Inhibition of anaphylactic shock by gadolinium chloride-induced Kupffer cell blockade

Abstract

Data in the literature concerning the role of macrophages in anaphylaxis are contradictory. In the present study, the effect of macrophage blockade induced by gadolinium chloride (GdCl3) on anaphylactic shock is investigated. Our observations show that GdCl3 prevents lethal anaphylactic shock in mice sensitized to ovalbumin. Gadolinium chloride given i.v. in a dose of 1 mg/100 g body weight 24 or 48 h before the elicitation of anaphylactic shock resulted in 80% survival, compared with the 43% survival in the control group. The same dose of this rare-earth metal salt also greatly reduced the mortality in mice sensitized with ovalbumin containing Bordetella pertussis vaccine, and similarly abrogated the symptoms of anaphylaxis, including the accumulation of serotonin and histamine in the liver. The results suggest that macrophages play an important role in mouse anaphylaxis.

Pathways of immediate hypothermia and leukocyte infiltration in an adjuvant-free mouse model of anaphylaxis

“…Depending on the model used, MCs, basophils, monocytes/macrophages and/or neutrophils have been reported to contribute to ASA 11, 13, 14, 16–19, 21, 26, 35 . Several studies using passive or active models of anaphylaxis have reported an important role for monocytes/macrophages in the immediate hypothermia response following antigen challenge 5, 14, 17, 21, 26, 39–41 . We confirmed these findings in an ASA model using the adjuvants alum and Bordetella pertussis toxin for the sensitization (see Fig E4 in the Online Repository).…”

Kupffer cell phagocytosis blockade decreases morbidity in endotoxemic rats with obstructive jaundice

“…The Kupffer cell blockade induced by GdCl 3 is a generally accepted method for investigation of the physiological and pathophysiological roles of Kupffer cells. Potential beneficial effects of macrophage blockade have previously been demonstrated, such as the inhibition of anaphylaxis [39], and the development of lethal septic [15] and ETX shock [36,40]. GdCl 3 also influences the hypotension induced by immunoglobulin aggregates [41], and prolongs the survival of a human insulinoma cell xenograft in the liver [42].…”

A Kupffer-sejt gátlásának hatása az endotoxin indukálta gyulladásos válaszreakciókra és a máj mikrokeringési változásaira kísérletes obstructiós icterusbanr

“…Annak ellenére, hogy a KS-ek a reticulo-endothelialis rendszer fontos effektorsejtjei, a KS-gátlásnak számos kedvező hatása ismert. Így a KS-gátlás többek között gátolhatja az anafilaxiás shock kialakulását, csökkenti annak következményeit 15 , mérsékelheti a septicus 10,16 és/ vagy ETX okozta shock kialakulásának kockázatát és szövődményeit. Kísérleteinkben szintén a KS-gátlás számos kedvező hatását találtuk.…”