toxic oxidative reactions [8]; therefore, inhibiting the release of NO from macrophages is a potential method of controlling inflammation [9, 10]. It has been demonstrated that oxidative stress is involved in the pathogenesis of ALD, as ROS generation has been observed in alcohol-exposed cultured cells and in alcohol-exposed mouse embryos [11].…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

Liu

Wang

et al. 2017

Oxidative Medicine and Cellular Longevity

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In the present study, the components of A. cinnamomea (AC) mycelia were systematically analyzed. Subsequently, its hepatoprotective effects and the underlying mechanisms were explored using a mouse model of acute alcohol-induced liver injury. AC contained 25 types of fatty acid, 16 types of amino acid, 3 types of nucleotide, and 8 types of mineral. The hepatoprotective effects were observed after 2 weeks of AC treatment at doses of 75 mg/kg, 225 mg/kg, and 675 mg/kg in the mouse model. These effects were indicated by the changes in the levels of aspartate aminotransferase, alanine aminotransferase, several oxidation-related factors, and inflammatory cytokines in serum and/or liver samples. AC reduced the incidence rate of necrosis, inflammatory infiltration, fatty droplets formation, and cell apoptosis in liver detecting via histological and TUNEL assay. In addition, AC reduced the expression of cleaved caspase-3, -8, and -9 and the levels of phosphor-protein kinase B (Akt) and phosphor-nuclear factor-κB (NF-κB) in the liver samples. Collectively, AC-mediated hepatoprotective effects in a mouse model of acute alcohol-induced liver injury are the result of reduction in oxidative stress. This may be associated with Akt/NF-κB signaling. These results provide valuable evidence to support the use of A. cinnamomea as a functional food and/or medicine.

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Inhibition of autophagy by chloroquine potentiates synergistically anti-cancer property of artemisinin by promoting ROS dependent apoptosis

Cited by 86 publications

References 38 publications

Mechanism of artemisinin phytotoxicity action: Induction of reactive oxygen species and cell death in lettuce seedlings

Mechanism of artemisinin phytotoxicity action: Induction of reactive oxygen species and cell death in lettuce seedlings

Paclitaxel resistance development is associated with biphasic changes in reactive oxygen species, mitochondrial membrane potential and autophagy with elevated energy production capacity in lung cancer cells: A chronological study

Hepatoprotective Effects of Antrodia cinnamomea: The Modulation of Oxidative Stress Signaling in a Mouse Model of Alcohol‐Induced Acute Liver Injury

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