Inhibition of archenteron formation by the inhibitors of prolyl hydroxylase in sea urchin embryos

Mizoguchi, Hazime; Yasumasu, Ikuo

doi:10.1016/0045-6039(83)90032-5

Cited by 18 publications

(10 citation statements)

References 10 publications

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“…These morphological changes are probably supported by the synthesis of collagen in the gut during these stages. This possibility is supported by reports that treatment of embryos with a, a'-dipyridyl from before the midgastrula stage inhibits the gut formation and that similar treatment after the gut has formed results in breakdown of the gut (19,21). Treatment with a, a'-dipyridyl from the late gastrula stage does not destroy the gut itself but inhibits further morphological changes (19,21).…”

Section: Discussionsupporting

confidence: 65%

“…According to the classical definition, collagen is a hot acid-extractable protein that contains hydroxyproline and hydroxylysine residues (1,12,27). Exposure of embryos to ''C-proline results in its incorporation into hot acid-extractable proteins that contain 14C-hydroxyproline residues (6,7,(17)(18)(19)(20)(21)23). The percentage ratio of 14C-hydroxyproline residues to 14C-proline residues is about 4% at most in sea urchin embryos ( 6, 7, 17-21,23), which is rnarkedly lower than in other organisms (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…Despite all these lines of circumstantial evidence, no hydroxyproline residues have yet been found in sea urchin embryos, probably because of the low hydroxylation ratio (6,7,(17)(18)(19)(20)(21)23). However, recently proteins that cross-reacted with antibodies raised against mammalian collagens were found on the cell surface of sea urchin embryos in the blastula and pluteus stages (30).…”

Section: Introductionmentioning

confidence: 99%

“…In sea urchin embryos, collagen-like proteins are reported to be synthesized after the blastula stage (6, 7,[17][18][19][20][21]23). According to the classical definition, collagen is a hot acid-extractable protein that contains hydroxyproline and hydroxylysine residues (1,12,27).…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Collagen‐Like Proteins in Embryonic Organs of the Sea Urchin, Hemicentrotus pulcherrimus

1989

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In sea urchin embryos exposed to ''C-proline at 20°C for 3 hr at the gastrula, prism or pluteus stage, 14C-radioactivity was found in hot acid-extractable proteins, in which more than 4% of the radioactivity was detectable in hydroxyproline residues. In these embryos, 14C-radioactivity in collagen-like proteins was found in the archenteron, spicule and embryo-wall cells. The rate of synthesis of collagen-like proteins was highest in the archenteron in the mid-gastrula stage, in the embryo-wall cells in the prism stage and in the spicule in the pluteus stage. The rate of synthesis decreased in the archenteron and increased in ernbryo-wall cells in the period between the mid-and late-gastrula stages, when the rate of synthesis in the spicule was quite low. Thereafter, the rate decreased slightly in the embryo-wall cells, was maintained in archenteron and increased markedly in the spicule. The rates of synthesis of collagen-like proteins are high in these embryonic organs at stages at which development and growth respectively, occur in embryos. Therefore, synthesis of collagen-like proteins probably supports morphogenesis in these embryonic organs.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis of Collagen‐Like Proteins in Embryonic Organs of the Sea Urchin, Hemicentrotus pulcherrimus

1989

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“…DP is a competitive inhibitor of prolyl-hydroxylase, which catalyses a critical step required for formation of the collagen triple helix (Kivirikko et al, 1989). DP interferes with collagen biosynthesis in vertebrate and invertebrate models, including Hydra (Kawano et al, 2005;Mizoguchi and Yasumasu, 1983;Sarras et al, 1991b;Sarras et al, 1993). Two sets of experiments were performed to test the effect of DP on bud formation.…”

Section: Biosynthesis Of New Collagens Is Not Required For Early Stagmentioning

confidence: 99%

In vivo imaging of basement membrane movement: ECM patterning shapesHydrapolyps

Aufschnaiter

Zamir

Little

et al. 2011

Journal of Cell Science

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SummaryGrowth and morphogenesis during embryonic development, asexual reproduction and regeneration require extensive remodeling of the extracellular matrix (ECM). We used the simple metazoan Hydra to examine the fate of ECM during tissue morphogenesis and asexual budding. In growing Hydra, epithelial cells constantly move towards the extremities of the animal and into outgrowing buds. It is not known, whether these tissue movements involve epithelial migration relative to the underlying matrix or whether cells and ECM are displaced as a composite structure. Furthermore, it is unclear, how the ECM is remodeled to adapt to the shape of developing buds and tentacles. To address these questions, we used a new in vivo labeling technique for Hydra collagen-1 and laminin, and tracked the fate of ECM in all body regions of the animal. Our results reveal that Hydra 'tissue movements' are largely displacements of epithelial cells together with associated ECM. By contrast, during the evagination of buds and tentacles, extensive movement of epithelial cells relative to the matrix is observed, together with local ECM remodeling. These findings provide new insights into the nature of growth and morphogenesis in epithelial tissues.

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Ultrastructure and synthesis of the extracellular matrix ofPisaster ochraceus embryos preserved by freeze substitution

1997

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Inhibition of archenteron formation by the inhibitors of prolyl hydroxylase in sea urchin embryos

Cited by 18 publications

References 10 publications

Synthesis of Collagen‐Like Proteins in Embryonic Organs of the Sea Urchin, Hemicentrotus pulcherrimus

Synthesis of Collagen‐Like Proteins in Embryonic Organs of the Sea Urchin, Hemicentrotus pulcherrimus

In vivo imaging of basement membrane movement: ECM patterning shapesHydrapolyps

Ultrastructure and synthesis of the extracellular matrix ofPisaster ochraceus embryos preserved by freeze substitution

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