Inhibition of arachidonic acid metabolism and pro-inflammatory cytokine production by Bruguiera gymnorrhiza leaf

Barik, Rajib; Sarkar, Ratul; Biswas, Pritam; Pattnaik, Ashok Kumar; Samanta, Susanta Kumar; Manisenthilkumar, KT; Pal, Mahesh; Karmakar, Sanmoy; Sen, Tuhinadri

doi:10.1007/s13596-013-0103-9

Cited by 9 publications

(8 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The test compounds were preincubated with human peripheral blood mononuclear cells for 30 min at 37°C and then stimulated with lipopolysaccharide ( E. coli : B4; 1 μg/ml) for 18 h (37°C; 5% CO2). Cytokine (tumour necrosis factor‐alpha (TNF‐α), interleukin‐6 (IL‐6) and interleukin‐1 beta (IL‐1β)) concentration was estimated using ELISA kits (Cayman Chemical, Ann Arbor, USA) [52]…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial properties ofKalanchoe blossfeldiana: a focus on drug resistance with particular reference to quorum sensing-mediated bacterial biofilm formation

Sarkar

Mondal

Bera

et al. 2015

Journal of Pharmacy and Pharmacology

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Antimicrobial properties ofKalanchoe blossfeldiana: a focus on drug resistance with particular reference to quorum sensing-mediated bacterial biofilm formation

Sarkar

Mondal

Bera

et al. 2015

Journal of Pharmacy and Pharmacology

Self Cite

View full text Add to dashboard Cite

show abstract

“…As evident from our earlier studies, the anti-inflammatory activity of BRG was found to be mediated through dual COX2 and 5-LOX enzyme inhibition along with inhibition of TNF-α production. [ 4 ] As an alternative to the existing NSAIDS, dual COX-LOX inhibitors have been reported to show better GI and cardiovascular tolerance. Thus, in the present investigation an attempt was made to isolate the bioactive component (s) from this mangrove medicinal plant.…”

Section: Discussionmentioning

confidence: 99%

“…In a similar attempt, we have also reported that the methanolic extract of the Bruguiera gymnorrhiza (BRG) (Family: Rhizophoraceae ) leaves displayed promising anti-inflammatory activity in different in vitro and in vivo models. [ 4 ]…”

Section: Introductionmentioning

confidence: 99%

“…However, these drugs have serious limitations owing to their various adverse effects. [ 3 4 ] Conventional nonsteroidal anti-inflammatory drugs (NSAIDs), which are potentially unsafe for the gastrointestinal (GI) tract and the liver, mediate their anti-inflammatory effects by blocking the COX enzymes, thereby causing a shift of the arachidonic acid metabolism to the 5-LOX pathway, whereas the dual inhibitors (owing to the blockade of both the enzymes) display reduced toxic manifestation. [ 5 ]…”

Section: Introductionmentioning

confidence: 99%

“…Based on the observed dual inhibitory activity of BRG,[ 4 ] the present investigation was carried out for isolation and characterization of the bioactive component. Efforts were also made to elucidate the possible mechanism of action of the purified component.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

5,7-dihydroxy-2-(3-hydroxy-4, 5-dimethoxy-phenyl)-chromen-4-one-a flavone from Bruguiera gymnorrhiza displaying anti-inflammatory properties

et al. 2016

Self Cite

View full text Add to dashboard Cite

Objective:Bruguiera gymnorrhiza (BRG) (L.) Lamk (Rhizophoraceae), a mangrove species, is widely distributed in the Pacific region, eastern Africa, Indian subcontinent, and subtropical Australia. The leaves of this plant are traditionally used for treating burns and inflammatory lesions. This study isolates the bioactive compound from the methanol extract of BRG leaves and evaluates the possible mechanisms of anti-inflammatory activity involved.Materials and Methods:Bioassay-guided fractionation of BRG was performed to identify the bioactive fraction (displaying inhibition of cyclooxygenase 2 [COX2] - 5-lipoxygenase (5-LOX) activities and tumor necrosis factor-alpha (TNF-α) production at the tested concentrations of 100 and 10 μg/ml). The fractionation was performed by solvent extraction and preparative high-performance liquid chromatography. The bioactive compound was characterized by ultraviolet–visible, liquid chromatography–mass spectrometry and nuclear magnetic resonance spectroscopy. The antioxidant potential was evaluated by electron spin resonance spectrum of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical at 250 μM. The effect of the compound was also studied on TNF-α converting enzyme and nuclear factor kappa B (NF-κB) activities at the concentrations 100, 10 and 1 μg/ml.Results:Bioassay-guided purification of BRG revealed the presence of a flavone (5,7-dihydroxy-2- [3-hydroxy-4,5-dimethoxy-phenyl]-chromen-4-one) of molecular weight 330Da. It demonstrated more than 80% inhibition against COX2, 5-LOX activities and TNF-α production at 100 μg/ml. It also displayed 40% inhibition against DPPH radical at the tested concentration along with 23.1% inhibition of NF-κB activity at 100 μg/ml.Conclusions:The isolated methoxy-flavone may play a predominant role in the anti-inflammatory properties displayed by BRG leaves. Such activity may involve multiple mechanisms, namely (a) modulation of oxidative stress (b) inhibition of arachidonic acid metabolism and (c) downregulation of pro-inflammatory cytokines probably through NF-κB inhibition.

show abstract

Attenuation of COX-2 enzyme by modulating H2O2-mediated NF-κB signaling pathway by monoamine oxidase inhibitor (MAOI): a further study on the reprofiling of MAOI in acute inflammation

Sur¹,

Mondal

Balaraman

et al. 2023

Inflammopharmacol

View full text Add to dashboard Cite

Inhibition of arachidonic acid metabolism and pro-inflammatory cytokine production by Bruguiera gymnorrhiza leaf

Cited by 9 publications

References 18 publications

Antimicrobial properties ofKalanchoe blossfeldiana: a focus on drug resistance with particular reference to quorum sensing-mediated bacterial biofilm formation

Antimicrobial properties ofKalanchoe blossfeldiana: a focus on drug resistance with particular reference to quorum sensing-mediated bacterial biofilm formation

5,7-dihydroxy-2-(3-hydroxy-4, 5-dimethoxy-phenyl)-chromen-4-one-a flavone from Bruguiera gymnorrhiza displaying anti-inflammatory properties

Attenuation of COX-2 enzyme by modulating H2O2-mediated NF-κB signaling pathway by monoamine oxidase inhibitor (MAOI): a further study on the reprofiling of MAOI in acute inflammation

Contact Info

Product

Resources

About