Inhibition of African swine fever virus infection by genkwanin

Հակոբյան, Աննա; Arabyan, Erik; Kotsinyan, Armen; Karalyan, Zaven; Sahakyan, Harutyun; Arakelov, Vahram; Nazaryan, Karen; Ferreira, Fernando; Zakaryan, Hovakim

doi:10.1016/j.antiviral.2019.04.008

Cited by 47 publications

(28 citation statements)

References 23 publications

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“…Recent research suggested that the susceptibility to chemicals/disinfectants depends on viral characteristics in which non-enveloped viruses are more resistant than enveloped viruses [31]. Moreover, some chemical synthesis or extract from a plant such as an acacetin, apigenin, genkwanin, rhoifolin, vitexin, and vitexin 2-O-rhamnoside are able to inhibit or reduced ASF virus-speci c protein synthesis and viral factory formation in Vero cell line system, in which apigenin showed potent inhibition of ASF virusinfected Vero cells with not display a cytopathic effect [17,18,32]. However, there is no evidence of the survivability of ASFV in feed and water after pre-treatment with MCFAs.…”

Section: Resultsmentioning

confidence: 99%

“…The incorrect de nition of activity parameters such as concentration, contact time, and range may lead to the improper use of disinfectant products, whereby no effectiveness is achieved [11][12][13][14]. There are several substances or chemical compounds generally accepted as inactivating enveloped viruses, including the ASFV and the only studies on the use of disinfectants against the ASFV concerning tests on various surfaces, as revealed [15][16][17][18]. To the best of our knowledge, this is the rst in vitro testing of medium-chain fatty acids (MCFAs) as chemicals/disinfectants against the ASFV in swine feed and water models.…”

Section: Introductionmentioning

confidence: 99%

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The potential anti- African Swine Fever Virus effects of medium chain fatty acids on in vitro pig feed and water models

Tran¹,

Truong²,

Ly³

et al. 2020

Preprint

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Background African swine fever (ASF) is an important disease affecting swine and has a significant economic loss on both the developed and developing world but due to the lack of vaccines, drug, and effective control measures, ASF virus remains a serious threat to global pork production. The activities of medium-chain fatty acids (MCFAs) against viral pathogens have been reported previously. However, the effects of this family on ASFV have been not yet investigated. In this study, we evaluated the potential effects of MCFAs in individual and synergistic forms, to prevent and/or reduce ASFV infection using in vitro feed and water models. Results The potential effects of MCFAs, including C8, C6-C8-C10 (1:1:1 ratio) and C8-C10-C12 (1:1:1 ratio) against a field ASFV strain isolated in Red River Delta region of Vietnam were further examined by real-time PCR in in vitro feed and water models. All tested products have shown a strong antiviral effect against ASFV infectivity at doses of 0.375% and 0.5% in both feed and water assays. Interestingly, the synergistic MCFAs have shown clearly their potential activities against ASFV in which at lower dose of 0.25%, pre-treatment with product 2 and 3 induced significant increases at the level of Cq value when compared to positive control and/or product 1 (P < 0.05). Conclusions To our knowledge, it is the first report on in vitro examination of the anti-ASFV activities of the MCFAs. Our findings suggested that all tested products, both individual and synergistic forms of MCFAs, have possessed a strong anti-ASFV effect and this effect is dose- dependence in in vitro feed and water models. Additionally, synergistic effects of MCFAs are more effective against ASFV when compared to individual form. The further studies focusing on in vivo anti-ASFV effects of MCFAs are very important to bring new insight into the mode of ASFV-reduced action by these compounds in swine feed and water consumption.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The potential anti- African Swine Fever Virus effects of medium chain fatty acids on in vitro pig feed and water models

Tran¹,

Truong²,

Ly³

et al. 2020

Preprint

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show abstract

“…Therefore, it is necessary to develop antiviral drugs against the virus. Previous studies have found several antiviral drugs which could possibly inhibit ASFV infection in vitro (Arabyan et al 2019), including nucleoside analogues (Berry & Kinsella 2001), genistein (Arabyan et al 2018), genkwanin (Hakobyan et al 2019), Rifampicin (Dardiri et al 1971), fluoroquinolone (Mottola et al 2013), sulfated polysaccharides (García-Villalón & Gil-Fernández 1991), lauryl gallate (Hurtado et al 2008), stilbenes resveratrol (Galindo et al 2011), oxyresveratrol (Galindo et al 2011), histone deacetylases enzymes (HDACs) inhibitor (Frouco et al 2017), small peptide inhibitors (Hernáez et al 2010), and so on.…”

