Inhibition of antigen- and mitogen-induced human lymphocyte proliferation by gold compounds.

Lipsky, Peter E.; Ziff, Morris

doi:10.1172/jci108660

Cited by 186 publications

(49 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data presented here indicate that these concentrations of GST can inhibit lymphocyte activation in vitro. Inhibition occurred even in the presence of optimal concentrations of mitogen, although Lipsky & Ziff (1977) found that inhibition of human lymphocyte activation was more effective when sub-optimal mitogen stimulation was used. Inhibition of both Con A-and LPS-induced activation indicates inhibition of a function necessary for both B and T lymphocyte activation.…”

Section: Discussionmentioning

confidence: 97%

“…Lies, Cardin & Paulus (1977) found that inhibitory effects were not observed if GST was added later than 6 h from the start of the culture. Lipsky & Ziff (1977) also suggested that an early stage of activation was affected, although failure to add GST until 18 h appeared to have no less an effect where less than 100 Mg ml -l GST was used.…”

Section: Discussionmentioning

confidence: 99%

“…Lipsky & Ziff (1977) and Lies et al (1977) reported that thiomalate did not inhibit human lymphocyte function although McCormack & Palmer (1980) found that it did at concentrations of 19 igml-1 and above, whilst GST and sodium choroaurate needed to be at 50 yg ml-l. Use of sodium chloroaurate as a control for the action of thiol-free metal may be criticized on the ground that its oxidation state is + 3 whereas that of GST is + 1. However, in the presence of a three fold excess of thiol it is suggested that gold (III) will behave as gold (I) (Sadler, 1982).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of lymphocyte activation by gold sodium thiomalate

Hopkins

Jayson

Zeil

1983

British J Pharmacology

View full text Add to dashboard Cite

Activation of lymphoid cells by both T and B cell mitogens was inhibited by gold sodium thiomalate (GST). The action of GST did not appear to be exerted at early stages of lymphocyte activation. Inhibition by GST was sustained throughout 4 days of culture. The inhibitory effect of GST was reduced at low serum concentrations. Sodium thiomalate and sodium chloroaurate were also able to inhibit lymphocyte activation.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of lymphocyte activation by gold sodium thiomalate

Hopkins

Jayson

Zeil

1983

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…It has also been reported that these compounds suppress accessory cell function of macrophages in local immune responses (24). It may be possible, therefore, that gold compounds regulate local fibroblast proliferation through alteration of macrophage capacity for induction of proliferation of fibroblasts or through inhibition of IL-1 secretion from macrophages.…”

Section: Discussionmentioning

confidence: 99%

Low‐dose gold compounds inhibit fibroblast proliferation and do not affect interleukin‐1 secretion by macrophages

Matsubara

Saegusa

Hirohata

1988

Arthritis & Rheumatism

View full text Add to dashboard Cite

We examined the effect of low concentrations of gold compounds on the proliferation of human fibroblasts. Gold sodium thiomalate (GST) inhibited both basal and interleukin-l-induced tritiated thymidine incorporation into fibroblasts in a dose-and timedependent manner. Significant inhibition was observed at the level of 5 pg/ml GST, and >SO% inhibition was attained at 10 pg/ml. These concentrations are attainable in the serum of treated patients. Similar inhibition was observed when < I pglml auranofin, which is also within a serum-attainable range, was added. Low concentrations of GST (0-10 pg/ml) did not affect interleukin-1 secretion from lipopolysaccharide-stimulated human mononuclear phagocytes (M Q,) when assessed by both human fibroblast and C3H/HeJ mouse thymocyte proliferation assays. When MQ, precultured for 48 hours with GST (0-10 pglml) were added to the fibroblast culture in the presence or absence of lipopolysaccharide, there was no significant inhibition of MQ,-induced DNA synthesis of fibroblasts. In contrast, when fibroblasts were precultured with GST (0-10 pg/ml) for 48 hours and freshly separated M 4 were added, significant inhibition was observed in MQ,-induced fibroblast proliferation at 5 pglml. These results suggest that low concentrations of GST directly cause a reduction of fibroblast proliferation, but do not affect the capability of MQ, for induction of fibroblast proliferation. There-

show abstract

“…Aurothiomalate (20) and auranofin (19) can inhibit the functional capacity of monocytes to induce T lymphocyte proliferation in vitro. Both …”

Section: Figure 1 Structuresofsome Widely Usedgold Drugsand the Metabmentioning

confidence: 99%

Gold drugs: Mechanism of action and toxicity

1996

View full text Add to dashboard Cite

Gold drugs are still amongst the most efficacious for the treatment of rheumatoid arthritis. Their mechanism of action, as well as the molecular basis of their side-effects, remain poorly understood. Current theories are reviewed, including recent potential breakthroughs. The interaction of gold(III) with peptides and proteins and its immunochemical implications are discussed.Ninety elements occur naturally on earth of which 9 are radioactive. Eighty-one elements are therefore potentially available to support life, of which 61 are metals. It is believed that about 25 are essential for human life but our knowledge of the biochemistry of several of these (e.g.V; Ni, Sn) is poor. In general, therefore, there is enormous scope for the use of inorganic compounds in medicine, and a need for research in this area. Metals currently used in medicine include gold in antiarthritic agents, platinum in anticancer drugs, lithium for manic depression, bismuth in anti-ulcer drugs, and silver and mercury in antimicrobial agents (1). The effectiveness of ruthenium complexes as anti-metastatic agents with potential use in cancer therapy is currently receiving much attention (2). However, the potential of inorganic metal compounds as drugs has yet to be fully explored. With the exception of platinum, which has attracted the most research efforts because of its importance in the therapy of some types of cancer, and which is now generally thought to be effective by interaction with DNA, the mechanism of action of other inorganic drugs is mostly unknown.One of the problems of inorganic drugs is their side-effects. For gold drugs used in the therapy of * Hypersensitivity is an adaptive immune response occurring in an exaggerated or inappropriate form causing tissue damage. It is a characteristic of the individual and is manifested on second contact with the particular antigen (in this case gold drugs). Delayed-type hypersensitivity takes more than 12 hours to develop and is mediated primarily by T cell and macrophages.

show abstract

Inhibition of antigen- and mitogen-induced human lymphocyte proliferation by gold compounds.

Abstract: A B S T R A C T Gold sodium thiomalate (GST)

Cited by 186 publications

References 36 publications

Inhibition of lymphocyte activation by gold sodium thiomalate

Inhibition of lymphocyte activation by gold sodium thiomalate

Low‐dose gold compounds inhibit fibroblast proliferation and do not affect interleukin‐1 secretion by macrophages

Gold drugs: Mechanism of action and toxicity

Contact Info

Product

Resources

About