Inhibition of anchorage-independent growth and lung metastasis of A549 lung carcinoma cells by IκBβ

Jiang, Yixing; Chen, Long; Yie, Ting-An; Rom, William N.; Cheng, Hua; Tchou-Wong, Kam Meng

doi:10.1038/sj.onc.1204293

Cited by 40 publications

(34 citation statements)

References 29 publications

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“…Clonogenic assay of RASSF8-transfected and control A549 cells revealed no significant effect of RASSF8 protein overexpression on cancer cell viability, since colony numbers were similar. However, anchorage-independent growth in soft agar, which is correlated with tumor progression and metastasis formation (Nikiforov et al, 1996;Clezardin, 1998;Jiang et al, 2001;Malaney and Daly, 2001;Nakanishi et al, 2002), was significantly reduced in the RASSF8-transfected A549 cells (Figure 3b and c). Moreover, RASSF8 transfection inhibited clonogenic activity in NCI-H520 cancer cell line.…”

Section: Discussionmentioning

confidence: 91%

Identification of RASSF8 as a candidate lung tumor suppressor gene

et al. 2006

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Section: Discussionmentioning

confidence: 91%

Identification of RASSF8 as a candidate lung tumor suppressor gene

et al. 2006

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“…In addition, expression of the super-repressor form of IkBa in human melanoma and ovarian cancer cells correlates with reduced VEGF and interleukin (IL)-8 expression and blocks growth, angiogenesis and metastasis of tumor xenografts (Huang et al, 2000b). Moreover, NF-kB inhibition by overexpression of IkBb in lung cancer cells suppressed their ability to form metastasis (Jiang et al, 2001). It should be noted that these studies often demonstrate that inhibition of NF-kB also causes reduction of tumor growth, so that it is difficult to demonstrate absolutely that NF-kB directly controls angiogenesis and metastasis.…”

Section: Apoptosis Evasionmentioning

confidence: 99%

Nuclear factor-κB and inhibitor of κB kinase pathways in oncogenic initiation and progression

2006

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“…A549 cells have an activating mutation in k-ras (homozygous at codon 12) that leads to overamplification of the EGF signal (11). We asked whether the in vivo -induced gene expression patterns in A549 were reflective in part of its k-ras genotype and whether these patterns had any correspondence with what may be found in human tumors.…”

Section: A549 Tumors Over In Vitro Cultured A549 Cells Exhibit a Signmentioning

confidence: 99%

Analysis of Tumor-Host Interactions by Gene Expression Profiling of Lung Adenocarcinoma Xenografts Identifies Genes Involved in Tumor Formation

Creighton¹,

Bromberg-White

Misek

et al. 2005

Molecular Cancer Research

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Tumor cell lines are relied on extensively for cancer investigations, yet cultured cells in an in vitro environment differ considerably in behavior compared with those of the same cancer cells that proliferate and form tumors in vivo. To uncover gene expression changes related to tumor formation, gene expression profiles of human lung adenocarcinoma (A549) cells grown as lung tumors in immune-compromised mice were compared with profiles of the same cells grown in vitro. Additionally, profiles of uninvolved adjacent mouse tissue were determined. A profound interplay between cancer cells and the host was shown that affected a complex protein interaction network involving processes of extracellular interaction, growth factor signaling, hemostasis, immune response, and transcriptional regulation. Growth in vivo of A549 cells, which carry an activating k-ras mutation, induced changes in gene expression that corresponded highly to a pattern characteristic of human lung tumors with k-ras mutation. Cytokines interleukin-4, interleukin-6, and IFN-; each induced distinct in vitro genomic responses in cancer cells that emulated many of the changes in gene expression observed in vivo. Genes that were both selectively induced in vivo and overexpressed in human lung adenocarcinoma tumors included CSPG2, which has not been associated previously with tumor formation. Knockdown in A549 of CSPG2 by RNA interference significantly inhibited tumor growth in vivo but not in vitro. Thus, analysis of tumor xenografts by gene expression profiling has the potential for identifying genes involved in tumor development that may not be expressed in cancer cells grown in vitro.

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Inhibition of anchorage-independent growth and lung metastasis of A549 lung carcinoma cells by IκBβ

Cited by 40 publications

References 29 publications

Identification of RASSF8 as a candidate lung tumor suppressor gene

Identification of RASSF8 as a candidate lung tumor suppressor gene

Nuclear factor-κB and inhibitor of κB kinase pathways in oncogenic initiation and progression

Analysis of Tumor-Host Interactions by Gene Expression Profiling of Lung Adenocarcinoma Xenografts Identifies Genes Involved in Tumor Formation

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