Section: Discussionmentioning

confidence: 99%

“…Manuscript to be reviewed antiviral drugs has identified targets and known mechanisms, and includes five kinds of drugs: i) nucleoside analogues, such as iododeoxyuridine (Gil-Fernández et al 1979), (S)-HPMPA (Gil-Fernández et al 1987), and Rigid amphipathic fusion inhibitors (Hakobyan et al 2018);ii) interferons (IFNs) and small peptides, such as IFN-alpha (Paez et al 1990), IFN-gamma (Esparza et al 1988) and small peptide inhibitors which could disrupt the interaction between cytoplasmic dynein and viral p54 protein (Hernáez et al 2010); iii) plant-derived compounds, such as genistein (Arabyan et al 2018) and genkwanin (Hakobyan et al 2019). The former interferes with viral type II topoisomerase and the latter disrupts the virus movement along microtubules.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Prediction of antiviral drugs against African swine fever viruses based on protein–protein interaction analysis (v0.1)"

2020

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The African swine fever virus (ASFV) has severely influenced the swine industry of the world. Unfortunately, there is currently no effective antiviral drug or vaccine against the virus. Identification of new anti-ASFV drugs is urgently needed. Here, an up-to-date set of protein-protein interactions between ASFV and swine were curated by integration of protein-protein interactions from multiple sources. Thirty-eight swine proteins were observed to interact with ASFVs and were defined as ASFV-interacting swine proteins. The ASFV-interacting swine proteins were found to play a central role in the swine proteinprotein interaction network, with significant larger degree, betweenness and smaller shortest path length than other swine proteins. Some of ASFV-interacting swine proteins also interacted with several other viruses and could be taken as potential targets of drugs for broad-spectrum effect, such as HSP90AB1. Finally, the antiviral drugs which targeted ASFV-interacting swine proteins and ASFV proteins were predicted. Several drugs with either broad-spectrum effect or high specificity on ASFV-interacting swine proteins were identified, such as Polaprezinc and Geldanamycin. Structural modeling and molecular dynamics simulation showed that Geldanamycin could bind with swine HSP90AB1 stably. This work could not only deepen our understanding towards the ASFV-swine interactions, but also help for the development of effective antiviral drugs against the ASFVs.

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“…With the FRET pair, a flavonoid library was screened to search ASFV protease inhibitory compounds. Recent studies showed that flavonoids have antiviral activity in some viruses including ASFV [12][13][14] . However, none of the antiviral studies with specific target protease at the molecular level has been reported.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of African swine fever virus protease by myricetin and myricitrin

Kim

Shin

et al. 2020

Journal of Enzyme Inhibition and Medicinal Chemistry

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African swine fever (ASF) caused by the ASF virus (ASFV) is the most hazardous swine disease. Since a huge number of pigs have been slaughtered to avoid a pandemic spread, intense studies on the disease should be followed quickly. Recent studies reported that flavonoids have various antiviral activity including ASFV. In this report, ASFV protease was selected as an antiviral target protein to cope with ASF. With a FRET (Fluorescence resonance energy transfer) method, ASFV protease was assayed with a flavonoid library which was composed of sixty-five derivatives classified based on ten different scaffolds. Of these, the flavonols scaffold contains a potential anti-ASFV protease activity. The most prominent flavonol was myricetin with IC 50 of 8.4 lM. Its derivative, myricitrin, with the rhamnoside moiety was also showed the profound inhibitory effect on ASFV protease. These two flavonols apparently provide a way to develop anti-ASFV agents based on their scaffold.

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Inhibition of African swine fever virus infection by genkwanin

Cited by 47 publications

References 23 publications

The potential anti- African Swine Fever Virus effects of medium chain fatty acids on in vitro pig feed and water models

The potential anti- African Swine Fever Virus effects of medium chain fatty acids on in vitro pig feed and water models

Peer Review #2 of "Prediction of antiviral drugs against African swine fever viruses based on protein–protein interaction analysis (v0.1)"

Inhibition of African swine fever virus protease by myricetin and myricitrin

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