Inhibition of angiogenesis by chitooligosaccharides with specific degrees of acetylation and polymerization

Wu, Huixing; Aam, Berit Bjugan; Wang, Wenxia; Norberg, Anne Line; Sørlie, Morten; Eijsink, Vincent G. H.; Du, Yifeng

doi:10.1016/j.carbpol.2012.03.037

Cited by 51 publications

(26 citation statements)

References 34 publications

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“…According to one research organic matrix composition of chiton Acanthopleura villantii shell were investigated: the chitin protein ratio is 6 to 9, which is more than a hundred times greater than the amount that is found in the shells of other mollusks [15]. Wu et al showed effects of different concentrations of chitooligosaccharides on chorioallantoic membrane, that upon which causes reduce the formation and growth of vessels in the CAM model [16]. These results are consistent with our results in this research, because of the presence of chitin in the shell of chiton and its anti angiogenesis effect in CAM model.…”

Section: Discussionmentioning

confidence: 99%

“…Zhang and Kim, for the first time, investigated effects of marine chitooligosaccharides on the activation and expression of matrix metalloproteinase 2 in human skin fibroblasts and showed that marine chitooligosaccharides inhibit expression and activity of matrix metalloproteinase 2 [17]. Because of presence chitin and chitosan in molluska [18,19] and anti angiogenesis effects of chitooligosaccharides [16], probably presence chitin and chitosan in chiton shell is one of the reasons for its decreasing effect on angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Alcoholic Extract of Persian Gulf Chiton (lamyi) Shell on Angiogenesis in Chick Chorioallantoic Membrane

Javan-Jaafari-Bojnourdi

Baharara

2015

Zahedan J Res Med Sci

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Background: Inhibition of angiogenesis is a major target in control and treatment of many disorders depend of angiogenesis. Chiton is a marine mollusk and it can be useful for treatment of diseases because of exist many chemical compositions in its. We investigated the effects of shell extract of chiton on angiogenesis in chorioallantoic membrane. Materials and Methods:In this experimental study, we used 30 Ross fertilized eggs that were divided into 3 random groups: control, sham-exposed (treated by dimethyle sulfoxide: DMSO) and experimental (treated with shell extract). In 2nd day, a window was opened on eggs in the sterile condition. Later, in 8nd day, a gelatin sponge appeared on chorioallantoic membrane and was soaked with 10 μL extract in treatment group and 10 μL DMSO in the sham-exposed group. In 12nd day, chorioallantoic membranes (CAMs) were photographed by research photo stereomicroscope in all cases. The numbers and lengths of vessels around the sponges were measured and compared with each other by SPSS-16 software and ANOVA (p<0.05). Results: The mean number of vessels (10.34±1.85) and length of vessels (13.12±2.04 mm) in the control group and mean number of vessels (9.97±1.38) and length of vessels (13.42±1.08 mm) in sham-exposed group was not any significant differences (p>0.05). There was a significant decrease in mean number of vessels (6.06±1.36) and length of vessels (9.76±1.21 mm) in experimental group. Conclusion: It seems shell extract of Chiton lamyican decreases the number and length of vessels around treated area so it can be used as inhibitory agent in treatment of angiogenesis dependent disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Effect of Alcoholic Extract of Persian Gulf Chiton (lamyi) Shell on Angiogenesis in Chick Chorioallantoic Membrane

Javan-Jaafari-Bojnourdi

Baharara

2015

Zahedan J Res Med Sci

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“…Chitooligosaccharides (COS), produced from chitin, becomes the focus because its characteristics like easy solubility and high absorptivity are apparently better than polymer sugar. Currently, few COS reports focused on its application in radiosensitization of cancer cell [9,10] . This research aims to investigate the elevating sensibility of human lung cancer line HepG2 treated with COS to radiotherapy, and to verify the auxiliary synergism of COS on radiotherapy in order to explore the therapy for cancer with better efficacy and lower damage.…”

Section: Introductionmentioning

confidence: 99%

Anti-proliferation and radiosensitization effects of chitooligosaccharides on human lung cancer line HepG2

Han

Cui

You

et al. 2015

Asian Pacific Journal of Tropical Medicine

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“…The inhibitory effects of chitooligosaccharides, as antioxidant compounds on angiogenesis, had received some attention (Wang et al, 2007 andWu et al, 2008). When tumor cells reached a certain size, new capillary blood vessels was formed from already existing blood vessels.…”

Section: Introductionmentioning

confidence: 99%

“…When tumor cells reached a certain size, new capillary blood vessels was formed from already existing blood vessels. Chitooligosaccharides inhibited the growth of tumor cells through influence immuno-enhancing effects and also by inhibiting angiogenesis (Wu et al, 2012). In intestinal intraepithelial lymphocytes, chitooligosaccharides improved the natural killer activity and decreased tumor growth in mice (Maeda and Kimura, 2004).…”

Section: Introductionmentioning

confidence: 99%

Evaluation Antioxidant and cytotoxic activities of novel chitooligosaccharides prepared from chitosan via enzymatic hydrolysis and ultrafiltration

2017

J App Pharm Sci

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Our work was designed to evaluate antioxidant (free radical scavenging and reducing power) and cytotoxicity activities of chitooligosaccharides with different molecular weights. The antioxidant activities in vitro were assessed by three separated methods, including DPPH (1,1-diphenyl-2-picrylhydrazyl radical) and ABTS (2,2-azinobis (3-methyl-benzothiazoline-6-sulfonic) free radical-scavenging assay systems and ferric reducing power (FRAP). Results were compared with that of trolox which was used as a reference compound. There was correlation between antioxidant activity and molecular weight of chitooligosaccharides. In general, lower molecular weights showed better activity than higher one. Chitooligosaccharides with molecular weight lower than 1 KDa showed highest antioxidant activity in the all tested methods (86.97to 93.56 µgTE/mg). Chitooligosaccharides with molecular weight less than 1.0 KDa group was analyzed by high-performance liquid chromatograph (HPLC). It mainly contained N-acetylglucose amine (36.7 %), chitobiose (29.1 %) and chitotetrose (23.51 %). The chitooligosaccharides groups were also subjected to investigate cytotoxic activity on three different carcinoma cells lines (HEPG2, HCT and MCF7). Cytotoxicity was evaluated by sulfohodamine B (SRB) cell viability assay in vitro. Chitooligosaccharides with molecular weight 10 to 100 KDa showed IC50 1.564, 1.84 and 2.208 µg/ml against HEPG2, HCT and MCF7 cells, respectively. While Chitooligosaccharides with molecular weight 1.0 to 10 KDa showed IC50 12.948 and 11.952µg/ml against HEPG2 and MCF7 cells, respectively. They caused 75 to 80% inhibition of the tested cells. Thus, the chitooligosaccarides had valuable antioxidant and cytotoxicity, which related to their molecular weights. Chitooligosaccharides may be used as a functional food ingredient in pharmaceutical and food industries future.

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Inhibition of angiogenesis by chitooligosaccharides with specific degrees of acetylation and polymerization

Cited by 51 publications

References 34 publications

The Effect of Alcoholic Extract of Persian Gulf Chiton (lamyi) Shell on Angiogenesis in Chick Chorioallantoic Membrane

The Effect of Alcoholic Extract of Persian Gulf Chiton (lamyi) Shell on Angiogenesis in Chick Chorioallantoic Membrane

Anti-proliferation and radiosensitization effects of chitooligosaccharides on human lung cancer line HepG2

Evaluation Antioxidant and cytotoxic activities of novel chitooligosaccharides prepared from chitosan via enzymatic hydrolysis and ultrafiltration

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