Inhibition of Anaphylactic Histamine Release and Pulmonary Distress in Rats by Nonspecific IgE

Butchko, G M; Aspinall, Richard L.; Smith, William G.

doi:10.1159/000233585

Cited by 7 publications

(12 citation statements)

References 11 publications

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“…Thus, whilst in the M-HSEL group a good agreement was found between skin test positivity and specific IgE antibody levels, in the low socioeconomic levels considerably higher proportions of the subjects had significant levels of IgE antibody but were not skin test positive. This suggests the existence of the mast cell block ade that has been shown to occur in situations of highly elevated polyclonal IgE [9,19,[20][21][22][23]. Confirmation of this possibility was obtained by P-K testing, as virtually all of the M-HSEL subjects tested were P-K positive,whereas al most half the persons of low socioeconomic level tested were negative.…”

Section: Discussionmentioning

confidence: 95%

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Allergic Reactivity of Children of Different Socioeconomic Levels in Tropical Populations

Hagel

Lynch

DiPrisco

et al. 1993

Int Arch Allergy Immunol

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Widely variable prevalences of allergic diseases have been reported in tropical populations, and this has been suggested to be due to effects of the nonspecific polyclonal stimulation of IgE synthesis caused by the helminthic infections that are endemic in these areas. Since 1980, we have been evaluating the allergic reactivity of different socioeconomic sectors of the population of tropical Venezuela (lat. 2–12°N), and in the present study analyze the overall results obtained in the laboratory evaluation of children (5–15 years of age) belonging to these groups. Children of medium-high socioeconomic level (M-HSEL), who experience occasional helminthic infections, have moderately high total serum IgE levels, and have elevated skin test positivities and specific IgE levels against environmental allergens. Persons of low socioeconomic level, in the urban, and particularly rural situation experience frequent helminthic infection, and have highly elevated total serum IgE levels. In contrast to the M-HSEL, the majority of these children have detectable specific IgE antibody against a variety of inhalant allergens, but relatively few have high levels, and their skin test positivity is also low. In these frequently parasitized persons, evidence of saturation of mast cell Fcε receptors was found by tests of passive sensitization. We propose, therefore, that helminthic parasites have a biphasic effect on allergic reactivity; occasional infections are stimulatory, via their nonspecific potentiation of IgE synthesis against environmental allergens, and frequent infections are suppressive due to the widely polyclonal stimulation that they cause, resulting in both diminished specific antibody production against any given allergen and mast cell Fcε receptor saturation.

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Section: Discussionmentioning

confidence: 95%

“…However, considerable evidence has been presented to suggest that highly elevated levels of polyclonal IgE can actually be suppressive, via a saturation of the mast cell Fct: receptors [9,[19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Allergic Reactivity of Children of Different Socioeconomic Levels in Tropical Populations

Hagel

Lynch

DiPrisco

et al. 1993

Int Arch Allergy Immunol

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“…In addi tion, pre-armed macrophages failed to transfer OVAsensitivity to IgE-myeloma-bearing rats. Saturation of receptors in these latter animals by high levels of endogenous IgE is known to inhibit skin sensitisation by exogenous reagins [25][26][27]. These observations argue against an active role for receptor-bound IgE on the macrophage surface in the expression of OVAinduced PCA reactivity at the injection site, as the functional capacity of such pre-armed receptors would not be influenced by extracellular IgE.…”

Section: Discussionmentioning

confidence: 99%

In vivo Arming of Cutaneous Mast Cell Receptors by IgE Released from Macrophages

Holt¹,

Bilyk²,

Vines³

et al. 1989

Int Arch Allergy Immunol

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Intracutaneous injection of purified peritoneal macrophages harvested from ovalbumin (OVA)-hypersensitive high-IgE-responder BN rats into naive animals sensitised the injection sites for subsequent OVA-specific passive cutaneous anaphylaxis (PCA) reactions. The underlying mechanism(s) were investigated using a macrophage cell line (WEHI 265.1), which exhibited comparable sensitising activity in rat or mouse skin, after initial pulsing in vitro with antiserum rich in OVA-specific IgE. Transfer of OVA-hypersensitivity by the cell line (1) was IgE-dependent and did not occur when the cells were pre-exposed to antiserum containing OVA-specific IgG alone, (2) was blockable by saturation of cell surface receptors in the recipient with myeloma IgE (but not myeloma IgG), and (3) did not occur in mast cell-deficient mice carrying the W/W^v mutation, in contrast to their normal heterozygous littermates which developed marked OVA-hypersensitivity at the injection site. These results are consistent with arming of IgE-receptors on cutaneous mast cells by IgE antibody released from macrophages, and hint at a possible role for phagocytes in amplifying IgE-mediated reactions in tissues.

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“…The present study examined the effect of three clinically useful calcium antagonists in a rat model of allergic pulmonary distress (APD). The model has been shown to be mediated by IgE, and thus appears to be a reliable indicator of clinical utility (1,3). Each calcium antagonist used in the present study significantly lessened the severity of the resultant allergic response.…”

mentioning

confidence: 67%

“…Animals were fasted overnight and restrained within a pneumotachograph-vented body plethysmograph incorporating a separate head chamber (1,3). The airflow signal from the pneumotachograph pressure transducer was integrated by a pulmonary-mechanics computer and digitized by a microprocessor (Buxco) to yield interval averages of minute volume (Vm).…”

Section: Measurement Of Anaphylactic Pulmonary Distressmentioning

confidence: 99%

The effect of calcium antagonists on allergic pulmonary distress in actively sensitized rats

Korach¹,

Carnathan²,

Aspinall³

1987

Allergy

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The induction of allergic pulmonary distress (APD) in ovalbumin sensitive rats can be used as a model of human allergic asthma. In this model, control animals exhibit a rapid decrease in minute volume (Vm) when challenged with ovalbumin (OA) by aerosol (3% solution). The present study compared the effects of pretreatment with calcium antagonists on the induction of APD. By the aerosol route of administration, 5 min before OA, verapamil HCl (6% solution) significantly (P less than 0.05) dampened the allergic response during all 12 min monitored. At an equivalent concentration, diltiazem HCl significantly (P less than 0.05) inhibited the response during 6 of 12 min, whereas nifedipine failed to significantly (P greater than 0.05) alter the response to OA. Verapamil and nifedipine proved to be equally effective in a dose-dependent manner against OA-induced APD, however, when administered orally (-60 min, 5, 10 or 20 mg/kg). At doses of 10 mg/kg and higher, both calcium antagonists consistently inhibited (P less than 0.05) the response. Diltiazem was inactive when administered orally at a dose as high as 20 mg/kg. The present data suggest that the calcium antagonists verapamil, nifedipine and diltiazem, can attenuate APD and therefore might be clinically active agents in the treatment of allergic asthma.

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Inhibition of Anaphylactic Histamine Release and Pulmonary Distress in Rats by Nonspecific IgE

Cited by 7 publications

References 11 publications

Allergic Reactivity of Children of Different Socioeconomic Levels in Tropical Populations

Allergic Reactivity of Children of Different Socioeconomic Levels in Tropical Populations

In vivo Arming of Cutaneous Mast Cell Receptors by IgE Released from Macrophages

The effect of calcium antagonists on allergic pulmonary distress in actively sensitized rats

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